What are the rules of aseptic operation of medical devices and the principles of aseptic operation?

Principles of aseptic operation:

1. The environment should be clean, and half an hour before aseptic operation, the cleaning of the ground and other work must be stopped. Avoid unnecessary crowd movements and prevent dust from flying. **The room should be disinfected with ultraviolet light once a day.

2. When performing aseptic operation, the sterile and non-sterile areas of the article must be clarified.

3. Before performing aseptic operation, wear a hat and mask, wash your hands, dry your hands, and pay attention to air and environmental cleanliness.

4. To clamp sterile items, sterile forceps must be used. When performing aseptic operations, unsterilized hands and arms should not directly touch sterile items or take materials beyond the sterile area.

5. Sterile items must be kept in a sterile bag or sterilization container, and should not be exposed to air for too long. Sterile and non-sterile materials should be placed separately. Once opened, a sterile bag cannot be considered absolutely sterile and should be used as soon as possible.

Sterile items that have been removed cannot be put back into the sterile container even if they have not been used.

6. The sterile bag should be placed in the fixed cabinet in the order of the disinfection date, and kept clean and dry, separated from the non-sterilized bag, and frequently check whether the sterile bag or container is expired and whether the materials used in it are appropriate.

7. Sterile saline and alcohol, Xinjieer cotton ball tank should be disinfected once a week, and the dressings in the container, such as dry cotton balls, gauze blocks, etc., should not be overfilled, so as not to be contaminated outside the container when taking it.

When cultivating, you need to be precise and agile, but not too fast, to prevent air movement and increase the chance of contamination. Do not touch the sterilized utensils with your hands, and if they do, they should be cauterized with a flame or replaced with spare parts.

For ease of access, the supplies on the work surface should have a reasonable layout, in principle, the things used by the right hand should be placed on the right side, the left hand supplies on the left side, and the alcohol lamp should be placed on the right side. The work should be done in a certain order from beginning to end, and tissues or cells should not be exposed to air too early before they have been treated.

Similarly, the culture medium should not be opened too early before it is used; If you do not reuse it after use, you should close the bottle immediately and stand upright to increase the chance of colonization. When aspirating nutrient solution, PBS, cell suspension and other solutions, pipettes should be used separately and should not be mixed to prevent expanded contamination or cross-contamination of cells.

There are 11 articles in total, and I went to see the 11 pages of the sixth edition of the People's Medical Publishing House's Department of Surgery!

The operating principles of aseptic technique are as follows:

1. When carrying out aseptic operation, it is necessary to clean the environment, disinfect regularly, arrange the items reasonably, remove bacteria and microorganisms, stop cleaning 30 minutes before operation, and the operation area must be wide to facilitate the operation, close the door in time to avoid the entry of bacteria and microorganisms, and be rigorous in the process of frequent personnel action.

2. Operators must pay attention to their attire, wear sterile gowns, wear masks and hats, trim their nails and wash their hands before operation, and wear sterile gowns and sterile gloves before operation.

3. When carrying out aseptic operation, the concepts of sterile area, non-sterile area, and sterile bend-resistant items should be clarified first.

4. Sterile items must be placed separately from non-sterile items, and must be clearly marked; Sterile items should not be exposed to air and should be placed in a sterile bag or sterile container; The name of the item and the date of sterilization should be marked on the outside of the sterile package, and placed in the order of expiration date; The shelf life of the sterile package is generally 7 days, and it should be re-sterilized after expiration or moisture.

5. When performing aseptic operation, the operator's body should be kept at a certain distance from the sterile area; When picking up and placing sterile items, they should face the sterile area; Sterile forceps should be used when using sterile items; Sterile items should not be touched by the arm; Once the sterile item is taken out, it cannot be put back into the sterile container; Avoid coughing and sneezing in the sterile area; If the material is suspected to be contaminated or has been contaminated, it should be replaced and re-sterilized; Non-sterile items should be kept away from the sterile area.

6. A set of sterile items can only be used once by one patient. to prevent cross-infection.

The operating principles of aseptic technology are as follows: environmental cleaning, aseptic operation, article management, sterile goods, sterile material retrieval, and aseptic operation.

1. Clean environment

Half an hour before the aseptic technical operation of the beam width, it is necessary to stop cleaning the floor and other work to avoid unnecessary crowd flow and reduce the movement of personnel to reduce the dust in the indoor air. Prevent dust from flying. **The room is disinfected with ultraviolet irradiation once a day for 20-30 minutes, and the disinfection time can also be appropriately extended.

2. Aseptic operation

Wear a hat neatly and neatly. Hats should cover all hair, masks should cover nose and mouth, nails should be trimmed, and hands should be washed. Wear sterile gowns and gloves if necessary.

3. Item management

Sterile articles and non-sterile articles should be placed separately, sterile articles should not be exposed to air, must be stored in a sterile bag or sterile container, once the sterile goods are used, they must be aseptically treated before they can be used, and the items taken out of the sterile container, although not used, can not be put back into the sterile container.

