How to write the period verification of the biosafety cabinet for everyone

The biosafety cabinet needs to be checked for normal indicators before use, including the following aspects:

1. Inlet air flow direction and wind speed detection

The inlet air flow direction is detected in the working section by the smoke generation method or the silk line method, and the detection position includes the surrounding edge and the middle area of the working window; The wind velocity of the inlet air flow is measured by an anemometer.

2. Detection of wind speed and uniformity of the downdraft

The anemometer is used to measure the cross-sectional wind speed with uniform distribution.

3. Cleanliness testing of the work area

Dust particle timer is used to detect in the work area.

4. Noise detection

Noise is measured with sound levels at the horizontal center of the front panel of the biosafety cabinet 300mm outward and 380mm above the work surface.

5. Illuminance detection:

Measuring points are set every 30 cm along the centerline along the length of the work surface.

6. Box leakage detection:

The safety cabinet is sealed and pressurized to 500 Pa, and after 30 minutes, a pressure gauge or pressure sensor system is connected to the test area for testing with the pressure decay method, or with the soap bubble method.

In order to ensure the safety of the biosafety cabinet, the safety cabinet should be maintained and maintained regularly:

1. The safety cabinet work area should be cleaned and disinfected before and after each use.

2. After the service life of the HEPA filter expires, it should be replaced by professionals who have received special training for biological safety cabinets.

3. The laboratory biosafety manual issued by the WHO, the American biosafety cabinet standard NSF49 and the China Food and Drug Administration biological safety cabinet standard YY0569 all require that the safety of the biological safety cabinet should be tested under one of the following conditions: before the installation is completed and put into use; annual routine testing; When the safety cabinet is displaced; Replace the HEPA filter and internal parts after repair.

The above content refers to: Encyclopedia - Biological Safety Cabinet.

The biosafety cabinet needs to be checked for normal indicators before use, excluding noise. The operating procedures used in the biosafety cabinet are as follows:

1 Before each use, the normal indicators of the biosafety cabinet should be checked, including wind speed, air flow, and negative pressure within the normal range. If there is an abnormality, it should be stopped and overhauled.

2 The glass observation window cannot be opened during use.

3. Minimal equipment or samples should be placed in the biological safety cabinet so as not to affect the airflow circulation of the rear pressure exhaust system.

4 Alcohol lamps are not allowed in the safety cabinet of Shenghui lifts, and miniature electric heaters are allowed, and disposable sterile inoculation loops are preferred.

5 All work must be carried out in the middle and rear of the work surface and be visible through the glass viewing baffle. The operator should not move and wave his arms frequently, so as not to disrupt the directional airflow.

6 Minimize movement behind the operator and quickly open and close the door to the room to prevent interference with the airflow of the biosafety cabinet. 7. Waste bags and containers for discarded straws must be placed in the safety cabinet and should not be placed outside the safety cabinet. Contaminated straws and containers should be disinfected in a container with disinfectant in a safety cabinet for more than 1 hour before they can be transferred to a special garbage bag for medical waste for autoclaving.

8. Clean items and used contaminated items should be placed separately in different areas, and the operation on the work surface should be carried out in the direction from the clean area to the polluted area to avoid cross-contamination.

9. Before and after the start and end of the work, the fan of the safety cabinet should run for at least 5 minutes, and the surface of the biological safety cabinet should be wiped with an appropriate disinfectant after the work is completed.

10 Paperwork is not allowed in the biological safety cabinet.