What is the experimental principle, preparation and operation procedure of sterility test for biolog

How to ensure sterility when handling microorganisms in the laboratory.

Hello, dear. The following points need to be done to ensure that there is no lead bacteria in the shirt: When inoculating the bacteria, you must wear work clothes and wear a work preparation hat; When inoculating food samples, special work clothes, caps and slippers must be worn, which should be placed in the buffer room of the sterile room and used after ultraviolet disinfection before work; When inoculating food samples, wash your hands with soap before entering the sterile room, and then wipe your hands with an alcohol cotton ball; Straws, plates and culture media used for inoculation must be sterilized and sterilized, unpacked unused utensils cannot be placed before use, and metal utensils should be autoclaved or burned three times with alcohol before use; When removing the straw from the package or well, the tip of the straw should not touch the edge of the tube or dish; When inoculating live bacterial samples, the straw must be sterilized by flame after removing the package and opening the test tube stopper; The inoculation ring and needle should be burned by flame before inoculating bacteria, and if necessary, burned to the connection between the needle and ring and the rod, and the inoculation ring for inoculating tuberculosis bacteria and virulent bacteria should be boiled in boiling water for 5min, and then cauterized by flame; When sucking the bacterial liquid or sample with a pipette, it should be sucked with the corresponding rubber tip, and should not be directly sucked by mouth.

The aseptic operation of microbiological experiments can prevent microorganisms from entering human tissues or other sterile areas of growth and reproduction, which can affect the conduct of the experiment. Aseptic operation is an important basic operation in various biological experiments and production practices. The requirements for aseptic operation are:

Kill microorganisms such as bacteria and viruses in the operation space before operation; During the operation, the operating space is isolated from the outside world to avoid the invasion of microorganisms.

1. The aseptic operation itself ensures the authenticity of the sample and avoids the contamination of the sample.

2. Aseptic operation also includes the requirements for the operator, so it also protects the safety of the operator itself.

3. Aseptic operation is generally carried out in a specific biological safety cabinet, which also makes use of the characteristics of the biological safety cabinet to ensure that the aerosols formed during the operation will not spread to the environment, so it is also a protection for the environment.

To achieve triple protection, 1. The aseptic operation itself ensures the sample.

authenticity to avoid contamination of the sample; 2. Aseptic operation also includes the requirements for the operator, so it also protects the safety of the operator itself. 3. Aseptic operation is generally carried out in a specific biological safety cabinet, which also makes use of the characteristics of the biological safety cabinet to ensure that the aerosols formed during the operation will not spread to the environment, so it is also a protection for the environment.

Ensure that the experimental results are not disturbed by miscellaneous bacteria, and at the same time ensure your own safety.

The ones that should be prepared by aseptic manipulation are: powder needles.

Aseptic operation refers to an operation method in which the entire process is controlled under aseptic conditions. This method is suitable for the preparation of injections, ophthalmic preparations, skin test solutions, sponges and traumatic agents of some heat-resistant drugs. Products prepared according to the aseptic operation method are generally no longer sterilized.

In order to ensure its sterility, special (heat-resistant) varieties can also be resterilized (e.g. penicillin G, etc.). The production process of the final sterilized product is generally to avoid bacteria (try to avoid microbial contamination), such as the preparation of most injections.

The use of sterile holding forceps The sterile holding forceps are soaked in a large-mouth container containing disinfectant, the bottom of the container is padded with sterile gauze, and the liquid level is immersed 2 3cm above the shaft joint, or 1 2 of the tweezers, and only one sterile holding forceps can be placed in each container. Sterile forceps and their soaking containers should be cleaned and disinfected weekly, and disinfectant and gauze should be replaced, and departments that use more should be cleaned and disinfected daily.

When taking and placing the sterile holding forceps, the ends of the forceps should be closed, and the inner wall of the container above the mouth edge of the container and the solution surface should not be touched when taking out. Keep the end of the pliers downward when using, and do not turn upwards in reverse; Return to container immediately after use. Pick up the item at a distance, and the sterile forceps should be removed along with the container.

Sterile forceps should not touch unsterilized items, and should not be used to pick up oil gauze, change dressings, and disinfect**.

