Ion exchange chromatography, affinity chromatography, and high performance liquid chromatography whi

Answer]: Principle: Use the difference in the adsorption force of each mold member in the mixture in the two phases for separation.

Application: Silica gel separates various components. Alumina separates basic or neutral lipophilic components.

Activated carbon separates water-soluble components. Polyamide is an intermolecular hydrogen bond adsorption, which is suitable for separating flavonoids, quinones, and phenolic components. In the production of industrial closed punching industry, macroporous adsorption resin is commonly used for desalination, desugarization and protein treatment.

Summary. They are all the same in principle, but the difference is the force between the mobile phase and the chromatographic agent.

Gel filtration, also known as zeolite chromatography, uses microporous gels to separate components of different molecular weights.

Ion exchange is the use of the electrostatic interaction between the charge of the separated substance and the ionic groups on the chromatography agent.

Compare the differences between gel filtration chromatography and ion exchange chromatography.

In the original dermathetic, they are all the same, the difference is the difference in the force between the mobile phase and the chromatographic agent. Gel filtration, also known as zeolite chromatography, uses microporous gels to separate components of different molecular weights. Ion exchange is the use of the electrostatic interaction between the charge of the separated substance and the ionic groups on the chromatography sensitizer.

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Adsorption chromatography.

Adsorption chromatography uses the difference in the adsorption capacity of substances and molecules in stationary phase adsorption to achieve the separation of mixtures, and the chromatographic process of adsorption chromatography is the process in which mobile phase molecules compete with substance molecules for the adsorption center of the stationary phase.

The partition coefficient expression for adsorption chromatography is as follows:

where xa represents the molecular content of the component adsorbed to the active center of the stationary phase, and xm represents the molecular content of the component free in the mobile phase. The partition coefficient is important for calculating the retention time of the components of the substance to be separated.

Dispense chromatography. Partition chromatography takes advantage of the difference in solubility of the components to be separated between the stationary and mobile phases. The stationary phase of chromatography is generally a solvent in the liquid phase, which is distributed on the surface of the column or support body by means of cloth, bonding, adsorption, etc. The process of partition chromatography is essentially a process in which the molecules of the components are continuously dissolved in equilibrium between the stationary and mobile phases.

The expression of the narrow partition coefficient for the partition chromatography is as follows:

where CS represents the solubility of the component molecules in the stationary phase liquid, and CM represents the solubility of the component molecules in the mobile phase.

Ion-exchange chromatography.

Ion-exchange chromatography takes advantage of the difference in the ability of the separated components to be separated by the ability to exchange ions between the stationary phase. The stationary phase of ion exchange chromatography is generally an ion exchange resin, and there are many ionized active centers in the molecular structure of the resin, and the ions in the components to be separated will be ion-exchanged with these active centers to form an ion exchange equilibrium, thus forming a partition between the mobile phase and the stationary phase. The intrinsic ions of the stationary phase compete with the ions in the components to be separated for the ion exchange center in the stationary phase and move with the movement of the mobile phase to achieve the final separation.

The partition coefficient of ion-exchange chromatography is also called the selection coefficient, and its expression is:

Answer]: Test point: The principle of ion exchange chromatography. Ion exchange chromatography is an ion exchanger, which achieves the purpose of separation according to the different affinities of each component and ion exchanger.