What are the components of DMEM medium

DMEM is Dulbecco'S Modified Eagle Medium is an abbreviation that contains a variety of amino acids.

and glucose medium, which is developed on the basis of MEM medium. Compared with MEM, the dosage of various ingredients is increased, and it is divided into high sugar type (less than 4500 mg L) and low sugar type (less than 1000 mg L). The high glucose type is conducive to the growth of cells in one location, which is suitable for tumor cells that grow faster and are difficult to attach.

Wait. DMEM medium.

DMEM is a type of glucose that contains various amino acids and glucose.

The medium is developed on the basis of MEM medium. Compared with MEM, the dosage of various ingredients is increased, and it is divided into high sugar type (less than 4500 mg L) and low sugar type (less than 1000 mg L). The high glucose type is conducive to the growth of cells in one location, and is suitable for tumor cells that grow faster and have difficulty attaching to them.

DMEM is Dulbecco'S modified Eagle Medium, so its features mainly include the following:

1) The amino acid content is twice that of Eagle's medium, and it contains non-essential amino acids, such as glycine.

Wait. 2) The vitamin content is 4 times that of Eagle's medium;

3) Contains glycolytic pathways.

pyruvate.

4) Contains trace amounts of iron ions.

DMEM is a kind of wild judgment wisdom containing various amino acids.

and glucose medium.

It is developed on the basis of MEM medium. Compared with MEM, the dosage of various ingredients was increased, and it was divided into high sugar type (higher than 4500 mg L) and low sugar type (less than 1000 mg L). The high glucose type is conducive to the growth of cells in one position, and is suitable for tumor cells that grow faster and have difficulty in attaching to Songchongpei.

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DMEM is Dulbecco'The abbreviation of S Modified Eagle Medium is a medium containing various aminophadactyl base acids and glucose, which is developed on the basis of MEM medium. Compared with MEM, the dosage of various ingredients is increased, and it is divided into high sugar type (less than 4500 mg L) and low sugar type (less than 1000 mg L). The high glucose type is conducive to the growth of cells in a high disorderly position, and is suitable for tumor cells that grow faster and have difficulty attaching to tumor cells.

DMEM is Dulbecco'The abbreviation of S Modified Eagle Medium is a medium containing various amino acids and glucose, which is developed on the basis of MEM medium. Compared with MEM, the dosage of various components was increased, and at the same time, it was divided into high-sugar type (less than 4500 mg L) and low sugar type (less than 1000 mg L).The high glucose type is conducive to the growth of cells in one position, and the suitable for the growth of tumor cells that grow faster and are more difficult to attach to.

Therefore, its Tenaichang points mainly include the following:

1) The amino acid content is twice that of Eagle's medium, and it contains non-essential amino acids, such as glycine, etc.;

2) The vitamin content is 4 times that of Eagle's medium;

3) Contains pyruvate, an important substance in the glycolytic pathway;

4) Contains trace amounts of iron ions. Eagle comes from Harry Eagle, the inventor of this medium

DMEM is a medium containing various amino acids and glucose, which is developed on the basis of Mori brother early in MEM medium.

DMEM-Low Glucose (Standard) is a widely used medium that can be used in many mammalian cell cultures. Low sugar is suitable for dependent adherent cell culture, suitable for tumor cell culture with fast growth rate and poor attachment.

DMEM-High Glucose (Standard) is a widely used medium that can be used in many mammalian cell cultures, and is more suitable for high-density suspension cell cultures. It is suitable for clonal culture with poor attachment but does not want it to detach from the original growth point, and can also be used for the culture of myeloma cells and DNA-transfected transformed cells in hybridomas.

DMEM F12 medium is suitable for clone density culture. F12 medium is composed of dust and contains a variety of trace elements, which binds to DMEM 1:1, which is called DMEM F12 medium (DME F12medium).

As a basis for the development of serum-free formulations, the medium is suitable for mammalian cell culture at low serum levels. In order to enhance the buffering capacity of this medium, one of the modifications is to add 15 mM Hepes buffer to DMEM F12 (1:1).