Seawater formulations for bacterial media, bacterial medium formulations

1. Bacterial culture medium.

Recipe 1: Beef paste agar medium.

Beef paste grams, peptone grams, sodium chloride grams, agar grams, water 100 ml.

Add 100ml of water to the beaker, add beef paste, peptone and sodium chloride, mark the outside of the beaker with a crayon, and then put it on the fire to heat. After the components in the beaker are dissolved, add agar and stir constantly to avoid sticking to the bottom. After the agar is completely dissolved, make up for the water loss, adjust the pH value with 10% hydrochloric acid or 10% sodium hydroxide, divide it into each test tube, add cotton stopper, and sterilize with autoclave for 30 minutes.

Recipe 2: Potato medium.

Take 250 grams of fresh beef heart (remove fat and blood vessels), finely chop it into minced meat with a knife, add 500 ml of distilled water and 5 grams of peptone. Mark a beaker, bring to a boil, and simmer for 2 hours. Filtration, the filtered minced meat is dried, and the pH value of the filtrate is adjusted to about it.

Add 10 ml of broth and a small amount of minced dried beef heart to each tube, sterilize and set aside.

Recipe 3 rhizobia medium.

Glucose 10 g dipotassium phosphate grams.

Calcium carbonate 3 grams magnesium sulfate grams.

Yeast powder g agar 20 g.

Water 1000 ml 1% crystal violet solution 1 ml.

First, the agar is boiled and dissolved in water, and then other components are added separately, stirred to dissolve, and then packaged, sterilized, and set aside.

2. Actinomycete medium.

Recipe 1: Starch agar medium (Gao's medium).

Soluble starch 2 g potassium nitrate g.

Dipotassium phosphate grams of sodium chloride grams.

Magnesium sulfate grams, ferrous sulfate grams.

Agar 2 g water 100 ml.

First, put the starch in a beaker, mix it into a paste with 5 ml of water, pour in 95 ml of water, stir well, and add other medicines to dissolve it. Make a mark on the outside of the beaker, add agar when it is heated to boiling, stir constantly, and make up for the water loss after the agar is completely dissolved. Adjust the pH value, sterilize after aliquoting, and set aside.

Recipe 2 Flour agar medium.

60 grams of flour 20 grams of agar.

Water 1000 ml.

Mix the flour into a paste with water, add water to 500ml, and simmer for 30 minutes. Take another 500 ml of water, put in agar, heat and boil until dissolved, mix the two liquids thoroughly, add water, adjust the pH value, divide the package, sterilize, and set aside.

3. Fungal culture medium.

Recipe 1: Sabouraud's medium.

Peptone 10 g Agar 20 g.

Maltose 40 g water 1000 ml.

After adding water to peptone and agar, heat it, stir it constantly, and after the agar is dissolved, add 40 grams of maltose (or glucose), stir it to dissolve, and then divide it, sterilize and set aside.

This culture is commonly used to culture many species of fungi.

Microbiology Experiments Preparation of bacterial culture medium (special for the new coronavirus pneumonia epidemic).

Grow photosynthetic <> with seawater

When bacteria, it is necessary to add an appropriate amount of peptone as a nutrient source. The specific amount of peptone to be added needs to be determined according to different strains, medium formulations and other factors. Generally speaking, the amount of peptone should be added to ensure that the concentration of nitrogen in the bacterial solution is in between.

Suppose you need to use 1L of seawater medium to cultivate photosynthetic bacteria, requiring the addition of peptone, and the nitrogen concentration is, then according to the composition and molecular weight of peptone, the following calculation formula can be obtained: n (peptone mass, unit: gram) = 1L (medium volume) Nitrogen concentration, unit:

Percentage) 140 (peptone molecular weight) Substituting the above values into the formula, we get: n = 1 140 = unit: grams) Therefore, you need to add grams of peptone to 1 L of seawater to get the desired nitrogen concentration.

It should be noted that in the process of adding peptone, it is best to use aseptic technique to avoid contamination.

Yes. In recent years, with the support of policies, the development momentum of China's biological products industry has been strong, capital investment has continued to increase, and import substitution has gradually been realized in some product fields. As a key raw material for biological products, driven by the rapid development of the biological products industry, the scale of the cell culture media industry continues to expand.

According to the "2021-2025 China Cell Culture Media Industry Market Monitoring and Future Development Prospect Research Report" released by Xinsijie, in 2020, the scale of China's cell culture media market reached more than 4 billion yuan. However, most of China's cell culture media products rely on imports, which has adversely affected the healthy development of China's biological products industry.

The high dependence of cell culture media products on foreign countries is mainly due to the high technical barriers of the product, the late start in China, and the lack of R&D experience, resulting in the slow process of product development. The preparation and application of cell culture medium involves biology, chemistry, physics, medicine and other disciplines and cutting-edge technology, and the formula generally contains 70-100 different chemical components (including sugars, amino acids, vitamins, inorganic salts, trace elements, growth-promoting factors, etc.), which need to be determined by analyzing cell characteristics and process tests to determine the formula components suitable for cell growth, which often requires repeated and large number of experimental demonstrations and scientific analysis.

And users in different application fields have a very strong personalized demand for the formulation of cell culture media, which leads to the research and development of cell culture media products need to invest a lot of manpower, material resources, financial resources and time, so although in recent years, the number of enterprises and Yunfan research institutions in this field has continued to grow, but the large-scale production is very rare

Marine microbial culture media generally require a certain amount of seawater salt, while terrestrial microbial culture media do not.

1) Broth liquid medium (solid medium component, without agar is liquid):

1. Ingredients: beef paste, peptone, sodium chloride, 100ml of distilled water.

2. Preparation method: heat and dissolve to dissolve, adjust the pH value of the previous area, divide it into a triangular bottle for 121, and autoclave it for 20min.

2) The formulation of LB medium is as follows:

Peptone (tryptone).

10 g l yeast extract (yeast.)

extract)

5 g l sodium chloride (NaCl).

10 g l dissolved in distilled water in proportion, packed into an Erlenmeyer flask, sealed with gauze + newspaper or kraft paper, and sterilized by high repentant pressure steam for 20 min. You can adjust the pH, but you don't need to adjust it, and it's around the right and right.

Medium is an artificially formulated nutrient for the growth and maintenance of microorganisms, plant tissues and animal tissues, generally containing carbohydrates, nitrogenous substances, inorganic salts (including trace elements), vitamins and water. Different media can add some compounds that cannot be synthesized by themselves, that is, growth factors, according to actual needs. Some microorganisms, such as autotrophic microorganisms, do not require a carbon source, so the substances mentioned above are only general.