How much does it cost to do a clean workbench for microbial culture

The ** of single and double is not the same, and the ** of vertical flow and horizontal flow are not the same.

1. How to make it:

Isolation and culture of microorganisms are inseparable from solid media. In microbiology labs, the use of solid media is so frequent and routine that this method seems like a no-brainer. However, back in 1881, before the advent of solid media, the cultivation of microorganisms could only be carried out in liquid media.

In order to be able to directly observe the morphology and growth of the culture, scientists want to grow microorganisms on solid surfaces, just as microorganisms grow on orange peels or potatoes. Robert Koch (1843-1910), a German physician, used boiled and sterilized potatoes to grow bacteria. After that, he experimented with using gelatin as a coagulant for the medium.

He added gelatin to a liquid medium to thaw it, and then slowly poured the evenly mixed liquid onto the surface of a glass plate. When the gelatin cools and solidifies, a solid medium forms on the surface of the glass plate. To prevent contamination by germs in the air, Koch also used a glass cover to isolate the glass panes from the surrounding environment.

However, it was soon discovered that gelatin softened at more than 20 years old, making it difficult to strip to isolate microorganisms. Gelatin liquefies at temperatures above 25, and most bacteria are incubated at temperatures below 25.

2. Definition of Petri dish:

A petri dish is a type of labware used for microbial or cell culture that consists of a flat disc-shaped bottom and a lid, generally made of glass or plastic. Petri dish materials are basically divided into two categories, mainly plastic and glass, glass can be used for plant material, microbial culture and adherent culture of animal cells may also be used. The plastic may be made of polyethylene material, which is available in disposable and multi-use applications, and is suitable for laboratory inoculation, scribing, and isolation of bacteria, and can be used for the cultivation of plant material.

Microbiological experiments involve a variety of level requirements, and the "ultra-clean workbench" can provide local dust-free and sterile working environment air purification equipment, which is not a corresponding equipment due to different users. For ordinary microbiological experiments that generally do not require in a sterile and dust-free environment, even if experts do it, it is not necessary to use an ultra-clean workbench.

The whole cabinet of biological security can not only protect drugs, but also protect people and the environment, but the ultra-clean platform can only focus on Li to protect drugs. So from a theoretical point of view, it can be replaced, but ** can be blocked several times.

The main differences between a clean bench and a biosafety cabinet are as follows:

1. A biosafety cabinet is a cabinet that absorbs air and prevents bacteria or reagents from splashing out and contaminating laboratories and laboratories. It is mainly used to protect the human body.

2. The ultra-clean workbench is an instrument that ensures the sterile environment of the test bench without considering the laboratory and experimenters.

3. Biological safety cabinet is a kind of safety equipment widely used in research, teaching, clinical testing and production in the fields of microbiology, biomedicine, genetic recombination, animal experiments, biological products, etc., and is also the most basic safety protection equipment at the national level. Laboratory biosafety protective barriers.

4. The biological safety cabinet is a kind of negative pressure purification workbench. Proper operation of the biosafety cabinet can completely protect staff, samples, and prevent cross-contamination; The clean bench is just a clean bench that protects the object of operation, not the staff and the laboratory environment. Therefore, in microbiology and biomedical research, teaching, clinical trials, and manufacturing, biological safety cabinets should be used instead of ultra-clean benches.

The biosafety cabinet is designed to protect the staff, the laboratory environment, and the experimental objects from exposure to infectious aerosols and spills that may be generated during the manipulation of infectious experimental materials such as original cultures, bacterial strains, and diagnostic specimens.

Ventilation cabinets (vent hoods) are designed to remove corrosive chemical gases and toxic fumes during chemical experiments. Since it is not equipped with a HEPA filter, the vent hood cannot effectively remove microbial media. Microbial samples placed in a ventilated cabinet can spread outside the cabinet and contaminate the laboratory environment.

Clean benches are designed to protect test specimens or products by blowing vertical or horizontal laminar air through the work area to prevent contamination of the test piece or product by dust or bacteria outside the work area. Once the microbial sample is placed in the work area, the laminar flow of air will blow the air with the microbial medium to the front desk staff and pose a hazard.

Suzhou Hisiyuan.

Locally, it is basically 100 grades; Microparticles do not exceed 100 per square foot; The number of colonies is in the state of the ultra-clean workbench open, take a number of non-missing number guess petri dishes with an inner diameter of 90mm, inject about 15ml of nutrient agar medium that is melted and cooled to about 45 in aseptic operation, put it until it is solidified, and invert it in the 30 35 incubator for 48 hours, after proving sterility, take 3 5 plates, and place them in the left, middle and right places of the working position, after opening the lid and exposing it for 30 minutes, invert it in the 30 35 incubator for 48 hours, take out and check, The average number of miscellaneous bacteria in the plate in the 100 clean area shall not exceed 1 colony. Volt type.

There are a large number of microorganisms in the air in our daily environment, which are usually floating in the air along with dust and fine particulate matter in the air. For pharmaceutical, food and bioengineering production, the production process or product has strict microbiological requirementsThe production environment is an important part of microbial contamination and control。Therefore, the number of microorganisms in the environment must be strictly controlled in this type of production plant, usually by sealing the production hall and strictly filtering the internal air to achieve the required cleanliness.

Regular microbial testing of the air and workbench in the production environment is to check whether the microbial control requirements in the production process are met and whether the requirements of the relevant standards and specifications are met by checking the microbial level (quantity) of the production environment, and it is also a means and method to check whether the production environment is sealed and whether the internal air filtration system is operating effectively.

The limit room is mainly used for limit inspection, that is, the specified amount of samples contains the amount of dust bacteria, which is to provide a relatively clean environment for testing.

There is no requirement to wear clean clothes when opening the clean workbench, but theoretically speaking, it would be better to wear them!

Previously, the icleanbench workbench used in the first workshop of the North China Pharmaceutical Factory had been stipulated, and the staff had to do a good job of protection in order to work.

Biological safety cabinets and ultra-clean benches are widely used in microbiology, animal experiments, biological products and other fields, but there are differences between the two:

The biological safety cabinet is used to protect the operator, the laboratory environment and the experimental materials, and the operation area is under negative pressure, which can prevent biological germs or reagents from splashing out of the safety cabinet to pollute the laboratory and the operator; The clean bench only protects the sample, not the operator. Therefore, in order to protect the operator and the environment, it is best to carry out these experiments in a biological safety cabinet without knowing whether microorganisms are harmful to humans.

Microbiology laboratories should generally choose biological safety cabinets, which are negative pressure, and the wind will not blow on the experimenter, which is safe. If it is a non-harmful microorganism, it is still necessary to choose vertical air supply, because the horizontal air supply wind will blow directly to the operator in a large area.

Shakers, incubators, refrigerators, clean benches, centrifuges, sterilizers, etc. The wind in our laboratory clean table blows from top to bottom, I don't know which one is better, it should be blown outside.

Incubators, sterilizers, microscopes, refrigerators, and preferably a pathogen identification system would be better.

Our laboratory is vertical, I haven't used parallel air supply, I think the vertical one is quite easy to use.

It depends on the experimental requirements, there are many microbial experiments, and the specific direction to do is different, and the equipment required is different.

Ultra-clean workbench is generally used for vertical flow air supply, depending on your specific experimental needs.