How to clone a plant with one leaf???

The plant cloning method is also a tissue culture method, and the conditions required for culture are generally not reached. Because this requires sterile laboratories, production tools, culture media, culture flasks, distilled water, disinfectants, etc. It is a complex and high-tech growth procedure, and it is difficult to succeed without professional training and technology.

However, it is possible to reproduce using asexual reproduction, which is something that can be done by ordinary families or members. It can be propagated by cuttings, grafting, branching, and striping. The grafting method is mainly used in varieties that are more difficult to root; The dividing method is mainly used in varieties with outstanding tillering performance. The strip pressing method is also used for varieties that are more difficult to root; Only the use of cuttings is the most widespread and practical.

Generally, these asexual reproduction methods can ensure that the excellent traits of the plant will not be mutated.

There are four forms of cuttings: leaf cuttings, branch cuttings, bud cuttings, and root cuttings. Leaf cuttings take African violets as examples; Choose strong and disease-free leaves, if the petiole is too long, you can cut off a section, generally leave 3-5 cm, pierce a deep 2 cm hole in the substrate, insert the leaf into the hole, and then gently press the solid with your fingers, the temperature is controlled at 15-25, 10-20 days of petiole rooting, about 50 days of small buds to grow, when the small buds stretch 3-5 leaflets, cut them off and plant them in pots, you can also insert the leaves into clear water, pay attention to only make the petiole into the water, about 1 month can take root into seedlings.

The bud cuttings take chrysanthemums as an example: in spring, select the strong buds on the side of the pot as cuttings, the cuttings are about 5 cm long, remove the lower leaves, leave only the upper 2 young leaves, insert about 1 3-1 2 into the substrate, put it in a cool place to moisturize after drenching enough water, spray 1-2 times a day, but the water can not penetrate into the substrate. It is advisable to keep the temperature between 15-25.

After 2 weeks, it can be placed in a sunny place to facilitate rooting, and after another 2 weeks, it can be rooted and potted, and the potting time is the highest survival rate when the root system grows at 3-4 cm.

The branch cuttings take willow trees as an example: before the branches germinate in early spring, cut the one- and two-year-old robust branches with a length of about 10 cm, and quickly put them in clean water to avoid the cutting mouth entering the air and affecting the rooting. The lower part of the cut should be beveled, which is conducive to inserting into the matrix, the insertion depth should be 1 3-1 2, and the strip spacing should be about 5 cm.

After pouring through the water, the cover film is insulated and moisturized, and the substrate should be hydrated when the dryness reaches 8 percent. The temperature is kept around 20-25 for the fastest growth, and transplantation can be done after 1 month.

The root cutting method takes African jasmine as an example: there are many roots exposed on the edge of the African jasmine pot in the old pot, and it is directly buried in the substrate after being cut with sharp scissors, and the depth of burial is just enough to be buried, and the covering soil should not be too deep, otherwise it is not conducive to budding. It is advisable to keep the temperature between 20-30 and place it in a semi-humid environment, and then plant it in 2 months.

When planting, it can also be planted after shortening according to the rooting situation of the root segment. However, the new plants after planting should be carefully maintained, first placed in a semi-shaded and humid place, and then watered the second time after the first watering is dry, and gradually transition to a sunny place, but avoid direct sunlight in the high temperature season.

You can choose begonias.

Example 1: "Dolly" sheep: In August 1996, scientists extracted somatic cells from a black-faced sheep, and then injected the nucleus of this somatic cell into the egg cell of another white sheep, and the egg cell had been removed from the nucleus, and finally the newly synthesized egg cell developed in the womb of the third black-faced sheep to form Dolly sheep.

Theoretically, Dolly inherited the genetic traits of the sheep that provided the somatic cells. The technique of breeding Dolly sheep has become the standard process for breeding somatic cloned animals today.

Example 2: The stem of a strawberry is creeping on the ground, and every time it grows, it will produce whisker-like roots that automatically burrow into the soil, and then cut off the stem to form a new plant.

Example 3: Mushrooms reproduce by spores emitted into the air, and the spores will grow when they attach to a suitable environment.

Bamboo, reed, and lotus have well-developed rhizomes, and side buds grow from the axils of the scaly leaves of the rhizomes, and then grow from the side buds into leaves with long stalks, and many adventitious roots grow at the base of the petioles. Each rhizome grows many new aboveground plant bodies each year.

