What is the difference between biuret and biuret reagents?

1. The role is different

Fillin's reagent is a newly formulated solution that reacts with an aldehyde group under heating conditions and is reduced to a brick-red precipitate that can be used to identify the presence of soluble reducing sugars. When the soluble reducing sugar is identified with Filin reagent, the color change process of the solution is: light blue, brown, brick red (precipitate).

When identifying whether a protein is present in a biological tissue, the biuret method is commonly used, using a biuret reagent, and a biuret reaction occurs. The diuret reaction is essentially a purple reaction with the biuret reagent in an alkaline environment. The protein molecule contains many peptide bonds that are similar to the structure of biuret, so the protein can have a color reaction with the biuret reagent.

2. The configuration method is different

Preparation method of Filin reagent: G mi NaOH (solution A) and G mi CuSO4 (solution B). The solution was prepared by dissolving 50 g of sodium hydroxide and 137 g of potassium sodium tartrate in 500 mi of distilled water (stored in a bottle with a rubber stopper).

The preparation method of B solution is to dissolve g crystalline copper sulfate in 500 ml of water and add MI sulfuric acid. Mix well.

Biuret reagent A is an aqueous solution of NaOH with a mass fraction of g ml; Biuret reagent B is a cuSO4 solution in water with a mass fraction of g ml. First, add 3ml of biuret reagent A to the solution to be tested with uniform shaking (to create an alkaline environment), and then add 1 2 drops of biuret reagent B with uniform shaking.

3. Different ways to use:

When using the Filin reagent, the solution is mixed with the solution (drops of the solution are dropped into the solution) and then used immediately.

When using the biuret reagent, first add the solution (2ml), shake and shake well to create an alkaline reaction environment, and then add 3 or 4 drops of the solution, shake and shake well to observe the phenomenon.

The biuret reagent is composed of two reagents: biuret A and biuret reagent B.

The composition of biuret reagent A is an aqueous solution with a mass fraction of sodium hydroxide in g ml;

The composition of biuret reagent B is an aqueous solution of copper sulfate with a mass fraction of g ml.

The biuret reagent can verify the presence of proteins.

Here's how:

First, add biuret reagent A to the tissue sample solution and shake evenly, and then add biuret reagent B and shake evenly. If the tissue contains protein, you will see the solution turn purple. Compounds with two or more peptide bonds can react purple with biuret reagents.

The peptide bond of the protein can be complexed with Cu2+ in alkaline solution to form purplish-red compounds. The shade of color is directly proportional to the protein concentration.

Agree with the second floor that biuret is the name of a chemical substance, while biuret.

A reagent is the name of a reagent.

The meaning is as follows:

The biuret method is an analytical method used to identify proteins. Shuangshan Punch Urea Reagent.

It is an alkaline copper-containing test solution that is blue in color and consists of 1% potassium hydroxide.

A few drops of 1% copper sulfate.

and potassium sodium tartrate.

When the substrate contains peptide bonds.

When (polypeptide), the copper in the test solution is coordinated with the polypeptide, and the complex.

Purple in color. Concentrations can be analyzed colorimetrically, with a wavelength of 540 nm in the UV-Vis spectrum. The sensitivity of the identification reaction is 5-160 mg ml. Identification of reaction protein units 1-10 mg.

Introduction: Funny Bi Jian

Protein is the material basis of life, an organic macromolecule, the basic organic matter that makes up cells, and the main bearer of life activities. There is no life without protein. Amino acid.

is the basic building block of proteins. It is a substance that is closely linked to life and to all forms of life activities.

Every cell and all important components in the body are involved in proteins. Protein accounts for 16% to 20% of the body's weight, which means that an adult weighing 60kg has about protein in his body. There are many types of proteins in the human body, with different properties and functions, but they are all composed of 20 kinds of amino acids (amino acids) combined in different proportions, and are constantly metabolized and renewed in the body.

1. The biuret reaction is unique to peptides and proteinsAmino acid. There is no one kind of color reaction. Generally, compounds containing two aminomethyl caseinyl groups in the molecule react with an alkaline solution to produce a purple or blue-purple colorComplexes。2. In the alkaline solution, the biuret reacts with copper ions to form a purple-red complex, which is the biuret reaction.

The biuret reaction is a color reaction that is unique to peptides and proteins, but not found in amino acids. Generally, the molecule contains two aminoformosyl groups (i.e., peptide bonds.

co-nh-) reacts with an alkaline solution to form a purple or blue-violet cracked complex.

In alkaline solution (NaOH), diurea biscination (H2NOC-NH-CONH2) reacts with copper ions (Cu2+) to form a purple-red complex (the molecular structure of the substance that is first disordered.

See Fig.), which is the biuret reaction.

When the copper solution reacts, a purple or blue-purple complex is formed.

Note: In addition to -co-nh-, amide (-conh2), methylene (-ch2-), imino (-nh-), cs-cs-nh2) and other groups also have this reaction.

Biuret reaction is a chemical reaction that is commonly used to detect proteins. It is one of the methods that uses biuret as a reagent to form a purple complex by combining the reagent with the peptide bond in the protein to form a purple complex, so as to quantitatively or semi-quantitatively detect the protein content.

In the diuret reaction, a purple complex is formed after the sample reacts with the diuret mixture, and the depth of the dull color of the complex is directly proportional to the protein content in the sample. Therefore, the amount of protein in the sample can be estimated based on the depth of the reaction solution. This method is a common, simple, rapid and accurate method for the determination of protein kullin, which is widely used in biochemistry, molecular biology, clinical chemistry and other fields.