Brineco prepares plasmids in large quantities spin column type .

Prolinco Answers: Plasmid Mass Preparation Kit (Spin Column Type) for Large-Scale Plasmid Preparation Item No. Packaging DP2501 5 times DP2502 10 times Product Description: This kit uses the improved SDS-alkali lysis method to lyse cells, the silica matrix membrane in the centrifugal adsorption column selectively binds the plasmid DNA in the solution under the state of high salt and low pH value, and then removes impurities and other bacterial components through protein removal solution and rinse solution, and finally low salt, The high pH elution buffer elutes the pure plasmid DNA from the silicon-based plasma membrane.

Kit features: The silicon matrix membranes in the centrifugal adsorption column are all specially made by imported world-famous companies, and the difference in adsorption capacity between columns is very small, and the reproducibility is good. It overcomes the disadvantages of unstable membrane quality of domestic kits.

There is no need to use toxic phenol, chloroform and other reagents, and there is no need for ethanol precipitation. Fast and convenient, from 100-200 ml of Escherichia coli LB (Luria-bertani) culture, Mg of pure, high-copy plasmid DNA can be quickly extracted with an extraction rate of 85-90. The obtained plasmid has high yield, high supercoiling ratio and good purity, and can be directly used in various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, and sequencing.

Beijing Deyuan International Technology Co., Ltd. can carry out quality extraction and can be contacted.

Endotoxin-free high-purity plasmid small amount rapid extraction kit (spin column type): special for sequencing, SunBiotech plasmid kit, special for sequencing with high yield and excellent purity, tracking statistics of 220,000 sequencing samples, sequencing success rate is 12% higher than that of domestic kit, and the length of 100-300bp imported membrane is the best choice for your sequencing. Features:

Sequencing only! Bacterial endotoxin is unique to the cell wall of gram-negative bacteria, and its main component is lipopolysaccharide, and the endotoxin contaminated in the lipid solution is an important reason for the low transfection efficiency of primary cultured cells. This kit uses an improved SDS alkali lysis method to lyse cells, the crude extract is selectively combined and centrifuged by a unique endotoxin scavenger to remove endotoxin, then the silica gel membrane in the centrifugation adsorption column selectively binds the plasmid DNA in the solution under high salt and low pH state, and then the impurities and other bacterial components are removed by deproteinization solution and rinse solution, and finally the pure plasmid DNA is eluted from the silica membrane with low salt and high pH elution buffer. Purified plasmid endotoxin < eu ug.

Kit Features:

The imported silica gel membrane is adopted, which has large adsorption capacity, high yield and good reproducibility of experimental results.

The unique deproteinization solution formula can efficiently remove residual nucleases, even strains with rich nuclease content such as JM series and HBL01. Effectively prevents the plasmid from being degraded by residual nucleases.

A unique endotoxin removal formula that is generally less effective than < Eu ug.

It is fast and convenient, and does not require the use of toxic reagents such as phenol and chloroform, nor does it require ethanol precipitation. The obtained plasmid has high yield and good purity, and can be directly used for various molecular biology experiments such as transfection, enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.

Prolinco's High Purity Plasmid Extraction Kit can quickly maximize the availability of high-purity plasmids from a large number of bacterial fluids.

This kit uses a unique buffer system and the addition of Tiangen's special color indicator to ensure that a large number of high-purity plasmids can be obtained more efficiently. The use of this kit can flexibly select the initial volume of the treated bacterial solution according to different experimental needs, and use the traditional isopropanol precipitation method to quickly obtain a large number of high-purity plasmid DNA to meet the needs of a variety of follow-up experiments.

Features: High yield: direct precipitation of isopropyl hail alcohol, the highest quality source of nightshade DNA can be obtained.

Good purity: Processed with buffer P4 and filter CS to meet the requirements of most transfection experiments.

Easy and fast: just a few simple centrifugation steps, and the experiment can be completed in about 1 hour.

Widely used: suitable for molecular biology experiments such as enzyme digestion, PCR, transformation, sequencing and transfection, color indicators make your plasmid extraction more efficient.

Storage conditions: Room temperature.

Brineco offers a miniprep kit for high-purity plasmids.

Principle introduction: This kit uses an improved SDS-alkali lysis method to lyse cells, the silicon matrix membrane in the centrifugal adsorption column selectively binds the plasmid DNA in the solution under the state of high salt and low pH value, and then removes impurities and other bacterial components through deproteinization solution and rinse solution, and finally the pure plasmid DNA is eluted from the silicon matrix membrane with low salt and high pH elution buffer.

Precautions: For the first use, add all RNAse A from the kit to solution P1 (final concentration 100ug ml) and store at 4 μg. If RNase A is inactivated in solution P1, the extracted plasmid may be mixed with trace amounts of RNA residue, so RNase A can be added to solution P1.

Please add 45ml of absolute ethanol to 15ml of rinse solution WB before using it for the first time, and mark the added ethanol in the box in time after adding it, so as not to add it multiple times!

When the temperature is low, the SDS in the solution P2 may appear turbid or precipitate, which can be heated in a 37 water bath for a few minutes to restore clarification, do not shake vigorously to avoid the formation of excessive foam.

To avoid volatilization, oxidation, and pH changes caused by long-term exposure of the reagent to the air, the lid should be closed tightly in time after the use of each solution.

Kit Features:

High yield--- up to 30ug of plasmid can be extracted at a time.

High purity--- OD260 OD280 is generally the plasmid extracted by this kit, which has good purity and can fully ensure the read length required for sequencing (for ABI3730 sequencing, it can generally reach 1000bp effective read length).

It is fast, convenient, does not require the use of toxic phenol, chloroform and other reagents, and does not require ethanol precipitation.

This suggests that Bioteke's plasmid extraction kit is suitable for the extraction of plasmids from both gram-negative bacteria and plasmids from gram-positive bacteria. Since Gram-positive bacteria are covered by a thick cell wall, which can severely hinder the lysis of bacterial cells, they must be broken before lysing the cells in the following ways:

Collect an appropriate amount of bacteria, add 250ul solution P2, fully suspend the bacterial solution, add lysozyme to make its final concentration at about 10-20mg ml, and treat it at 37 for about 30 minutes. The concentration of lysozyme and the treatment time can be adjusted according to different bacterial owners and specific experimental conditions.

The unique extraction and lysis system of Bolingke for fecal genomic DNA rapid extraction kit (spin column type) quickly lyses cells (walls) and inactivates intracellular nucleases, without the help of glass beads to break the wall, effectively ensuring the integrity of genomic DNA, and the specially treated purification column can effectively remove impurities and humic acid, greatly improving the purity of DNA, and the extraction of genomic DNA with this kit is of high purity and is suitable for downstream reactions such as PCR.

Note: All centrifugation steps can be performed at room temperature using a conventional benchtop centrifuge such as the Eppendorf 5415C or similar that can reach speeds up to 13,000 rpm.

Solution B is easy to form a precipitate at low temperature, which can be dissolved in a 65 water bath.

Kit Features:

The specially treated cartridge effectively removes impurities and humic acids.

It is highly compatible and suitable for a variety of different feces.

There is no need to use reagents such as toxic phenol, nor do steps such as ethanol precipitation occur.

Fast and simple, a single sample can be completed in less than 60 minutes.

High purity, OD260 OD280 typical ratio, length up to more than 50kb, can be directly used in PCR, Southern-blot and various enzyme digestion reactions.