What is MH medium What bacteria are suitable for MH medium

MH medium is MH agar medium, and MH referred to in microorganisms is an acronym for Mueller-Hinton, which is hydrolyzed casein. Therefore, MH medium also becomes hydrolyzed casein medium.

The principle of MH medium: beef meal and acid-hydrolyzed casein provide nitrogen sources, vitamins and amino acids; Soluble starch absorbs toxic metabolites.

mhCulture mediumSuitable for E. coli, E. coli is red or pink on it, and some colonies are only red in the middle.

Some actinomycetes and viruses are generally not cultured, for example, Actinomycetes are mostly used in Gao.

No. 1 synthetic medium, viruses are mostly cultured with chicken embryos.

Introduction to MH medium:MH medium is MH agar medium, and MH referred to in microorganisms is an acronym for Mueller-Hinton, which is hydrolyzed casein. Therefore, MH medium also becomes hydrolyzed casein medium.

The principle of MH medium: beef powder and acid hydrolyzed casein provide nitrogen sources and vitamins.

and amino acids; Soluble starch absorbs toxic metabolites.

The above content refers to Encyclopedia-MH agar medium.

MH in microorganism is an acronym for Mueller-Hinton, which refers to hydrolyzed casein.

The MH medium refers to the hydrolyzed casein (Mueller-Hinton) medium, which is the standard medium for aerobic bacteria and facultative anaerobic bacteria susceptibility test adopted by the NCCLS National Clinical Laboratory Standards Committee, with an agar thickness of 4mm.

MH medium is MH agar medium, and MH in microorganisms is an acronym for Mueller-Hinton, which refers to hydrolyzed casein. So the MH medium also becomes the hydrolyzed casein medium. It is often used as a raw material for culture media to provide the growth factors needed for bacterial growth.

Principles of MH medium:

Beef meal and acidolyzed casein provide a source of nitrogen, vitamins and amino acids; Soluble starch absorbs toxic metabolites.

Composition of MH medium:

Acid Hydrolyzed Casein Acid Hydrolyzed Casein.

Beef extract powder 2g

Starch agar 12 g

Usage of MH medium:

Weigh this product and heat it to dissolve in 1000mlDistilled water, 121 autoclave for 15 min, set aside.

Quality control of MH media:

Quality control is the determination of the inhibition ring of the quality control strain using the same operation method as in the conventional experiment. Fresh passage cultures should be used. The coating method of inoculation solution is the same as that of conventional operation, and the type of antimicrobial measured should be the same as that of conventional measurement.

Apply the broth. In general, the medium in the heated medium is the crude microorganisms for the side. The medium is prepared due to the different raw materials and nitrogenous substances.

Since the liquid medium is not easy to store for a long time, potato juice and other natural ingredients are prepared. Some media that need to be strictly sterilized (such as tissue culture medium), called synthetic medium or comprehensive medium, called natural medium, inorganic salts (including trace elements), vitamins and water, etc., are easy to be contaminated or decomposed by bacteria. Culture medium in chemical reagents.

Some media also contain antibiotics and pigments, which must be stored in a cool place in 2 6, and are now transformed into powders.

According to the different raw materials used, and the storage and storage are also slightly not transported to the same source, protected from light, can be divided into two categories, so the general medium must be moisture-proof, longer storage. c. The refrigerator, the artificial nutrients for the growth and maintenance of plant and animal tissues, after moisture absorption, generally contain carbohydrates; Most of the stools that are formulated with chemicals and labeled with ingredients are synthetic mediums, and the requirements for use are different.