How breast cancer cells are cultured, breast cancer cell treatment

Bolinco for biotechnology - answer for you: the cells raised are all breast cancer cells, the following types: 1. MCF-7 is a very good human breast cancer cell, the medium can be used DMEM or RPMI1640, 10 15 calf serum can grow very well.

Generally, it is passed on to one generation in two to three days. Passage is also routine. Aspirate the medium, add 1ml of trypsin, and gently shake the flask to make the trypsin flow all over the wall of the bottle to remove the residual medium.

Then add 2ml of trypsin (25ml culture flask) and let it stand for digestion for 2 5min, wait for the cells to shrink and become round, aspirate the trypsin and add 3ml of culture medium to blow into a single cell suspension flask. I usually don't use buffer to rinse, but directly rinse with pancreatic enzymes to remove the effect of the remaining serum, and I feel that the effect is not bad. Because MCF-7 is digested quickly, it is generally not put into the incubator for digestion to avoid over-digestion.

It should be noted that MCF-7 grows rapidly, and if it is not passaged in time, the whole bottle of cells may float, and it is generally too late to rescue. 2. T47D is also a kind of human breast cancer cells, the medium is DMEM 10 15 calf serum, the culture method is similar to MCF-7, but this kind of cell passage time will be extremely difficult to raise. I once raised a plant that was newly purchased from the United States, and it was passed on to one generation in two or three days, and it grew very vigorously.

Later, I bought a longer plant from China, which took seven days to pass on to one generation, and it was very squeamish. 3. MA891 murine breast cancer cells, this kind of cell is special and difficult to digest, easy to digest into a clump, if the amount of pancreatic enzyme is more than one-third of the commonly used, fully preheated and put into the incubator to digest it will be much better. When it reaches 15 to 20 generations, the cells are easy to grow into clumps, and from then on, they grow slowly, change their morphology, and need to be revived.

How do you get breast cancer? i.e. the causes of breast cancer:

First, it is related to the high level of hormones in the body, especially the most common estrogen and progesterone secretion, which is also one of the most important causes of breast cancer.

Second, it is related to the emotional changes of the body, especially the love of tantrums and sulking.

Third, it may be related to underwear that is too tight, ill-fitting, etc.

The development of breast cancer requires a comprehensive **.

Cells are tumor organisms, which are infused tens of millions of times by cultivating human autoimmune cells and killer factors in an attempt to achieve the purpose of anti-cancer. You can ask patients or family members who have used this method, it will be effective in preventing metastasis within a certain period of time, but thousands of troops cannot catch thieves who have not committed crimes, and it is ineffective to deal with cancer cells in the tumor, and its role is very limited without initiative, and there is nothing that can be done about prevention and control during the non-action period. Improving the body's immunity is the foundation of fighting cancer, and the key is to play the role of recognition; As long as it can be discovered, it can be annihilated in one fell swoop.

Immunity is so powerful, and it doesn't need to be cultivated by people to have such a function, it depends on how you choose to apply it. The cases of orange difference that naturally resolves tell people that their **most will not**, this is the principle of hope; The developed technology is carried out around human immunity, providing pathways, pathways, and places for immune cells to fight cancer cells, immune cells can grow and replenish as needed, and cancer cells are inhibited and gradually die. When you are older, the method of not harming the human body is worth paying attention to, and the round suspicion skin reference is.

Last week, we went to see a friend's mother, liver cancer, and now we are doing hail cell excavation**, in Beijing Wanbo Traditional Chinese Medicine Hospital, I heard my buddy say that the effect is quite good, and now they have a cell biodonation project.

Most of them use the MCF-7 cell line, which is the subject of many drug screening tests, and it grows more slowly, so be patient.

It is more difficult to operate by yourself. Generally, a surgeon with experience in waxing socks will play this anesthetic middle acre, and it is ineffective if the rotation training is not good.

It is better to go to a professional medical institution hospital or clinic, which will be more professional. The effect is also relatively good.

It's better to go to the lilac garden and ask questions. What kind of medium do you use when buying cell lines, the company has instructions!

Breast cancer cells have metastasized to other parts of the body, and now what are the symptoms of the patient, you can talk about it in detail, the most effective for metastatic and spread cancer is to use traditional Chinese medicine to distinguish the disease, which can effectively control the disease, and effectively prolong the survival time of the patient.