How to use each part of the microscope, how to use the microscope

What kind of microscope exactly do you mean by microscope?

1. Adjust the brightness: from dark to bright, you can use a large aperture and a concave mirror to adjust the angle of the reflector.

2. Fix the temporary loading at the appropriate position on the stage.

3. The low-magnification objective lens is aligned with the clear hole, and the lens barrel is adjusted from top to bottom using a coarse collimation spiral, so that the eyes can observe from the side to avoid the objective lens from touching the slide and damaging the lens and crushing the slide.

4. The left eye observes the changes in the visual field through the eyepiece, and at the same time adjusts the coarse quasifocal spiral to slowly move the lens barrel up until the visual field is clear.

5. If there is no object to be observed in the field of vision, you can move the film, the principle is to want to go up and down, and want to go left and right.

Care of the microscope.

1) After using the oil lens, first wipe off the oil on the lens with lens polishing paper, then take a piece of lens polishing paper, drip a small amount of xylene to wipe it, and then take another new lens polishing paper to wipe off the residual xylene on the lens. Otherwise, the gum of the adhesive lens will be dissolved by xylene, and the lens will easily shift and fall off over time.

2) Lower the condenser and open the iridescent aperture so that the mirror is perpendicular to the mirror base to avoid dust accumulation.

3) Wipe the mirror body with a silk cloth (do not wipe it by hand) to remove dust, oil and water vapor to avoid rust and mildew.

Alignment: Turn the converter so that the low-magnification objective is facing the clear aperture and the front of the objective is about two centimeters away from the stage. Then point the mirror at the light source, but the mirror cannot be directly pointed at the sun. As long as the brightness of the field of vision is appropriate, the light is right.

Observation: After setting the light, place the microscope slide specimen on the stage, and make the specimen on the slide face the center of the light hole, and then clamp it with a tablet clamp. Then slowly turn the focus spiral until you are close to the slide.

Next, gaze into the eyepiece with your left eye while turning the focus spiral in the opposite direction until you see the object clearly.

Magnification: Select a magnification target, move the part to be magnified to the center of the field of view, and if the object image is not clear, then turn the focusing spiral. If the observation is still unclear, it is necessary to switch to a high-magnification objective.

When observing with a high-power objective, the tip of the high-power objective is very close to the slide, and if you are not careful, the lens will press against the slide, so be especially careful.

The image seen in the microscope is an inverted image, so to move the image upwards, you need to move the slide downwards, and to move the image to the left, you need to move the slide to the right.

There are four steps to using a microscope. The first step is to take the lens and place it, the second step is to light it, the third step is to observe, and the fourth step is to organize.

Lens collection and placement :1Hold the temple arm with your right hand and hold the lens base with your left hand. 2.Place the microscope on the bench, slightly to the left (the microscope is about 7 cm away from the edge of the bench). Attach the eyepiece and objective.

To the light: 1Turn the converter so that the low-magnification objective is aligned with the clear aperture (keep the front end of the objective 2 cm away from the stage).

2.Aim a larger aperture at the clear aperture. The left eye is fixed inside the eyepiece (the right eye is open so that the picture can be drawn at the same time later).

Turn the mirror so that the light is reflected into the barrel through the aperture. Through the eyepiece, you can see a white and bright field of vision.

Observations:1Place the slide specimen (which can also be made from a thin piece of paper with the word 6 printed on it) on the stage and press it with a tablet clamp facing the center of the aperture.

2.Turn the coarse collimation spiral so that the barrel is slowly lowered until the objective lens is close to the slide specimen (eyes look at the objective so that the objective does not touch the slide specimen). 3.

The left eye looks into the eyepiece and turns the coarse procal spiral in the opposite direction so that the barrel slowly rises until the object is clearly visible. Then turn the fine collimation spiral slightly to make the image of the object seen more clearly.

Finishing: After the experiment, wipe the surface of the microscope clean. Turn the converter to offset the two objectives to the sides and slowly lower the barrel to the lowest point with the mirror in an upright position. Finally, put the microscope in the case and return it to its original place.

The use and operation of the microscope is simpler than the production of slide specimens, and after understanding the structure of each part of the microscope, the most important thing is to grasp the order of use, and the rest will be easy.

1. Take the mirror and place it.

Take the lens with both hands: hold the mirror arm with one hand and hold the lens holder with the other hand, and place it 7 cm away from the edge of the laboratory table or a fist. (It is forbidden to take the lens with one hand).

