The method of culturing cells into new plants ex vivo is called

Cell culture media:

Zhejiang Weikang brand cell culture medium includes MEM medium, DMEM medium, DMEM F12 medium, RPMI1640 medium, MEM medium, IMDM medium, M199 medium, and MCCOY'S 5A medium, HAM'S F-10 medium, HAM'S F-12 medium, HAM'S F-12K medium and Leibovitz'SL-15 medium, etc.

2.Disposable ready-made sterile medium with less nutrient loss and ready to use right out of the bottle.

3.Verified by multiplex cell testing, the culture quality is reliable, and the variability between batches is small.

4.In stock**, a variety of component combination cell culture media can be customized according to customer needs.

5.Filter membrane filtration sterilization, no pollution; Formulated with water for injection and low endotoxin.

Zhejiang Weikang Medical Technology Co., Ltd. is committed to developing a high-quality, effective and stable basic cell culture medium, providing suitable pH, osmotic pressure and nutrients for cell growth, so that cells can maintain a good state in an artificial simulation environment in vitro. Cell basal medium series products include MEM medium, DMEM medium, DMEM F12 medium, RPMI1640 medium, MEM medium, IMDM medium, M199 medium, and MCCOY'S 5A medium, HAM'S F-10 medium, HAM'S F-12 medium, HAM'S F-12K medium and Leibovitz'SL-15 medium, as well as a variety of customizable media, are suitable for in vitro culture of a variety of suspended and adherent mammalian cells and cell lines.

In vitro culture, also known as plant tissue culture, refers to the isolation of tissues that meet the needs from plants. Organs or cells, protoplasts, etc., are cultivated under artificially controlled conditions through aseptic manipulation to obtain regenerated intact plants or other products of economic value. The concept of plant tissue culture (in the narrow sense) refers to the use of various parts of the plant to organize, such as the cambium layer.

Parenchyma. Mesophyll tissue. The endosperm is cultured to obtain regenerated plants, which also refers to the culture of calli from various organs in the process of culture, and the callus is then redifferentiated to form regenerated plants.

Plant tissue culture techniques.

Direct pathway: refers to the direct induction and differentiation of somatic embryos from embryonic cells in some parts of the explant, such as cotyledons, inflorescences, beads and other explants.

Indirect pathway: explants first dedifferentiate to form callus, and then some cells of the callus are re-"determined" to embryonic cells, and then differentiated into somatic embryos. Most somatic embryos are formed through this pathway. It usually consists of 2 phases:

1) Embryogenesis from EC is composed of small cells with small vacuoles and dense cytoplasm.

2) Development of embryoid bodies. EC is transferred to medium with auxin reduction or removal, reducing nitrogen. Upper culture is sufficient.

Phyto-finch cell culture needs ().

a.Serozoar.

b.Inorganic salts and trace elements.

Carbohydrate sock shaft.

Correct answer: B