Monoclonal antibodies are prepared from cells

In practice, all B cells were extracted from the spleens of immunized mice, including B cell nuclear effector B cells (a variety of types) mentioned in Compulsory 3. The reason is the presence of multiple epitopes on the surface of xenoantigens), which then induce these cells to fuse with mouse myeloma cells.

After fusion, three major types of cells can be obtained: tumor-tumor fusion cells, B-B fusion cells, and hybridoma cells (tumor-B fusion cells).

Then there are two screenings:

First screening: hybridoma cells were obtained using selective medium that could only be hybridoma cell survival;

The second screening: the specific antibody test is positive (Li Suyang antigen-antibody hybridization principle) to obtain hybridoma cells that can produce specific antibodies.

Effector B Cells Effector B cells are a type of lymphocyte that secretes specific antibodies, and the purpose of making monoclonal antibodies is to obtain specific antibodies in large quantities.

Monoclonal antibodies are produced after the fusion of effector B cells and myeloma cells.

Effector cells are responsible for the production of monoclonal antibodies, and myeloma cells are responsible for cell immortality.

In addition, effector B cells are also B cells, and the data is correct.

Answer: Hybridoma cells that have been screened and cloned can only synthesize and secrete specific antibodies to a single epitope, which is a monoclonal antibody. The production process of monoclonal antibody is roughly divided into antigen preparation, animal immunity, fusion of B cells and myeloma cells to form hybridoma cells, screening hybridoma cells, screening hybridoma cells that can produce a specific monoclonal antibody, cloning of hybridoma cells, large-scale culture in vitro or animal intraperitoneal culture of specific hybridoma cell cloning, purification and identification of monoclonal antibodies.

(1) Clonal selection theory of lymphocytes producing antibodies, that is, one clone produces one antibody.

2) Hybridoma cells are hybridized with myeloma cells to produce hybridoma cells, maintaining the characteristics of antibody production and indefinite reproduction by the parental cells of both sides.

3) Hybridoma cells were screened out by the metabolic defect rescue mechanism, and cloned by limiting dilution, and then cultured and proliferated in large quantities to prepare various monoclonal antibodies required.

Answer]: The most commonly used melting aid in the preparation of monoclonal antibodies is PEG, and the concentration is 30 50.

1.Mice are injected with specific antigenic proteins to produce an immune response;

2.Obtain the corresponding B lymphocytes;

3.Mouse myeloma cells were fused with B lymphocytes and then screened with a specific selection medium.

4.On this medium, both unfused parental cells and fused cells with an allonuclear die, and only fused hybrids can grow (which are characterized by both rapid proliferation and specific antibodies);

5.The above-mentioned hybridoma cells also need to be clonalized and cultured and antibody tested, and after multiple screenings, a sufficient number of cells that can secrete the required antibodies can be obtained;

6.Finally, hybridoma cells are cultured on a large scale under in vitro conditions or injected into the mouse intraperitoneal cavity for proliferation, so that a large amount of monoclonal antibodies can be extracted from the cell culture medium or mouse ascites fluid!

(1) Animal cell culture (microbial culture).

2) Select. 3) Strong specificity and high sensitivity.

4) Inactivated virus.

5) proliferate in large quantities in vitro secretion of specific antibodies (6) bone marrow hematopoietic stem.

7) Active transport (active absorption).

When a B lymphocyte is fused with myeloma cells that can proliferate in vitro, the resulting fusion cells can proliferate in large quantities to produce a sufficient number of specific antibodies.