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The key points in the process of testing microbial samples are as follows. Each time the medium is configured, it is necessary to pay attention to whether the production date is within the shelf life, check the opening date and the sealing of the bottle to ensure that it has not deteriorated and has not absorbed moisture<>

Key points in the process of sample testing for microbial experiments.

Dear, I'm glad to answer your <>

The key points of operation in the process of microbial experimental sample testing are as follows. Every time you configure the medium, you should pay attention to whether the production date is within the shelf life, check the opening date and the bottle mouth seal to ensure that it has not deteriorated and has not absorbed moisture<>

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When preparing the medium, it is important to weigh accurately, including the dispensing of saline. It is necessary to ensure that the prepared medium is now sterilized, and the unsterilized prepared medium cannot be placed for a long time, let alone overnight. When sterilizing the medium, it is necessary to ensure constant temperature and pressure, and at the same time to ensure the effective sterilization time.

The sterilized medium should be stored in the refrigerator, which can be stored for a week, generally not more than 3 days. Moreover, it is necessary to follow the principle of "first-in, first-out", and the medium prepared by the first-to-sell faction should be used first.

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When using, according to the amount of use of the shift, the medium should be dissolved into a liquid state in a water bath, and then the temperature of the water area should be lowered in time, and the first can be taken out from the water area, and placed on the lid of the water bath for later use, and the medium must not be kept in a high temperature state for a long time. When doing the microbial testing of the product, the temperature of the pouring medium should be about 45, not too hot, and the fungus should not be scalded. It should not be too cool, and the melted medium will be agglomerated and solidified, which is not convenient for observing the results. Each bottle of culture medium should be blank and try to concentrate on Chunxin as much as possible to avoid frequently opening the cork of the same bottle of culture medium, increasing the probability of staining of the culture base oak, and the error results will occur.

If there is too little medium left, you can pour it into a blank plate first. <>

Summary. It is determined by the relevant enzyme classes of bacteria and fungi. Most yeasts are obligate aerobes, so their experimental design is mostly based on sugar. The difference between a full cabinet and a clean bench is the direction of the wind: the wind direction of the biosafety cabinet is first vertically downwards and then towards the cabinet.

What are the wrong biosecurity requirements regarding fungal testing operations? Most yeasts can be used on an open bench.

It is determined by the enzymes associated with bacteria and fungi. Most yeasts are obligate aerobic bacteria, so their experimental design is mostly based on sugar. The difference between the whole cabinet and the clean workbench is the direction of the wind: the wind direction of the biosafety cabinet is to first go vertically down and then transport it to the cabinet.

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How to ensure aseptic operation in microbiological testing operations? Anhui Renhe Purification introduces you to the aseptic operation requirements for microbial testing

The main reason for the staff of the microbiology laboratory is to prevent the artificial contamination of the samples during the test operation, and the second is to ensure the safety of the staff and prevent the detected pathogenic bacteria from causing personal contamination due to improper operation. The requirements are as follows:

When inoculating the bacteria, you must wear work clothes and wear a work cap;

When inoculating food samples, special work clothes, caps and slippers must be worn, which should be placed in the buffer room of the sterile room and used after ultraviolet disinfection before work;

When inoculating food samples, wash your hands with soap before entering the sterile room, and then wipe your hands with an alcohol cotton ball;

Straws, plates and culture media used for inoculation must be sterilized and sterilized, unpacked unused utensils cannot be placed before use, and metal utensils should be autoclaved or burned three times with alcohol before use;

When removing the straw from the package, the tip of the straw should not touch the edge of the tube or dish;

When inoculating live bacterial samples, the straw must be sterilized by flame after removing the package and opening the test tube stopper;

The inoculation ring and needle should be burned by flame before inoculating bacteria, and if necessary, burned to the connection between the needle and ring and the rod, and the inoculation ring for inoculating tuberculosis bacteria and virulent bacteria should be boiled in boiling water for 5min, and then cauterized by flame;

When sucking the bacterial liquid or sample with a pipette, it should be sucked with the corresponding rubber tip, and should not be directly sucked by mouth.