What is needed for a lily plant tissue culture experiment

The medium, the sterilizer, the lily are not heat-resistant and require air conditioning.

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How to cultivate lilies:

1. Environment. Lilies like a warm, moist and sunny environment, slightly tolerant to semi-shade and cold, intolerant to high temperatures, afraid of waterlogging, the growth temperature is 15 25 degrees, the temperature is higher than 30 degrees and below 10 degrees, the growth is slow or dormant.

2. Soil. Baibu Liang Luhua likes loose key old fertile, well-drained slightly acidic sandy loam, potting soil can be mixed with humus, garden soil, river sand, planting pot bottom pad granular stone, add a little organic fertilizer as a substrate.

3. Watering. Lily peanuts keep the soil moist for a long time, the soil is moist and will cause root rot, when it comes to the flowering period, the water should be sufficient, reduce watering after flowering, stop watering after the aboveground part of the plant withers, and keep the potting soil slightly dry.

4. Fertilization. Lily peanuts are applied with nitrogen, phosphorus and potassium water and fertilizer once a month for a long time to promote the vigorous growth of plants, and the irrigation of potassium dihydrogen phosphate 1:1000 during the flowering period is conducive to colorful flowers!

5. Rizhao. Lilies are short-day plants that prefer semi-shade, but they need to be sunny during the growing season, and growing in a shady environment for a long time will cause less flowering or flower buds to fall off. Potted lilies have sufficient sunshine, the plants grow strongly, the flowers are bright, and it is better to have no less than 6 hours of sunshine a day, otherwise flowering will be delayed.

6. Management. Lilies should be tied up in front of the buds to prevent lodging, in the high temperature and humid season will be sick and pests, the soil of cultivated leaves should be disinfected, remember to water the soil is too wet, otherwise it is easy to get sick, pay attention to prevention.

1. Land preparation planting: lily is cold-resistant, likes semi-shady environment, and avoids drought. Before planting, the plot should be selected, enough fertilizer should be applied, enough water should be irrigated, the land should be prepared, and the soil should be treated with soil insecticide and weeding.

Lily generally take the method of dividing ball propagation, scale cuttings, sowing propagation and tissue culture for propagation, no matter what way is taken, it is necessary to spray the seeds (seed pieces) with the new high lipid film 800 times, which can effectively repel underground diseases and insects, isolate virus infection, do not affect the germination and swelling function, strengthen the breathing intensity, and Hu Liangchen improves the seed germination rate.

2. Seedling management: After the emergence of lilies, it is necessary to cultivate and weed in a timely manner, loosen the soil, coarse underground forest and healthy development of roots, and spray new high-fat film spray on the surface of the ground, which can protect moisture and fertilizer efficiency, prevent evaporation and transpiration, suffocate and repel insects and prevent weed migration.

3. Flowering control: The lily leaves are emerald green, the flower shape is peculiar, the color is elegant, and it is very lovely. Spraying flowers at the bud stage can promote strong buds, hypertrophic petals, bright flower color, rich floral fragrance, and prolonged flowering period.

At the same time, the lily is planted and harvested by the bulb, and the fruit should be sprayed before the flowering period to make the nutrient transport duct thicker, improve the bulb expansion and vitality, smooth fruit surface, strong fruit shape, high quality and high yield.

4. Pest control: there are many pests and diseases of lily, and the most common diseases are soft key rot, rot disease, mosaic disease, and anthracnose; Insect pests include root ticks and grubs. It is necessary to carry out comprehensive prevention and control in accordance with the principle of disease treatment, insect extermination, and prevention if there is none, spray targeted drugs to kill pests and diseases during the pest and disease hazards, and add a new high-fat film to improve the utilization rate of pesticide active ingredients, suffocate and isolate the source of pests and diseases, protect the plant to thrive, make the flowers bloom more brightly, and the fruit grows stronger.

