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Before using freeze-dried seedlings (live seedlings), ducks should be inoculated only after dilution.
1) The dilution solution is generally normal saline, but distilled water can also be used.
or cool boiled water. For vaccines that require special dilution, the specified dilution should be applied. If the vaccine itself is equipped with a diluent, the diluent is used.
Do not dilute the vaccine directly from tap water, well water or river water. The dilution should be cool, especially in hot weather. The amount of diluent should be calculated and weighed carefully and accurately.
2) The diluted dose should be strictly in accordance with the dose specified in the vaccine instructions, and should not be arbitrarily increased or decreased, otherwise it may lead to immunization failure. For example, duck plague chicken embryo attenuated vaccine, according to its prescribed dose, dilute it 200 times with 200 ml of normal saline, and inject it intramuscularly into each duck.
1 ml. 3) Dilute utensils such as syringes.
Needles, tweezers.
etc., should be strictly disinfected before use.
4) The dilution process should be protected from light, wind and dust and aseptic operation.
In particular, vaccines for injection should be strictly aseptic. The dilution process should generally be carried out in stages, and the vaccine bottle should be rinsed with diluent 2 to 3 times.
5) A sterilized needle should be fixed on the diluted vaccine bottle and covered with alcohol cotton.
6) The diluted vaccine should be used up as soon as possible, and the unused vaccine should also be refrigerated in the refrigerator or ice bucket.
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When dilution, it should be carried out according to the specified amount of head and diluent in each bottle.
2. If the vaccine has a special diluent, it must be diluted with a special diluent.
3. Regardless of distilled water, normal saline, buffered saline, aluminum glue saline, etc., it is required to be free of foreign impurities and immutable. In particular, it is required that the various dilutions should not contain any pathogenic microorganisms, nor can they contain any disinfectants. If you make distilled water, normal saline, buffered saline, etc., you must be disinfected and cooled before use.
4. Dilution utensils such as syringes, needles, droppers, dilution bottles, etc., are required to be cleaned in advance and autoclaved for later use.
5. When diluting the vaccine, it should be diluted several times according to the number of chickens and the number of people participating in immunization, and the diluted vaccine should be used up within 1 hour at room temperature. MD liquid nitrogen seedlings CVI988 are required to be used up within half an hour.
6. The vaccine or dilution that has been uncorked must be used up at the same time. If it is not used up, it should not be retained, it should be discarded and treated harmlessly.
7. Vaccines and diluted vaccines cannot be packed in metal containers, and buffered saline and aluminum glue saline should be used after full shake when using buffered saline and aluminum glue saline as diluent. 8. When diluting liquid nitrogen seedlings (such as Maric CVI988), special attention should be paid to the correct operation (for detailed operation, please refer to the instructions for the use of liquid nitrogen seedlings prepared by various manufacturers).
9. When carrying out drinking water immunodilution vaccine, attention should be paid to water quality, preferably with deep well water, and add skimmed milk powder first, and then add vaccine. Care should be taken not to use chlorinated or bleach-treated tap water so as not to affect the quality of immunity.
In order to make the chicken vaccine more effective, I hope that the major farmers can strictly follow the above precautions when diluting the chicken vaccine.
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In the production process of raising chickens, most people know that as long as they do a good job in the prevention of chicken plague (Newcastle disease), even if they are half of the success of raising chickens, they also know that chicken plague is prevented by chicken plague vaccine (Newcastle disease vaccine), and these understandings cannot be said to be wrong. But the problem is not so simple, because the vaccine to prevent chicken plague is not one, and its usage is not the same, chickens of different ages use different vaccines, and if they are not well mastered or used inaccurately, the epidemic prevention work will fail. To this end, the knowledge of chicken plague vaccine and its use are introduced as follows, for the reference of farmers and herdsmen and professional households who raise chickens.
There are several types of vaccines to prevent chicken plague:
The chicken plague II attenuated vaccine is used by nasal drop at the age of 7 to 9 days of age.
Chicken plague "III" and "IV" vaccines are used in the chick at 10 days of age, the vaccine is diluted into a concentration with normal saline, so that the chicks can drink freely, each chick should drink 10 ml to 15 ml, the immunization period is more than 7 months, do not dilute with hot water, so as not to reduce the effect.
The vaccine is infused into the body to improve immunity and fight some harmful viruses. As long as the immunity is strong, the body will fight off a lot of viruses.
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