Who knows how many sizes of Coagulate Whole Blood Genomic DNA Extraction System are available and ho

There are two main specifications.

DN3101 160 times 50 L with protease K) element DN3102 320 times 50 L with protease K) meta-product introduction: This kit according to the characteristics of coagulation whole blood exclusively developed nuclear lysate with protease K to cleave coagulated blood clots to release genomic DNA, and then the protein precipitate selectively precipitates to remove the protein, and finally the pure genomic DNA is precipitated by isopropanol and redissolved in DNA lysis solution under the help of Sigma's molecular biology grade glyc ogen.

Features:1There is no need to use reagents such as toxic phenol.

2.Fast and simple, a single sample can be completed in less than 1 hour.

3.The results are stable and cooperativeSigma's molecular biology grade glycogen helps precipitate trace amounts of DNA with high yields, with typical ratios of OD260 to OD280 and lengths of 50kb-150kb, which can be used directly for constructing texts.

Library, PCR, Southern-Blot, and various digestion reactions.

Model: DN3101

Specification: 50 l for 160 times

**: 560 660 (with proteinase K).

Model: DN3102

Specification: 50 l for 320 times

**: 1040 1240 (with proteinase K).

Coagulated whole blood genomic DNA extraction system specification catalog from Blinko!

Brine's product introduction:

This kit uses high-efficiency red blood cell lysate, first remove red blood cells without nucleus in the blood and centrifuge ** white blood cells, interpret genomic DNA through nuclear lysate, silica membrane can specifically adsorb genomic DNA, remove proteins and other acrobatics after rinsing, and finally elute with low-salt solution to obtain high-purity genome.

dna。In the process of experiment, there is no need for toxic organic solvents such as phenol and chloroform, and there is no need for steps such as ethanol precipitation, which takes a shorter time and the purity of the extracted DNA is more high, which is applicable.

in PCR, enzyme digestion and other downstream experiments.

Kit Features:

The imported silica gel membrane is used, the genomic DNA yield is high, and the experimental results are reproducible.

u do not use toxic phenol and other reagents, do not need ethanol sink, mountain lake and other steps;

Fast, simple, and high-purity genomic DNA can be obtained within one hour of a single sample;

The Medium Mass Bacterial Genomic DNA Extraction Kit is still very effective, and can be used to quickly extract genomic DNA from various bacteria. After the bacterial sample is added to the nuclear lysate (or lysozyme or some other enzyme to help lyse the cell wall), the cells are first lysed under the action of a strong detergent to release the basal hollow ridge kinetogen DNA, then RNA SE A is added to remove the RNA, then the protein precipitate is selectively precipitated to remove the protein, and finally the pure gene field panel DNA is precipitated by isopropanol and redissolved in the DNA lysate.

Product features: 1. There is no need to use toxic phenol and other reagents.

2. Fast, simple, a single sample operation can generally be completed within 30 minutes.

3. The results are stable and the yield is high, the typical ratio of OD260 OD280 is up to 50 kb -150kb, and the length can be up to 50 kb -150kb, which can be directly used for library construction, PCR, southern-blot and various enzyme digestion reactions.

The Viral Genomic DNA RNA Extraction Kit can be used to purify viral DNA or viral RNA from plasma, serum, and cell-free body fluids using a separation column method.

Using a high-efficiency, specific nucleic acid-binding centrifugal adsorption column and a unique buffer system, it is suitable for isolating and purifying viral DNA or RNA from plasma, serum and other cell-free body fluids. The obtained viral genomic DNA or RNA is not contaminated by proteins, nucleases or other impurities, and can be directly used in molecular biology experiments such as PCR, enzyme digestion, hybridization, and reverse transcription.

The kit is simple and fast to operate, and high-purity DNA or RNA can be obtained within an hour. It is used for the extraction of various viral DNA RNA nucleic acids, such as HCV, HIV, HPV, animal pathogenic viruses, etc.

Features: Rapid purification yields high-quality viral DNA and RNA.

No organic extraction or ethanol precipitation.

High repeatability and high yield.

Complete removal of contaminants and inhibitors for easy downstream application.

Downstream applications. Viral genotyping studies

Epidemiological studies of viruses.

Infectious disease research.

Storage conditions: Room temperature.

The Blood Genomic DNA Extraction Cassette for Blinker uses an adsorbent column method to extract high-purity genomic DNA from blood.

