Introduction to bovine serum albumin, what is bovine serum albumin used for?

Bovine serum albumin (BSA) is a globulin in bovine serum, also known as the fifth component (it should be noted that the fifth component is not a component of BSA, but Edwin J.).

Professor Cohn early obtained 5 components from blood by specific isolation techniques, the fifth of which is albumin), CAS number 9048-46-8, containing 583 amino acid residues, and molecular weight.

kda, the isoelectric point is. Bovine serum albumin has a wide range of applications in biochemical experiments, such as as as a blocking reagent in western blot.

Uses: 1. It helps the stability and activity of Taq enzyme in PCR system, and can improve the efficiency of PCR.

2. It is also often used in ELISA, such as blocking solution, sample diluent, and enzyme conjugate diluent can be used BSA.

3. Biochemical research, genetic engineering and pharmaceutical research.

4. Albumin in blood mainly plays the role of maintaining osmotic pressure, pH buffering, carrier and nutrition, and in the serum-free culture of animal cells, the addition of albumin can play a physiological and mechanical protective role and a carrier role.

Referenced from Absin

Serum protein (protein number 1E7I) is a carrier of fatty acids in the blood. Fatty acids are important for the body in two ways.

They are the building blocks of lipids, which in turn make up all the biological membranes around and within the cell; They are an inexhaustible source of energy. Our bodies have a storehouse of fatty acids – fat.

When the body needs energy or building materials, fat cells release fatty acids into the bloodstream.

Fatty acids are obtained by serum proteins and transported to the site where they are needed.

Serum protein is the most abundant protein in plasma. Each protein molecule can carry seven fatty acid molecules. These fatty acid molecules bind in the crevices of the proteins, and their carbon-rich tails are buried inside to safely avoid the surrounding water molecules.

Serum proteins are also capable of carrying many other water-insoluble molecules. Serum proteins, in particular, can carry many drug molecules, such as ibuprofen.

Because serum protein is so ubiquitous in the blood and so easily purified, it became one of the first proteins studied by scientists. Today, when a protein is needed, a similar protein in cattle is widely used in research, and this protein is called bovine serum protein or BSA. Many enzymes are unstable in dilute solutions, and the solution is to add some bovine serum proteins.

In the test, it makes the enzyme stable and relatively neutral, without affecting the properties of the enzyme.

Bovine serum albumin (BSA) is a globulin in bovine serum, containing 607 amino acid residues, molecular weight, and isoelectric point. Bovine serum albumin has a wide range of applications in biochemical experiments.

1. Used in biochemical research, genetic engineering and pharmaceutical research.

2. Used as medicine and health food and condiments.

3. Maintain osmotic pressure, pH buffer, and carrier function.

4. It helps the stability and activity of Taq enzyme in the PCR system, and can improve the efficiency of PCR Add 100 ml of water per 10 grams to dissolve, or dissolve with PBS, depending on the use. Then divide into small branches. If no preservatives are used, they can be stored in a thermostatic cabinet at minus 20 or 30 degrees Celsius, and if preservatives (such as sodium azide) are used, they can be stored at minus 4 degrees Celsius.

Generally, it can be stored for several months without deterioration. Preservatives may affect the effect of bovine serum albumin and should be used with caution.