Second, to the light. Once you've put it in place, it's time to turn on the light. When aligning the light, first raise the lens barrel, then turn the converter so that the low magnification objective lens is aligned with the clear hole, one eye is fixed on the eyepiece, the other eye is open, and the reflector is rotated to brighten the field of vision.

In the process of aligning the light, it is necessary to rotate the converter, and do not use a hand to trigger the nosepiece, because when the low-magnification objective lens is facing the light hole, the sound can be heard. When turning the reflector, you can choose a flat or concave mirror according to the intensity of the light, and after the light is successfully aligned, you can see a white field of vision in the eyepiece. The position of the microscope can no longer be moved.

3. Place the slides.

After aligning the light, place the slide specimen face up on the stage, facing the center of the light aperture, and press the ends of the slide with a clamp.

Fourth, focusing. When focusing, use your eyes to look at the objective lens from the side to descend to the lowest, so that the objective lens is close to the slide specimen and cannot crush the coverslip, one eye is fixed on the eyepiece, turn the coarse collimation spiral, so that the lens barrel rises slightly, you can see the object image in the field of view, and fine-tune the fine collimation spiral to make the object image clearer.

5. Observation. One eye looks into the eyepiece and the other eye is open for observation. Under the microscope, what is observed is a magnified inverted virtual image, and if the object image is biased to the upper left in the field of view, the specimen should be moved to the upper left in order to appear in the field of view.

After the experiment is completed, the lens barrel is raised, the glass specimen is removed, the microscope is wiped clean, the converter is rotated, the objective lens is deviated from the light aperture, and the lens barrel is lowered to the lowest point, the mirror is placed vertically, put it into the lens box, and send it back to its original place.

Remember to clean up, wipe down and put the specimen back in its place.

1. Take and place When using, first take out the microscope from the lens case, you must hold the lens arm with one hand, hold the lens holder with the other, keep the lens body upright, and never use one hand to tilt and carry it to prevent the eyepiece from falling. Handle with care, with the temples facing you, 5-10 cm from the edge of the table. The table is required to be balanced, the top is clean, and it is protected from direct sunlight.

2. Turn on the light source and turn on the power switch.

3. Place the slide specimen The slide specimen to be examined is placed on the stage so that the material is facing the light hole**. The slide is then clamped at both ends with a spring pressure to prevent the slide specimen from moving. In the case of a slide movers, snap the slide specimen into the slide mover, then adjust the slide mover to move the material directly opposite the clear hole**.

4. Low-magnification objective observation When observing specimens with a microscope, the object image should be found with a low-magnification objective lens first. Because of the large observation range of the low-magnification objective, it is easier to find the object image and find the parts that need to be carefully observed. The method is as follows:

1) Turn the coarse adjustment spiral and look from the side with your eyes to lower the lens barrel until the low magnification objective lens is about a centimeter away from the specimen.

2) Observe from the eyepiece with your left eye, open your right eye naturally, and slowly turn the coarse adjustment spiral with your hand so that the lens barrel gradually rises until the image in the field of vision is clear. After that, the fine-tuning spiral was used, and the focal length was slightly adjusted to make the image the clearest.

3) Gently move the slide forward, backward, left and right with your hand or adjust the slide mover to find the part you want to observe. It should be noted that the object in the field of view is inverted, and the slide should be moved in the opposite direction.

The above content is the detailed steps of the use of the microscope, and warmly reminds us that we must follow the operating procedures when using the microscope, be careful and patient, and do not rush or act too violently to prevent damage to the components due to operation errors. Do not touch the optical glass with your hands to prevent damage to the components due to violent collisions.

If you want to use the microscope, you need to hold the wall of the mirror with one hand, lift the lens holder with the other hand and take out the microscope, adjust the mirror with your eyes facing the eyepiece, lift the lens barrel and put the slide specimen into the tablet clamp, then lower the lens barrel, look at the eyepiece with your eyes, and adjust it with a coarse collimation spiral until you can see the cells.

Hold the wall of the microscope with one hand and the holder of the microscope with the other hand so that the microscope can be safely removed.

Align your eyes with the eyepiece and place your hand near the mirror until the field of view appears aperture, and continue to adjust the focus of the microscope.

Raise the tube so that the objective lens is separated from the stage so that it is convenient to wait for the specimen to be observed.

Open the clamp, put the slide specimen on it, and place the slide specimen in the center of the light hole.

Turn the coarse collimation spiral and slowly lower the lens barrel until the objective lens stops when it approaches the slide specimen, at which point your eyes are aligned with the eyepiece and adjust the coarse collimation spiral as you observe until you see the cells in the microscope.