How to observe blood cells under the microscope?

The crack is because you didn't spread the blood, a large blood clot was on the slide, and then it dried up and shrank and cracked.

translucent discs", which are red blood cells.

White blood cells, very few, just like your poor film can not be seen, smear staining is good.

Platelets, very small. When the film is made, it is difficult to recognize when it is dyed and colored, and it looks like a fragment.

I'll take a look at the methods of smear staining, it's all in the book.

1. Take a drop of blood and place it at one end of the slide, hold the slide in your left hand, and move the end of the slide with a smooth edge in your right hand to touch the blood droplet, and the blood droplet will spread along the push piece. Then the angle between the slide and the slide is kept at 30-45 degrees to move forward smoothly, and a thin layer of blood film is retained on the slide.

2. After the blood smear is made, you can hold the slide and wave it in the air to make the blood film dry quickly, so as to avoid the shrinkage of blood cells.

3 Use crayons to draw lines on both sides of the blood film to prevent the dye from spilling, and then lay the blood film flat on the staining rack. Garry's stain solution 2-3 drops to cover the entire blood film for a fixed min. Add an equal or slightly more amount of fresh distilled water dropwise and mix with the dye for 5-10 min.

4. Rinse off the stain with water, dry it naturally or blot it with absorbent paper, and then place the blood smear under the microscope for microscopic examination.

Neutrophils are shown in the picture. There are many kinds of granulocytes that are less than half of them.

Common neutrophils, lymphocytes, eosinophils, monocytes and basophils (in descending order of normality), you can only upload one picture here, and you can take a look at the rest.

To see blood cells, it is generally observed with a thousand-fold oil mirror...

Yes, it can be easily observed after staining, and can be observed using iodine solution staining, methyl blue or Gram staining. The light microscope can only see the cell structure, what organelles and the like, and what it looks like clearly, but the electron microscope can see the submicroscopic structure. If the blood droplet is very thick, it will affect the transmittance of the sample during observation, and the blood smear is made to ensure the stability of the light source of the sample and the clearer observation.

The blood smear is easy to do, pay attention to the size of the blood droplet, the angle of inclination of the coverslip, and the speed at which the piece is pulled.

Under the light microscope, the organelles that can be directly observed are the nucleus, chloroplast, vacuole, centrosome; The organelles that need to be stained under light microscopy to be observed include mitochondria, Golgi apparatus, endoplasmic reticulum and other larger organelles. Other very small organelles, such as ribosomes, can only be observed under an electron microscope. Red blood cells and bacteria can be observed with a normal microscope. The low magnification lens 100 can see red blood cells, and 400 times can see more clearly.

The field of view is very small, and it needs to be enlarged by chemicals, and it needs to be stained, otherwise the bacteria cannot be seen.

The magnification of a normal microscope is the magnification of the eyepiece (the lens through which the eye observes) x the magnification of the objective lens (the lens that is close to what is being observed). For example, the eyepiece magnification is 10 times, and the objective lens is 40 times magnification, which is 400 times magnification, which is already a high magnification. A microscope can be combined with lenses to form different magnifications.

Magnification to 1000x is almost the limit of magnification for optical microscopes.

Because the resolution of light microscopy is still too low compared to electron microscopy, it can observe the cell membrane, cytoplasm, and the organelles that can be seen are mitochondria, chloroplasts, vacuoles, and the complete structure of the nucleus. As for why these organelles can be seen, because chloroplasts are green and they are more widely distributed, they may be present in mitochondria, vacuoles, so mitochondria as well as vacuoles can be seen. As for the nucleus, only the general structure can be seen, and the nuclear membrane and nuclear pores cannot be observed.

Yes, you can use an ordinary light microscope to see some more conventional cells, which are very clear and have different colors.

Cells can be seen, and science and technology are becoming more and more advanced, so very small cells can be seen through such a microscope.

Yes, but the part you can see is very limited, you can't see the very detailed part, and if you use an optical microscope, you can see more detailed places.

Confocal microscopy can be selected for live-cell imaging, and the principle difference between confocal and traditional microscopes lies in the different illumination methods: traditional microscopes illuminate the entire field of view of the sample at one time, so that images can be directly observed by eye or acquired with CCD without time delay; On the other hand, confocal microscopy is point-by-point imaging, and the image cannot be obtained with CCD, so the signal of each pixel point can only be collected with a detector, and then the image is reconstructed by software, which has a certain time delay.

Confocal microscopy is used for cell morphological analysis (observation of the morphological characteristics of subcellular structures such as intracellular mitochondria, endoplasmic reticulum, Golgi apparatus, microtubules, microfilaments, cell bridges, chromosomes, etc.); semi-quantitative immunofluorescence analysis); fluorescence in situ hybridization studies; Gene mapping studies and 3D reconstruction analysis.

Confocal microscopes from Leica Microsystems are widely used for surface analysis in biomedical research and materials science applications, providing researchers with high-precision 3D imaging data as well as accurate imaging of subcellular structures and dynamics. Leica laser confocal microscopes are designed based on a modular concept and offer flexible upgrades that integrate innovative features including STED super-resolution imaging, DIVE tunable spectral deep tissue imaging, and more.

