How does the clean bench work and how to use it?

Shanghai Bajiu] reminds you:

First, the working principle of the ultra-clean workbench.

The ultra-clean workbench cage box is made of transparent plastic material that is resistant to high temperature, and a set of cage box is composed of an upper cover, a bottom box, a trough, a sink, a locking fastening, an air inlet and outlet assembly, a silicone rubber sealing gasket, etc., and an air filter called the window of life is also on the upper cover. The independent ventilation cage box is the key to IVC equipment, which should have a certain airtightness, prevent the entry of air outside the box to reduce possible infection**, and allow clean air to enter smoothly, and form a good air flow or diffusion in the box, mix with the gas in the box and discharge the exhaust gas in the cage. There are generally two basic forms of cage boxes, and most of them are patented.

One is the ventilation and exhaust of the cage box is the valve opening and closing type, that is, it is opened when it is put on the shelf and automatically closed when it is removed. Valves include camera shutter type and monolithic spring switch type. The other is the in-box terminal filter protection type.

There are two types of filter membranes: cylindrical and plate. Most of the filter membranes are made of sub-high efficiency or high efficiency filter materials.

Second, the use of ultra-clean workbench steps.

1. Preparation before the operation and use of the ultra-clean bench.

1) Turn on the ultra-clean working fan to purify the environment in the station. Turn on the work light to reach the working illumination.

2) Check whether the various sterilization feeding and experimental items prepared in the ultra-clean bench are complete. If the cage needs to be replaced. Check the readiness of the sterilization cage box and move it to the sliding door of the clean table with a stainless steel cart.

3) Check whether the spraying effect of the sprayer and the liquid used for wiping inside and outside the ultra-clean table have been configured.

4) Turn on the ventilation system in the room. Exclude odorous gases escaping when the cage is opened.

5) Check the pointers of various meters of the IVC unit and visually inspect the animals in each cage box to estimate the situation of the IVC operation.

6) Record the main environmental parameters that affect the reproduction, growth and development of experimental animals, such as temperature, relative humidity, daily temperature difference, pressure difference between inside and outside the cage, etc.

2. Procedures and specifications for the operation and use of ultra-clean workbenches.

1) Personnel should wear disposable masks, hats and medical latex gloves.

2) Gently lift the outer end of the IVC cage box with both hands, move the cage box outward along the cage rack shelf, and place it on the stainless steel trolley next to the ultra-clean workbench.

3) Spray the surface of the gloves and the outside of the IVC cage box with a liquid medicine sprayer, disinfect and sterilize and stick the dust on the surface to prevent laminar gas blowing when entering the ultra-clean table. You can also use gloves to dip your hands into the liquid medicine container, pick up and wring out the liquid medicine towel, dry the droplets outside the gloves, and wipe the surface of the cage box at the same time.

4) Open the sliding door of the ultra-clean workbench and move the cage box into the ultra-clean workbench.

5) Pull down the sliding door of the ultra-clean workbench appropriately. Open the fastening clasp between the bottom of the IVC cage box lid and open the cage lid. Lay on your side and put it aside.

The use of the ultra-clean workbench and precautions are as follows:

1. Prepare before starting.

Check the status mark, the equipment should be in good condition. Turn on the ultraviolet lamp 30 minutes before use, and irradiate and sterilize the working area. 20 minutes before use, start the purifying air and wipe the first side of the Tableaus macro clean.

Wipe and disinfect the surface of the tools that need to be used and put them on the workbench after wiping and disinfecting them. Pull down the glass covers on both sides of the workbench. Pay attention to the pressure of both hands evenly.

2. Turn on. Turn on the light switch while operating and turn off the UV lamp at the same time. The operation area is a laminar flow area, so the number of working beams should not hinder the normal flow of airflow, and the staff should avoid actions that can cause disturbance of airflow. The operator should wear work clothes.

Ultra-clean workbench

The clean bench is designed to meet the needs of modern industry, optoelectronic industry, biopharmaceutical, scientific research and testing and other fields for the cleanliness of local work areas. Note: A clean bench is not the same as a biosafety cabinet.

The ultra-clean workbench can only protect the reagents and other reagents operating in the workbench from contamination, and does not protect the staff, while the biological safety cabinet is a negative pressure system, which can effectively protect the staff. Ultra-clean workbench: Ultra-clean workbench is a kind of local purification equipment, that is, the use of air cleaning technology to make the space in a certain operation area to achieve a relatively dust-free and sterile state.

What is the correct way to use the clean bench or how to operate it? Renhe Purification introduces you to the instructions for the correct use of ultra-clean workbenches.

1.Turn on the power supply of the ultra-clean workbench, turn off the ultraviolet lamp, and wipe and disinfect the worktop with 75% alcohol or peracetic acid.

2.Each time the clean bench is used, the experimenter should first turn on the ultraviolet lamp on the clean bench and use it after 20 minutes of ultraviolet irradiation.

3.During the whole experimental process, the experimenter should operate in accordance with the aseptic operation protocol.

4.In case of unit failure, the laboratory animal room should be notified immediately, and the professional personnel will continue to use it after passing the inspection.

5.After the experiment, wipe the work surface with disinfectant, turn off the working power, and turn on the UV lamp again for 15 minutes6

The filter material of the ultra-clean workbench should be replaced every 2 years, and the replacement record should be made7

Experimenters should pay attention to keeping the room clean and tidy.

The above is provided by Anhui Renhe Purification, if you have any questions, you can view, or ask 13156661116 4008-252627

To put it simply, it is to use sterile and dust-free air to form an air barrier to protect the experimental samples. To be more detailed: the ambient air passes through the primary filter, and after being sucted into the fan, it passes through the high-efficiency filter under pressure to form a sterile and dust-free clean air, and then supplies air to the working area.

Because there is clean air inside the workbench, the pressure in the working area is slightly greater than that of the external environment, so the ambient air will not enter the working area, so as to ensure that the entire working area is sterile and dust-free.