4. Sterile items

Sterile items must be stored in a sterile bag or sterile container, the sterile bag should be marked with the name of the sterile, the date of disinfection and sterilization, and the validity period of rubber brightening is one week, and the order of the date is discharged, so that it can be taken and placed in a fixed place. Sterile packs can be stored for 7 days without contamination and should be re-sterilized after expiration. Once the sterile goods have been used or expired, they should be re-sterilized when they are wet.

5. Take sterile material

The operator is 20cm away from the sterile area, and the sterile holding forceps (forceps) must be used when taking the sterile goods, and the sterile materials should not be touched or crossed across the sterile area, and the arms should be kept above the waist. After the sterile item is removed, it should not be exposed for too long, and if it is not used, it should not be put back into the sterile bag or sterile container. If it is suspected of pollution, it shall not be used.

Unsterilized items must not touch the sterile material or cross the sterile area.

6. Aseptic operation

If the instrument or substance is suspected to be contaminated or has been contaminated, it cannot be used and should be replaced or re-sterilized. One item per person, one set of sterile items, can only be used by one patient to avoid cross-infection.

The following principles should be followed for performing aseptic technical procedures:

1. The environment is clean and spacious: the environment should be disinfected regularly, and the work of sweeping and changing bed sheets should be stopped half an hour before operation, so as to reduce walking, avoid unnecessary crowd flow, and prevent dust from flying.

2. Before aseptic operation, staff should be neat and tidy, wash hands and wear masks.

3. Orderly management of items:

1) Sterile items must be placed separately from non-sterile items and clearly marked.

2) Sterile items should not be exposed to air and should be stored in sterile bags or sterile containers.

3) The name of the item and the sterilization date should be marked on the outside of the sterile package, and they should be placed in the order of expiration date.

4) Sterile forceps should be used when taking sterile items. (5) Once the sterile items are taken out, although they are not used, they shall not be put back into the sterile Fangzhou container.

6) If the article is suspected or has been contaminated, it cannot be used and should be replaced and re-sterilized.

4. Clarify the sterile area and non-sterile area: the operator's body should keep a certain distance from the sterile area; When picking up and placing sterile items, they should face the sterile area; The arm should be kept above the waist or ** table, not across the sterile area, and the hand should not touch the sterile material.

Avoid laughing, coughing, and sneezing in front of the sterile area; Unsterilized items are not allowed to touch sterile hall items or cross dust-free areas.

5. A set of sterile items is only for one patient to prevent cross-infection.

Principles of aseptic technique: a way to control pathogenic microorganisms and avoid infection in nursing.

The operating principles of aseptic technique are as follows:

1. First of all, aseptic operation should be carried out in a sterile environment.

2. The operator must bring a mask, a hat, wear a sterile gown, and then wear a good sterile glove, at this time you can contact the sterile goods, and then make the hall with sterile items for aseptic operation, in the aseptic operation must not touch the bacterial substance, once touched, immediately stop the aseptic operation to avoid bacterial infection. Therefore, the basic operating principle of aseptic technology is to strictly carry out aseptic operation, and no fungal matter can be touched during the operation.

1.Preparation before operation.

1) The operating environment should be clean, spacious and regularly disinfected; The layout of the items is reasonable; Half an hour before aseptic operation, the cleaning work should be stopped, walking should be reduced, and dust should be avoided.

2) Staff should be personally prepared, wear hats, masks, trim nails and wash hands, and wear sterile gowns and gloves if necessary.

2.Maintain sterility during the procedure.

1) The working posture should face the sterile area, and the arms should be kept above the waist or the countertop, and should not cross the sterile area to avoid talking, laughing, coughing, and sneezing in the face of the sterile area.

2) Pick up the item with sterile holding forceps; Once sterile items are removed, they should not be put back into the sterile container, even if they are not used; A set of sterile rubbers is for one patient only to avoid cross-infection.

3) During aseptic operation, sterile items suspected of being contaminated or have been contaminated should be replaced and re-sterilized.

3.Sterile storage.

1) Sterile items must be placed separately from non-sterile items.

2) Sterile items should not be exposed to air, but should be stored in a sterile bag or sterile container, and the name of the article and the sterilization date must be marked on the outside of the sterile bag, and they should be discharged in the order of expiration date.

3) Regularly check the sterilization date and storage of sterile items. Sterile packs generally have a shelf life of 7 days when they are not contaminated, and should be re-sterilized after expiration or moisture.

Extended Material: The Concept of Aseptic Technique:

1.Aseptictechnique refers to the operation technology and management method that prevents all microorganisms from invading the body and keeps sterile goods and sterile areas free from contamination in the process of performing medical and nursing technology.

2.AsepticsupplyAfter sterilization by physical or chemical methods, the articles that are not contaminated are called sterile articles.

3.The aseptic area is the area that has been sterilized and is not contaminated, which is called the sterile area.

4.Non-asepticareaA non-sterile article or area that has not been sterilized or contaminated after sterilization, is called a non-sterile article or area.

5.Relative Sterile Area (relativeasepticarea) Relative to the liter sterile area, it means that once the sterile item is taken out of the sterile container, it is considered to be relatively sterile and cannot be put back. The edge of the sterile zone is 3cm inward, which is the relative sterile zone.

6.InfectantContaminated items refer to items that have not been sterilized, or have been contaminated after sterilization.