What is the difference between a WAINCOM sequencer and a SOLID sequencer?

Solid after a round of connection, cut off a few bases, but will also keep a few bases, there will be an "extension", this online information is a lot, just look for it.

Looking at the relevant patents of Huainkang, it is approximately: the first round of connecting a 10mer (for example, ngcatgcata) probe, the next round of ligation reaction will cut off all the 10 bases, and then replace the new probe (ancatgcata), and so on, how long the probe is, it can only measure how long, as for the accuracy, you can imagine.

The BGISEQ-500 (codenamed Zebra in internal development) is subtle from the naming, and it looks like the direct competitor is the nextseq 500 from Illumina. There were also two domestic sequencers before, Zhongke Zixin BIGIS-i and Huayinkang PSTAR-II, in this post, I don't think it's better to take it out to be ridiculed and disgusted by the old club. Main parameters:

Throughput: BGISEQ-500: 8-200g, NextSeq 500:

25g-120g Read: BGISEQ-500: 50SE 50PE 100SE 100PE, NextSEQ 500:

75SE 75PE 150PE Quality: BGISEQ-500: Q30 bases > 85%, NextSeq 500:

12-30h can be said to be, two 500 all-round frontal rigid! The nextseq 500 is primarily targeted at small- and medium-scale applications at the laboratory level, unlike HISEQ, which is positioned in sequencing plants, and MISEQ is positioned for high-precision and rapid scientific research in the laboratory. The throughput can be up or down, and it can do human whole genome sequencing, and it can also do nifty.

Judging from the official data, the two 500 have their own collars, each with its own merits.

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From a safety point of view, no other substances can be added to the mouth of the condenser tube. Otherwise, it is a violation.

1. If the reaction is smooth, it may cause the blockage of the condenser tube in this section. Affects the reflux of condensate substances.

2. If the reaction is violent, it may cause the condenser tube to be violently heated, colded, and impacted in this section, resulting in safety accidents.

Moreover, if a substance is added to the condensation port, even if there is no safety hazard, the reaction speed of the two substances must be problematic, and the result is that the reaction is not sufficient, and there may be a variety of ingredients in the refluxed substance, which has an impact on the purpose of the chemical reaction you want to achieve.

Since it is called the condensation port, it is absolutely not possible to add reactants, if the pressure of the condensation port is blocked, there are many dangers, small damage to the equipment, if the large is a pressure vessel, it may cause **, add reactants from the addition port, in strict accordance with the operating procedures to avoid unnecessary accidents, each equipment has its own requirements and operating specifications, every accident is a lesson of blood, each operating procedure is a summary of blood!

This should be viewed from two aspects, of course, it is unreasonable from the standard operation in the textbook, but in fact, in the actual operation of simple experiments, the condenser tube feeding is very common, but it is not safe, and the reactants are not safe to be partially bonded to the condenser tube, or it is not easy to clean.

It depends on the specific situation and cannot be generalized. If you don't know much about the reaction, it's best not to do it. There are a lot of rules for people who don't understand or can't control it, if you think you know the whole reaction well, and there will be no consequences such as coagulation, adhesion, explosion and dissolution, etc., and there is no impact on the human environment and equipment, then you can operate.

Normally, from the standard operation in the textbook, it is definitely not, and it is easy to solidify and block the condenser tube; Violent reactions can be dangerous because the speed and flow are uncontrollable. But in fact, in the actual operation of simple experiments, condenser tube feeding is very common, and some operations are reasonable but not standardized, but convenient, but safety first.

I don't think there's anything you can't do, as long as it's not easy to solidify.

Otherwise, it will be difficult to wash the solidification and hardening on the condenser tube.

In addition, the reaction should not be too violent, because the speed and flow rate of the reactants added from the condenser tube are uncontrollable, and if the reaction is too violent, it will come out.

Others should not matter, some operations are reasonable but not legal.

Science and engineering, intelligent instruments, principles of chemistry.

This should be viewed from two aspects, the reactants are partially bonded to the condenser tube and are not safe, so it is not recommended.

Probably not, because the condenser tube is used for the reflux of the liquid in the reaction device, so that the reaction is more thorough.

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The theme of the work is to talk about the strange **Illustration.

Measurement and control itself is a major, there are many things to learn, opto-mechanical and electrical computing, there is a lot of pressure, a lot of class hours, the first and second year public basic courses, the third and fourth year professional courses and professional elective courses; Basically all physics, mechanics is divided into: material mechanics, theoretical mechanics; Optics: Applied Optics, Physical Optics, Optical CAD, etc.; Electricity:

analog circuits, digital circuits; Mechanical: engineering drawing, mechanical principles, etc.; Computer: C language, single-chip microcomputer, microcomputer principle; There are also professional electives in various directions after the fourth year, there are too many to list one by one, such as optical technology and testing technology.

All of the above subjects will be studied, scattered in the freshman to senior year, we were sophomores and juniors, basically every day from morning to evening classes, from 8 o'clock in the morning to 6 o'clock in the evening, the pressure of the exam is also greater than that of other colleges, once four days in a row 7 subjects, basically means, every day to stay up late to read.

wish you luck

Changchun University of Science and Technology is a general school, but the major of measurement and control technology and instrumentation is very good! The focus is on computer technology, measurement and control technology as the main line, the electronic information technology and computer technology, measurement and control technology and other organic combination, the design of the measurement and control system and the modern measurement and control system for microcomputer dynamic data acquisition and signal analysis and processing, and the test and control system applied to the intelligent instruments design.

Measurement and control belongs to a new type of major, in the past, only graduate students had this discipline, and now the undergraduate has also opened this discipline, and it is on the rise This is a wide-caliber major, and the future development direction is control theory and control engineering, instrument science and technology. In the future, we will focus on the development of intelligent instrumentation, and the future is very good. Business Training Objectives:

This major cultivates senior engineering and technical talents who have the basic knowledge and application ability of precision instrument design and manufacturing, measurement and control, and can be engaged in the design and manufacture of relevant technologies, instruments and systems in the field of measurement and control, scientific and technological development, applied research, operation management and other aspects in various departments of the national economy. Business training requirements: Students in this major mainly learn the basic theory of optics, mechanics and electronics of precision instruments, measurement and control theory and design methods of related measurement and control instruments, and are trained in modern measurement and control technology and instrument application, and have the ability to apply and design and develop measurement and control technology and instrument systems in this specialty.

Graduates should acquire the following knowledge and abilities: 1. Have a solid foundation in natural sciences, humanities, arts and social sciences, and the ability to correctly use the Chinese language and writing; 2. Systematically master the basic knowledge of a wide range of technical theories in the professional field, mainly including basic knowledge of mechanics, electronics, optics, measurement and control, market economy and enterprise management; 3. Master the contemporary measurement and control technology and experimental research ability combining optical, mechanical, electrical and computer, and have the ability to design and develop the measurement and control technology, instruments and systems of the specialty; 4. Strong foreign language skills; 5. Have strong self-learning ability, innovation consciousness and high comprehensive quality. Main disciplines:

Optical Engineering, Instrument Science and Technology. Main courses: Precision Mechanics and Instrument Design, Precision Mechanical Manufacturing Engineering, Fundamentals of Analog Electronic Technology, Fundamentals of Digital Electronic Technology, Principles and Applications of Microcomputers, Fundamentals of Control Engineering, Signal Analysis and Processing, Precision Measurement and Control and Systems, Engineering Optics.