Example 4: A willow tree can grow into a willow tree by breaking off its branches.

Plant cloning is vegetative propagation.

There are four main ways.

Divide the root one. Split roots: used for oleander, plum and other shrubs, their clumping stems each have roots, can directly separate them into independent plants. This type of reproduction is called root splitting.

Bead two. Striping: For mulberry, oleander and other plants, you can choose the longer branches on the tree, bend it down, and after the branches buried in the soil grow roots, then cut off the branches from the parent body to grow into new plants. This method is called a bead.

Cuttings three. Cuttings: Used for plants such as moon, willow, grapes, etc., you can cut the budding branches on the plant and insert them into the soil, and soon these branches will take root and sprout and grow into new plants. This method is called cuttings.

Graft four. Grafting: Used in fruit trees such as oranges and peaches, their branches or buds can be attached to the stems or roots of another plant, so that the cambium layer of the two (the tissue with meristem in the stem) is close together, and soon they will grow into one and become a new plant.

This method of reproduction is called grafting.

willows, cacti, mulberry, spider plants.

The most common is flower tissue culture.

--- many kinds of plants can be cloned, the cloning of plants is called "tissue culture", which is generally used to propagate some rare plants or plants with greater economic value, otherwise I am afraid that even the cost of tissue culture will not be recovered, the most common are "phalaenopsis" and "orchids".

1. Rapid propagation of some rare plants or plants with greater economic value Relying on natural conditions to propagate rare plants and plants with high economic value in a short period of time is limited by the geographical environment and season, and it is difficult to achieve the purpose of fast and efficient; Especially for plants that need to reach a certain number in a short period of time in order to create valuable plants, time is the benefit, and only through the method of tissue culture can this requirement be met.

The propagation of plants by tissue culture is the main and most effective example of the application of tissue culture to production. The first is the successful application on orchids. Since Morel obtained orchid tissue culture seedlings in 1960, they were quickly used in production, forming a tissue culture propagation orchid industry.

Because the obvious feature of tissue culture propagation of plants is that it is fast, and it can reproduce millions of times faster every year, it is of particular significance to the propagation of some famous and excellent plant varieties with low reproduction coefficient and cannot be propagated by seeds. 2. Detoxification Many plants carry viruses, which seriously affect the yield and quality of plants and bring disasters to agriculture. In particular, vegetatively propagating plants, such as potatoes, strawberries, garlic, carnations, etc., because the virus is transmitted through vascular bundles, the use of these plant vegetative organs to reproduce will bring the virus to new plant individuals and cause diseases.

However, it has also been shown that not every part of the susceptible plant carries the virus, such as the part of the stem tip where the growth point has not yet differentiated into vascular bundles, which may not carry the virus. If the stem tip culture is cultivated using tissue culture, it is possible to obtain virus-free seedlings for regenerated plants, and then use such seedlings for propagation, so that the planted plants will not or rarely develop viral diseases.

The obtained detoxified vaccine must be identified and confirmed to be virus-free before it can be used. The use of tissue culture to obtain detoxified seedlings has been successful in strawberries, grapes, carnations, etc., and has obvious economic effects.

3. Preservation of plant germplasm resources and saving endangered plants For a long time, people have thought of many ways to preserve plants, such as storing fruits, storing seeds, storing roots, tubers, bulbs, and bulbs; With normal temperature, low temperature, variable temperature, low oxygen, inert gas filling, etc., these methods have received good or relatively good results to a certain extent, but there are still many problems. The main problems are the high cost to pay, the large space occupied, the short storage time and the susceptibility to environmental conditions. Plant tissue culture combined with ultra-low temperature preservation technology can bring a big leap forward to plant germplasm preservation.

Because preserving a cell is equivalent to preserving a seed, but it only takes up 1/10,000 of the original space, and it can be stored for a long time in liquid nitrogen at -193 degrees, unlike seeds, which need to be renewed every year or often.

Any organism can be cloned, it just needs equipment and technology.

There are willows, potatoes, cacti, spider plants, onions.

Potato, willow, bacteria, cactus.

Willows, potatoes, cacti, ......

willows, potatoes, cacti, onions.