Introduction to the gel imaging system, does the gel imaging chromatograph have an analysis function

Gel imaging is the detection and analysis of different stains of DNA RNA and protein by gel electrophoresis (such as EB, Coomassie brilliant blue, silver staining, SYBR Green) and non-chemiluminescence imaging of microplates and plates. The gel imaging system can be applied to molecular weight calculation, density scanning, density quantification, PCR quantification and other routine bioengineering research.

Gel imaging is very simple, it is equivalent to a camera, an ultraviolet transilluminator stage, a darkroom, for photography. Just read the manual how to take pictures on the computer, the camera will make it, right? Adjust the focal length, focus, aperture of the aperture, if you don't know how to use a camera, please move to make up for the basic knowledge of photography.

Gel imaging system is a commonly used instrument in laboratories, and with the gradual popularization of molecular biology research, the demand for gel imaging system in China is growing. Gel imaging systems can be used in a wide range of applications. Overall, gel imaging systems can be used for:

Qualitative analysis of the separation and purification results of other biomolecules such as proteins, nucleic acids, peptides, amivotroic acids, polyamino acids, etc.; determine the molecular weight of a biomolecule; It is used for the quantitative analysis of biomolecules. Specifically, from the perspective of use analysis, the gel imaging system can be applied to routine bioengineering research such as molecular weight metering, lead hall calculation, density scanning, density determination, PCR quantification, etc.

The mobility of the sample on the electrophoresis gel or other support is not the same, and the unknown sample will be qualitatively analyzed by comparing it with a standard or other surrogate standard. This is the qualitative basis of image analysis systems. Qualitative analysis of an unknown sample can be performed based on its location in the spectrum, and its composition and properties can be determined.

The sample partially absorbs the projected or reflected light, so that the optical density of the sample bands on the photographic image will vary. The optical density is linearly related to the concentration or mass of the sample. According to the optical density of an unknown sample, the concentration or mass of an unknown sample can be obtained by comparing the optical density index of the sample bands of known concentration.

This is the basis of quantification in image analysis systems. The use of the latest ultraviolet transmission light source and white light transmission light source makes the light distribution more uniform, and the influence of uneven optical density on the result is eliminated to the greatest extent.

The gel imaging analysis system can observe and analyze a variety of transparent or opaque electrophoresis images, such as EB staining gel, protein gel, autoradiography, blotting, spot blotting, etc., to meet the urgent needs of qualitative and quantitative analysis. It provides an advanced and easy-to-use solution for gel image analysis. It provides a quick solution for the majority of researchers engaged in molecular biology, hospital clinical examination, and forensic physical evidence.

It provides an easier, faster, and more accurate way to reproduce, collect, and analyze details in images. It is composed of image shooting, image processing, data analysis, report printing, etc., which is in line with the operation ideas of conventional experiments, and is convenient, practical and simple to help researchers get the gel electrophoresis results correctly and quickly.

1 Turn on the gel imaging system switch.

2 Turn on the computer, open and enter the imaging software.

ECL shooting.

Switch the shooting mode to "ECL mode" and turn the filter wheel to the ECL bit. Select the appropriate shooting resolution (this is available on machines with binning) and click "Start". Place the sample in the middle of the sample stage, adjust the focal length of the lens so that the sample occupies about 80% of the window, then click "Auto**", check off the "negative" and adjust the focus to make the sample image in the preview window clear.

The wider the aperture, the shorter the time required for auto**. And shoot with a single frame first, taking a mark**. After turning off the reflected white light, the nitrocellulose membrane placed on the chemiluminescence imaging plate is evenly added with the luminescent solution.

Set the shooting method to "Rule Credit", tick "Negative", set the time and number of shots, and click the "Shoot" button.

Ordinary gel shooting.

1 Switch the shooting mode to "Normal mode" and turn the filter wheel to the UV position (no adjustment is required for those without green lens wheels).

2 Select the appropriate shooting resolution (the machine has a pixel binning function) and click "Start".

3.DNA gel shooting: Place the DNA gel in the middle of the UV stage, adjust the focus so that the sample occupies about 80% of the window, then click "Auto**", and adjust the focus to make the sample image in the preview window clear.

Then turn off the reflected white light, turn on the transmitted UV, and fine-tune to ensure that it is in a clear state under the ultraviolet light.

Protein gel shooting: Place the protein gel in the middle of the disassembled white light plate, turn off the reflected white light, turn on the transmitted white light, and then click "Auto**", and adjust the focus to make the sample image in the preview window clear.

4.Click the "Shoot" button on the software interface.

1. Is it true that the higher the pixels, the better the product?

Pixels are for imaging equipment, for example, the pixels of digital cameras and CCDs are not comparable, and secondly, the quality of CCD itself is more important than the simple pixel level. The most important indicator for the same level of CCD is its size, that is, the size of the CCD, the larger the size, the value of itself increases exponentially.

2. What is the main purpose of the UV reflector lamp source of the accessories?

Ultraviolet reflector lamp sources are not widely used, mainly to provide non-transparent DNA gel carriers, such as paper chromatography, etc., for imaging needs and sensitive use. Since the more commonly used branches are transparent agarose gels and polyacrylamide gels, the transmission light source can be fully satisfied, so the ultraviolet reflection light source is not included in the standard configuration as an option.

3. Can I cut the gel directly on the gel imaging?

If you need to cut the gel directly, first open the observation window equipped with imported anti-ultraviolet glass according to the observation window in the middle of the system, and then open the small doors specially designed for rubber tapping on the left and right sides, and then you can cut the gel.

4. How to adjust the focal length and focus aperture to get high quality**?

The three most important adjustable parameters are focal length, focus, and aperture. Among them, the focal length adjusts the sharpness, the focus adjusts the image size, and the aperture adjusts the brightness and darkness. Generally, after adjusting the aperture to the appropriate brightness, then adjusting the focus to the maximum image, and finally adjusting the defocal length, the focal length is easier to adjust to the clearest state in the case of a large image, and then the image can be scaled to a suitable size.

Gel imaging is the detection and analysis of different stains of DNA RNA and protein by gel electrophoresis (such as EB, Coomassie brilliant blue, silver staining, SYBR Green) and non-chemiluminescence imaging of microplates and plates. The gel imaging system can be used in the routine study of bioengineering such as molecular careful search calculation, density scanning, density quantification, and PCR quantification.