Gentian violet stains pasteur bodies at the same time, does not stain X chromosomes

The chromosomes contain proteins, and the purple reaction occurs when it encounters gentian violet, which has nothing to do with alkalinity, and the chromosomes are not acidic, and magenta acetate is alkaline.

High school biology textbooks describe chromatin and point out that chromatin (body) is easily stained dark by basic dyes. In the experiment, the chromatin (body) must be stained with alkaline dyes such as gentian violet solution or magenta acetate solution, which are prepared by dissolving gentian violet or magenta acetate in acetic acid solution, and the pH value of the prepared gentian violet solution is about 7 (acidic).

So why is gentian violet solution called an alkaline stain?

As a dye, it must meet two conditions: one is to have color; The second is to have an affinity with the infected tissue. The color of the dye and its affinity with tissues are determined by the molecular structure of the dye itself, and the chromophore that produces the color and the co-chromophore that produce affinity with the tissue jointly determine the dyeing properties of the dye.

As a dye substance, in addition to having a chromophore, it is also necessary to have a color-boosting group that ionizes the compound. For example, dye compounds often form chromogenic groups from nitro (-NO2), azo groups (-n=n-), vinyl groups, etc., and acidic groups such as -OH, -SO3H, and -COOH and basic groups such as -NH2, -NHCH3, and -N(CH3)2 form color-supporting groups. Their presence ionizes the dye substance, enhances the polarity, and promotes the interaction between the dye and the tissue to produce a dyeing effect.

We refer to dyes with acidic or basic groups in the co-color groups as acidic or basic stains, respectively.

For example, nitro is a chromogenic group, and when the 3 hydrogen atoms in the benzene ring are replaced by 3 nitro groups, it becomes a yellow compound of trinitrobenzene. Trinitrobenzene is not a dye, it has only one chromophore, it is not soluble in water, it cannot be ionized, it is neither acid nor alkali, and it cannot form salts with acids or bases. If the trinitrobenzene molecule replaces a hydrogen atom with a hydroxyl group, it becomes a trinitrophenol, that is, picric acid, which is a yellow dye with ionization and strong alkali to form salts, and the hydroxyl group here is the color-supporting group.

It can be seen that the color of picric acid is caused by the chromogenic group (nitro), and its dyeing performance is formed by the chromophore group (hydroxyl group), if the amino group is used instead of the nitro group, it will form a colorless compound, not a dye. It can be seen that as a dye, there must be a chromophore and a color-booster group to cooperate with each other, and both are indispensable.

For example, eosin Y contains a (-Cooh) co-color group that releases hydrogen ions when ionized in water, which itself has a negative charge. When preparing eosin Y dye, it reacts with the strong alkali NaOH to form salt (-coona), and the Na+ of this substance is alkaline in solution, so it cannot be considered that the acid dye is acidic in solution.

Therefore, the definition of acidic (basic) dyes is not determined by the pH of the dye solution, but by the charge carried by the ionization of the co-color groups in the dye substance. In general, positively charged dyes with colorants are alkaline stains, and vice versa are acidic stains.

Pap bodies are observed in interphase, and other chromosomes, including another X chromosome, are in chromatin state.

Alpha violet, synonyms: gentian violet, crystal violet, the carrier chromosome of DNA can be stained by sex dye, because the proteins that make up the chromosome have the property of being stained, hence the name. The coloring part of gentian violet stain solution is cationic, which easily replaces H on the molecule and colores the substance, so it can react with the proteins that make up the chromosomes to make it color.

Chromatin and chromosomes are the same in nature, and they are the name of the same substance in different periods, so they can be stained by gentian violet.

Q: Gentian violet and magenta acetate are chromatin-staining and are both basic dyes.

Chromosomes and chromatin are essentially one thing.

No, they're chemical agents.

1. D gentian violet is an alkaline dye, these dyes are prepared by dissolving gentian violet or magenta in acetic acid solution, and the pH value of the prepared gentian violet solution is about 7 (acidic). So why is gentian violet solution called an alkaline stain?

This is because the definition of acidic (basic) dyes is not determined by the pH of the dye solution, but by the charge carried by the ionization of the co-color groups in the number of dye-substances.

Since the pH is less than 7, it can only be combined with alkaline, and in the chromosomes it is the amino group in the alkaline potato.

2. The totipotency of animal culture-resistant cells is not easy to express, while plant cells have totipotency, while seed coat cells are highly specialized cells with a thick cuticle, and are generally dead cells.