How to maintain a refrigerated centrifuge

Precautions for daily use 1 The centrifuge should be placed on a flat and firm ground or on a laboratory bench, and tilting is not allowed; 2 Usually the centrifuge will have a registration form, please register the user, rotor, speed and time before use; 3. The centrifuge tube must be balanced with a balance (weight balance), cover the lid of the centrifuge tube and screw it tightly; 4. Put the balanced centrifuge tube symmetrically into the centrifugal rotor (position balance), close the lid of the centrifugal rotor, and pay attention to whether it is tightened; 5. When the centrifugation is completed, it is necessary to wait for the centrifuge to stop automatically, and it is not allowed to force the centrifuge to stop with hands or other objects, and the cabin can be opened only after the rotor is completely stationary.

Precautions for use.

1) Usually the centrifuge will have a registration form, please register the user, rotor, speed, and time before use.

2) The centrifuge tube must be well balanced, and the position should also be balanced when putting in the centrifugal top. Never exceed the maximum speed limit of the centrifuge or centrifugal rotor.

3) Be sure to reach the preset speed before leaving the centrifuge; If there is any abnormality, stop the machine immediately.

4) Usually you can know whether the centrifugation condition is normal by listening to the sound, and you can also pay attention to the vibration of the centrifuge.

5) After using samples of high-salt solutions such as ammonium sulfate, be sure to wash the centrifugal top and clean the centrifuge transfer compartment.

6) Ultracentrifuges are more complex due to their extremely high speeds, requiring additional training before they can be used.

What is the use, the filter should be checked frequently.

1 Check the instrument.

2 Select the rotor.

3 Turn on the power and turn on the power switch.

4 Load the liquid to be centrifuged into a suitable centrifuge tube and place it symmetrically into the rotor head.

5 Adjust the speed and time.

6 Turn on the switch.

7. After centrifugation, it will automatically shut down, turn off the freezer switch, power switch, and cut off the power supply.

8 Take the rotor out and leave the lid of the centrifuge open.

9 Collect the centrifuge and wash the centrifuge tube.

Refrigerated centrifuge is mainly for high-speed centrifugation, to prevent some centrifugal substances (such as proteins, etc.) from producing qualitative changes at high temperatures, if you need a centrifuge, you can go to Shanghai Zhao Di Biotechnology** to consult their technicians, we have purchased instruments in the laboratory are consulted at their homes, I hope it will help you.

Refrigerated centrifuges separate liquids through different freezing points.

In order to maintain biological activity (sample) while centrifugation.

1.Centrifuge maintenance.

Clean the rotor chamber and rotor with water or mild detergent, and do not use alkaline solutions or solvents that are abrasive to the material.

Use a rag or tweezers to remove the swag debris from the rotor chamber.

Open the top lid when the centrifuge is not in use and keep the rotor chamber dry to avoid wear on the motor bearings.

Special safety precautions must be taken when the samples are toxic, radioactive, or contaminated.

2/62.Maintenance of centrifuge accessories.

If the centrifuge tube shows color change, deformation, leakage, etc., it must be discontinued.

Do not screw on the cap when sterilizing the centrifuge tube at high temperature and high pressure to avoid deformation of the tube. The temperature tolerance for each tube is listed in this section of the user manual.

The centrifuge should be kept at a certain distance from other electrical equipment as much as possible, and there should be good grounding measures and regular inspections.

Centrifuges and rotors must not be exposed to high-intensity UV radiation or prolonged heat. Apply a neutral detergent when cleaning.

The rotor can be replaced if required. After reinstalling, tighten the rotor screws.

3/63.Rotor pins.

The rotor pins need to be lubricated frequently with lubricating oil to ensure the smooth operation of the centrifuge.

4/64.Maintenance of the condenser.

The condenser is used to cool the refrigerant and is installed at the rear of the centrifuge and is air-cooled.

The condenser should be cleaned of dust regularly to avoid obstruction of heat transfer. This can be cleaned with compressed air.

5/65.Sterilization and disinfection of the rotor chamber and accessories.

All conventional disinfectants can be used. Since centrifuges and accessories are made of different materials, the compatibility of disinfectants must be taken into account.

6/66.Operational checks.

The operator should ensure that the important parts of the centrifuge are in good condition, mainly referring to:

1.The motor suspension is stable.

2.There is no deviation of the hinge.

3.The rotor and accessories are free of corrosion.

4.Screw connection fastening.

In addition, the ground wire must be checked regularly.

Depending on the type of machine you have, whether it is a tubular centrifuge or a decanter centrifuge or a three-legged centrifuge, different machines will definitely be different.