Operation: First, to observe the cells, we first need to make a mounting.

Temporary film production.

1. Drop a drop of water on the ** of the glass slide, put the material into it with tweezers, cover the coverslip and use absorbent paper to absorb the surrounding water and wipe it clean before putting it on the stage for observation.

2 If there are air bubbles, you can use a dropper to add water on one side of the coverslip and use absorbent paper to suction the air bubbles on the other side.

3 The stage must be kept level to avoid clean water flowing out of the stage and polluting the stage.

2. Observe the mounting.

How it works: 1. Adjust the brightness: from dark to bright, you can use a large aperture, a concave mirror, and adjust the angle of the reflector.

2 Fix the temporary loading in the appropriate position on the stage.

3 The low-magnification objective lens is aligned with the clear hole, and the lens barrel is adjusted from top to bottom using a coarse collimation spiral, so that the eyes are on the side to avoid the objective lens from touching the slide and damaging the lens and crushing the slide.

4 The left eye observes the changes in the visual field through the eyepiece, and at the same time adjusts the coarse collimation spiral so that the lens barrel slowly moves up until the field of vision is clear.

5 If there is no object to be observed in the field of view, you can move the film, the principle is to go up and down, and to go left and right.

6 If it is not clear enough, it can be further adjusted with a fine collimation spiral.

7 If you need to observe under a high-magnification objective, you can turn the converter to change the objective. If the visual field is dark, it can be adjusted by method 1; If it is not clear enough, it can be adjusted by 6 method, but not by 4 method.

8 After use, adjust the converter so that the empty lens hole is facing the clear hole, make the reflector stand upright, adjust the lens barrel to the lowest level, and put it into the lens case.

Question: Red blood cells under the microscope? The question you mentioned.

It is supposed to be a human red blood cell as seen in an ordinary light microscope: red blood cells are disc-shaped with depression on both sides.

Non-nucleated cells.

The side view is dumbbell-shaped.

Commonly known as salty pancakes).

Its diameter is about microns.

The thickness of the perimeter is about microns.

The thickness of the center is about microns.

The reason why it is "red" as seen by ordinary light microscopes is because of the color of hemoglobin.

The middle part is brighter or whiter.

is that its center thickness is only about microns.

Contains less hemoglobin. Red blood cells this form.

It has its physiological significance.

This is because this form (a double-sided concave disc) has a larger surface area than a sphere of the same volume.

It is conducive to the oxygen-carrying function of red blood cells.

Reference: Physiology textbook (for medical colleges).

Red blood cells can be seen with an ordinary light microscope, and the reason why they are lighter is because the red blood cells do not have a nucleus, which makes them become a left biconcave round pie shape, and according to the shape, the middle of the red blood cells is thinner, and it is naturally more transparent, so there is more light under the reflected light of the light microscope, and it becomes lighter.

Reference: Microscope experience.

Most of the microscopes we look at now are seen by electron microscopes. However, there are no colors in the electron microscope, and these colors are meant to make people see more clearly. And that explains why the middle part of the red blood cell under the microscope is brighter, just because of the coloring.

It is also possible that the red blood cells are recessed in the middle < - for reference).

Reference: Probably so.

Question 1: What microscope is better for general cell culture observation in universities An inverted microscope is good for ordinary cell culture observation in universities.

Inverted microscopes and magnifying glasses serve the same purpose, by turning a small object in the near distance into a magnified image for the human eye to observe. It's just that a microscope can have a higher magnification than a magnifying glass.

Object ** in front of the objective, the distance from the objective lens is greater than the focal length of the objective, but less than twice the focal length of the objective. Therefore, after it passes through the objective lens, it will inevitably form an inverted magnified real image A'b'。a'b'Near F2.

It is then magnified by the eyepiece to a virtual image A''b''After eye observation. The eyepiece serves the same purpose as a magnifying glass. The only difference is that what the eye sees through the eyepiece is not the object itself, but the image of the object that has been magnified once by the objective.

Question 2: What is the name of the scientist who first used a microscope to observe cells The first person to discover a cell with the help of a homemade microscope and named it was the British scientist Hooke, who actually observed that the "chambers" called cells were dead cells with only the cell wall remaining.

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When looking at a blood smear under a microscope, the largest number of red blood cells <>< seen

The number of red blood cells per cubic millimeter of blood in adults is about 5 million for men and about 4.2 million for women. There are many kinds of white blood cells, with a nucleus, which are larger than red blood cells and are less numerous, with 5,000-10,000 per cubic millimeter of blood.

1.After installing the slide, plug in the power of the microscope, press the light source switch, adjust the light source to the maximum brightness (here is a small trick to help you roughly determine the position of the image) so that the light shines through the light aperture to the slide, and observe from the side whether the position of the light projection is aligned with the position of the sample. If an unstained specimen is being observed,..

2.Next, find the image at low magnification and move the position of the sample slightly, at which point the brightness of the light source should be dim.

3.Finally, use a high magnification lens, at this time the brightness of the light source should be adjusted to the maximum, because the number of cells is small, the brightness of the field of view is low, and the brightness of the field of view should be increased.