Tissue culture propagation:

1.Selection of explants Qitong.

Healthy bulbs that have undergone low temperature treatment are usually used, and robust scales (or young flower buds in the production stage) near the outside and in the middle are selected as explants.

2.Culture medium.

ls plus milligrams. MS plus milligram liter plus milligram liter can be. The musk lily Snow Queen cultivar also produces a large number of daughter bulbs under culture conditions of induction medium (ms plus 6-ba2 mg plus mg wheel collapse liters).

3.Cultivation conditions.

Incubated at 30 h in 16 hours of sunlight at a light intensity of 1200 lux. After 6 weeks of incubation, more than 8,000 new spheroids can be formed directly on 100 cultured scales. Before planting, the daughter bulb was treated at low temperature for 2 weeks 4 weeks, and after transplanting, the leaves and roots on the daughter ball scales were good.

1. Planting time

Lilies are suitable for planting in September and October in autumn, when the temperature is just stable at around 15 degrees Celsius, which is very conducive to germination and growth, which is helpful for later growth.

2. Prepare the bulbs

The right bulb is an important foundation for growth, which requires fullness, fullness, and no bumps on the outside. After selecting the bulbs, they should be disinfected, soaked in carbendazim solution, removed and cleaned after about 20 minutes, and then planted after drying.

3. Potting soil selection

Its roots are relatively developed, and it is necessary to choose a deep pot, about 20 cm in diameter. The soil should have the characteristics of good drainage and good air permeability, and can be mixed with peat, river sand, and perlite in a ratio of 2:2:1, and a little base fertilizer can be added, and clay must not be used.

Fourth, plant bulbs

Put 10-15 cm of soil in the prepared pot, then put the bulbs in it, the scales should be opened upwards, cover it with about 5 cm of soil, and water it thoroughly.

Fifth, post-maintenance

When the bulbs are planted, you can put the potted plants in the shade for 1-2 days, and then put them in a scattered place to adapt, do not contact strong sunlight first, keep the shading at about 60%, and wait until the growth is stable and contact with the sun.

Let's cultivate it carefully! Don't get too much sun in the summer and move in in the winter! The next year will grow new shoots from dry roots!

1.It is best to disinfect before planting lily bulbs, I generally soak it in chlorothalonil or methyl tobuzine 600 times solution for 20 minutes, rinse with water, and dry it before planting. Fill the bottom of the pot with 2-3 cm of soil and place the ball, if it is a pot with three heads, the head of the ball is placed towards the edge of the pot, that is, like the edge of the pot, which can ensure that the plants are evenly distributed after germination.

The top of the ball should be covered with 10 cm of soil, and the minimum should not be less than 5 cm. After planting, water thoroughly and put it in a cool place to take root.

2.To keep it moist is to grab a handful of soil with your hands and pinch it vigorously, so that water seeps out of your fingers but does not drip down. Don't overwater, too much water is easy to rot the roots, watering it thoroughly, not just half of the water.

Tap water can only be used after a day or two. The air humidity should reach 60-80%, and the humidity should be stable, if the humidity difference is too large, it is easy to get leaf burn, especially during the period from the beginning of the flower bud to the flowering. At home, you don't need to bother so much, just spray water twice in the morning and evening.

I usually blow it with a humidifier. ）

3.Fertilization: The amount of fertilizer for lilies is not large, if the family is planted and played, it is generally fertilized once every half a month, I use bottled liquid fertilizer, if the conditions allow to dig the bend, it is better to use organic fertilizer.

The book is more complicated, to apply foliar fertilizer before the 22nd day after germination, with urea and potassium dihydrogen phosphate, after 22 days to leach water fertilizer as the main judgment, once every 3-5 days, with potassium nitrate, magnesium sulfate, boric acid, potassium nitrate, potassium dihydrogen phosphate in the bud expansion period, etc., I think it is too troublesome, or use liquid fertilizer to save trouble).