The easiest way is to read the manual several times, in the manual there will be not only operating instructions and daily maintenance, etc., if the manual is missing, you can contact the manufacturer to let them express a manual or directly give you an electronic version of the e-mail. Some common faults are generally guided in the operation training after the purchase, if the operator at that time is changed, you can consult the manufacturer, it is best to contact them for another training.

You can read the instructions

1. The centrifuge must be operated in strict accordance with the instruction manual, and the dryness and hygiene of the instrument must be ensured before and after each use;

2. Clean the centrifugal chamber with neutral detergent and dry it with a soft cloth in time after using the high-sensitivity heart machine, and it is not allowed to scrub the rotor with non-neutral detergent to prevent chemical corrosion of the inner cavity.

3. If it is not used for a long time, clean the centrifuge chamber, and avoid detergent and water pouring in the centrifugal chamber to prevent the centrifuge motor from rusting and reducing its service life, and open the door cover to dry.

4. The centrifuge should be stored indoors, the ground should be flat, the air should be free of conductive dust, no corrosive or insulation-destroying gas, and there should be no other strong vibration sources nearby, and the site should have ventilation devices and drainage devices to improve the life of the whole machine.

High-speed refrigerated centrifuge operation steps.

1.Turn on the power switch of the desktop high-speed refrigerated centrifuge and enter the standby state.

2.Choose the right rotor: the liquid contained in the centrifuge tube can not exceed 2 3 of the total capacity when centrifuging, otherwise the liquid is easy.

in overflow; Before and after use, pay attention to whether there is any leakage liquid residue in the rotor and keep it dry. Conversion.

When rotating, care should be taken to make the bayonet of the centrifuge shaft and rotor firmly stuck. The centrifuge tube equilibration error should be within grams.

3.Select centrifugation parameters:

a) Press the temperature setting button, then use the number key to set the centrifugal temperature, and press enter to confirm.

b) Press the speed setting button to switch between RPM RCF setting gears, use the number keys to set the centrifugal speed, and press enter to confirm.

c) Press the rotor setting button, then use the number key to set the rotor model, and press enter to confirm.

d) Press the time setting button, then use the number key to set the centrifugation time, and press enter to confirm.

e) The braking or acceleration speed of the centrifuge is generally set between 0 4 and should not be adjusted frequently.

4.Place the balanced tube symmetrically into the rotor. Tighten the screws on the lid with a turn head.

5.Press the centrifuge cover door, if the lid door is not securely closed, the centrifuge will not start.

6.Press the start button to start centrifugation. After the centrifugation starts, you should wait until the centrifugation speed reaches the set speed before leaving.

Once you find that the centrifuge is abnormal (such as unbalanced machine vibration or loud noise), you should immediately press the stop button, if necessary, directly press the power switch to cut off the power supply, stop centrifugation, and find out the reason.

8.After use, please clean the rotor and centrifuge chamber, and do not close the centrifuge lid to facilitate the evaporation of moisture.

9.At the end of use, it must be registered and the use of the use must be indicated.

Precautions for high-speed refrigerated centrifuge operation.

1.When the desktop high-speed refrigerated centrifuge is pre-cooled, the centrifuge cover must be closed, and the rotor should be placed upside down on the laboratory bench after centrifugation, and the residual water in the cavity should be wiped dry, and the centrifuge cover should be opened.

2.When ultracentrifugation, the liquid must be filled with the centrifuge tube, and the vacuum should be evacuated when it should be overrun, and only by filling up can the centrifuge tube be deformed. If the lid of the centrifuge tube is not tightly sealed, the liquid cannot be filled to prevent spillage, which will affect the normal operation of the sensor.

3.When the rotor is pre-cooled, the rotor cover can be placed on the platform of the centrifuge or placed on the laboratory bench, and it must not be tightened and floated on the rotor, because once it is started by mistake, the rotor cover will fly out and cause an accident!

4.After tightening the rotor cover, be sure to touch the gap between the rotor and the lid with your fingers, if there is a gap, unscrew it and re-tighten it until it is confirmed that there is no gap before starting the centrifuge.

5.Be sure to ground the wire when using. The substances added to the centrifuge tube should be relatively balanced, if the two sides are unbalanced, it will cause great damage to the centrifuge, at least it will shorten the service life of the centrifuge.

6.During the centrifugation process, the operator must not leave the centrifuge room, and once an abnormal situation occurs, the operator cannot turn off the power (power), and press STOP. Before pre-cooling, fill in the centrifuge usage record.

Usually after use, pay attention to clean the rotor, do not be the rotor produces hard injuries, such as low, etc., if in case of falling, to reduce X-ray production, confirm before it can be put into production, for the rotor life is almost to the life, should be replaced in time, Hengan production of centrifuge description said that there are about the centrifuge rotor hours and times, no matter which one expires first, it needs to be replaced.