What is a T vector and what is a T vector? What are the types of T vectors? What s the difference

The plasmid PMD-18TMAN2XCMI was constructed by cloning the mannanase gene into the PMD-18T vector, and a specific XCMI cleavage site was introduced on each side of the cloned mannanase gene. T vectors can be prepared by digesting plasmid PMD-18TMAN with XCMI and 2XCMI. Because the mannanase gene is controlled by the galactosidase gene promoter as a filler fragment and selection marker, mannanase is able to be expressed, thereby hydrolyzing mannan to produce a hydrolysis circle that indicates the background colony of a partially digested plasmid. In addition, there is a 3rd XCMI cleavage site with a different sequence in the mannanase gene, so the background caused by the residual mannanase gene can be further reduced.

A large number of plasmids were extracted and stored at any time to prepare T vectors for use, which increased the stability of cloning. This T vector overcomes the shortcomings of general T vector with unstable quality, cost, low cloning efficiency and high false positives. Ten genes were cloned using the constructed T vector, and the results showed that the recombination efficiency reached 90 100

It is a vector modified with a T virus, so that the gene of the T virus carries a certain gene fragment of interest, and then introduces it into the recipient cell.

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The t-vector is also known as the bait-vector. Polymerase chain anti-response product clonal transport vector, after linearization.

Each of the 3 ends of DAO on both sides of Zhi has an extra deoxythymidylate (T). Since PCR products usually contain a single deoxyadenylate (A) at the 3rd end of the two-accommodating side, the A-T complementarity with the T vector can improve the cloning efficiency of PCR products.

The T vector is a cloning vector, that is, a vector used to amplify the gene of interest in recipient cells, which cannot be expressed in large quantities. It is mostly used to clone PCR products, and is generally modified with some common vectors, and the general DNA polymerase does not have 3'-5'Exonuclease activity, i.e., the corrective activity of the enzyme, is found in PCR product 3'The end is added with an adenylate residue (A) in a non-template-dependent manner, so that the PCR product is 3'Adenylic acid can be compared with T-carrier3'Thymine complements each other, and it is this base-pairing that underlies the efficient ligation of the T-carrier system. Conversely, a polymerase with corrective activity will remove this3'Overhangs, resulting in blunt-ended PCR products that cannot be cloned directly with the T-vector system.

If you want to express a large number of PCR amplified gene products, the common method is to first connect the PCR product into the T vector for amplification (the T vector used needs to be designed with the enzyme digestion site in advance), and then use the corresponding enzyme to cut the target fragment, and then connect the expression vector with the same enzyme digestion to express the protein in large quantities.