This kit is a well-developed genomic DNA extraction kit specifically for blood samples, suitable for genomic DNA extraction of whole blood treated with various anticoagulants (sodium citrate, EDTA, etc.), and also suitable for genomic DNA extraction of blood clots. The kit uses a unique cell lysis and decomposition system to denature the protein, and combines the principle of silica gel membrane specific adsorption of nucleic acids to quickly purify genomic DNA. The DNA purified by this kit is free of protein, nuclease and other contamination, and can be directly performed for PCR, enzyme digestion and Southern hybridization.

Features: The unique Liangchang cell lysis system can directly extract genomic DNA from whole blood, acorn white coat, blood clots and other samples.

Using the principle of silica gel membrane adsorption, high-quality DNA can be obtained 30 minutes after loading the adsorption column.

Complete removal of contaminants and inhibitors for downstream applications.

Storage conditions: Room temperature.

Brianco is a medium whole blood genomic DNA extraction kit (3ml) that extracts genomic DNA from several rapid steps according to the characteristics of whole blood. First, the red blood cell lysate lysate lyses to remove the red blood cells that do not contain DNA, the nuclear lysate lyses the leukocytes to release genomic DNA, then the protein pellet selectively precipitates to remove the protein, and finally the pure genomic DNA is precipitated by isopropanol and redissolved in the DNA lysate.

Kit Features:

Optimized red blood cell lysate formulations from more than a dozen formulations for rapid and complete lysis.

There is no need to use reagents such as toxic phenol.

Fast and simple, a single sample can be completed in less than 1 hour.

The results are stable, the yield is high (75-150 g can be extracted from 3 ml of whole blood in a typical yield), the purity is up, and the length can reach 50 kb-150 kb, which can be directly used to construct libraries, PCR, Southern-blot and various enzyme digestion reactions.

Liquid's kit composition.

Save. 20 times.

50 times. dp2101）

dp21002）

Red blood cell lysate. Room temperature.

ml of nuclei lysate. Room temperature.

mlml protein pellet.

The room is noisy and warm.

mlmlDNA lysis solution. Room temperature.

MLML This kit is stored at room temperature for 18 months without affecting the use effect.

The main components of the small whole blood genomic DNA rapid extraction kit are:

Buffer BB

Conjugate solution CB

Inhibitor removal solution IR

Rinse solution, WB elution buffer, EBEB

Adsorption column AC

Collection tube (2 ml).

Product features: 1. There is no need to use toxic phenol and other reagents, nor does it require steps such as ethanol precipitation.

2. Fast, simple, a single sample operation can be completed within 20 minutes.

3. Multiple column rinsing to ensure high purity, the typical yield of 200 L whole blood can extract 3-6 g, the typical ratio of OD260 OD280 is up, the length can reach 30 kb -50kb, and can be directly used in PCR, Southern-blot and various enzyme digestion reactions.

4. The red blood cell lysate formula selected from more than a dozen formulas is fast and complete for lysis, and customers can choose to purchase it according to their needs.

A typical yield of 200 L whole blood yields 3-6 g of genomic DNA.

5. The eluent EB does not contain the chelating agent EDTA, which does not affect the downstream enzyme digestion, ligation and other reactions. Water can also be used for elution, but it should be ensured that the pH of the batch is greater than that, as too low a pH affects the elution efficiency. Eluting DNA with water should be stored at 20.

DNA can be eluted with TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH) if long-term storage is required, but EDTA may affect downstream digestion reactions and can be appropriately diluted when used.

Blinko is a very useful DNA extraction kit.

This kit uses a centrifugal adsorption column and a unique buffer system that can efficiently and specifically bind DNA, which is suitable for the extraction of genomic DNA from gram-negative bacteria and the genomic DNA extraction of gram-positive bacteria. This kit can also be used for genome extraction of food pathogenic bacteria (microorganisms), such as: Staphylococcus aureus, Vibrio cholerae, hemorrhagic Escherichia coli O157:

H7, single increase listeria, Salmonella, sakazaki enterotobacter, etc. 10-40 g of pure genomic DNA can be quickly extracted and purified from 1-5ml of bacterial culture medium, and the obtained genomic DNA can be directly used as PCR template, enzyme digestion, hybridization and other molecular biology experiments.

Product Applications. Gram-negative bacteria genome extraction.

Genome extraction of gram-positive bacteria.

Genome extraction of pathogenic bacteria in food.

Features: Simple and fast: Gram-negative bacteria can obtain highly pure genomic DNA in less than 1 hour.

:d RNA can be directly used in molecular biology experiments such as PCR, enzyme digestion, and hybridization.

Storage conditions: Room temperature.

This is not clear, we are using the KingMcGonagall kit, the magnetic bead method, and now the column lift is outdated, right?