Are there microorganisms in distilled water? Why?

Theoretically, there are no microorganisms in distilled water, because distilled water is formed by condensation of water vapor.

However, in fact, because the distillation device and receiving device cannot be 100% clean, and the microorganisms in the air may also fall into the water after being placed, there are microorganisms in the distilled water we usually see.

Pure water prepared by the distillation method. Distilled water can be divided into single and multiple times. After a distillation of water, the non-volatile components (salts) remain in the container and the volatile components (ammonia, carbon dioxide, organics) enter the initial fraction of the distilled water, usually only the middle part of the fraction is collected, which accounts for about 60.

To obtain purer water, alkaline potassium permanganate solution can be added to primary distilled water to remove organic matter and carbon dioxide; A non-volatile acid (sulfuric acid or phosphoric acid) is added to make ammonia a non-volatile ammonium salt. Since glass contains a small amount of water-soluble components, it is necessary to use quartz distillation vessels to obtain very pure water when performing secondary or multiple distillations, and the resulting pure water should be kept in quartz or silver containers.

No, because microorganisms survive by breaking down organic matter, and the organic matter in distilled water is volatilized.

No. There are only water molecules, nothing else.

No. Distilled water is water that has been evaporated and condensed by ordinary water, which is absolutely pure. Free of impurities, including microorganisms.

First of all, it is necessary to clarify the concept that distilled water is condensate after evaporation. Therefore, distillation mainly removes non-volatile substances (which do not leave the distillation flask with water vapor), like some organic liquids that are slightly soluble in water (such as ethyl acetate), which will still be present in small parts after distillation.

It is entirely possible for some heat-resistant microorganisms (such as Hydrobacter thermalis) to enter the condenser tube with water vapor, but these microorganisms are rare. So the most common situation is that the microorganisms in the air enter the water when the condensate is collected. But since distilled water has almost no nutrients, it does not multiply in large quantities.

Hence it can be assumed that there are no microorganisms in distilled water. (Except for the virus that is resistant to high temperatures, but it seems that there are none.) )

Direct microscopic observation and scribing culture can be done...

Test Topic: Determination of the Quantity of a Microbial Preparation and Application of Solid-Phase Enzyme DNA for Crude Extraction and Identification Special Topic: Analysis:

When answering, pay attention to whether distilled water or sterile water is used in the experiment, and dilute the E. coli culture medium in a series of macro key gradients to prevent contamination by miscellaneous bacteria, and sterile water must be used; Chicken blood cells were broken to obtain filtrate containing DNA with distilled water; Solid media for the preparation of E. coli culture can be sterilized with distilled water and then autoclaved; When preparing immobilized yeast cells, activate dry yeast with distilled water a. Isolate: Carry out serial serial serial dilution of E. coli culture medium to prevent contamination by miscellaneous bacteria, sterile water must be used, a correct; B. Distilled water is used to obtain filtrate containing DNA by crushing chicken blood cells, B is wrong; c. The solid medium for the preparation of culture of E. coli can be sterilized with distilled water and then autoclaved, c error; d. Distilled water is used to activate dry yeast during the preparation of immobilized yeast cells, d error Therefore, a Comments: This question examines the use of materials in experimental operations, and examines students' experimental skills and application abilities, and the difficulty of the test questions is medium