If a solution is diluted 5 times with double distilled water, how much double distilled water should

1. PAGE gel electrophoresis buffer configuration 1) Acrylamide monomer storage solution: acrylamide plus n, n'- Alpha bis-acrylamide, stir and dissolve with 40ml of double distilled water until the solution becomes transparent, then dilute to 50ml with double distilled water and filter. Store at 4 °C in a brown bottle for later use.

2) Stacking gel buffer stock solution (Tris-HCl,: dissolved in 40ml of double distilled water and adjusted with 4mol L hydrochloric acid. Then dilute to 50ml with double distilled water.

Store at 4 °C for later use. 3) Separation gel buffer stock solution (Tris-HCl,: dissolved in 80 ml of double distilled water, adjusted with 4mol L hydrochloric acid.

Dilute to 100ml with double distilled water and store at 4°C for later use. 4) 10% (AP) ammonium persulfate: ammonium persulfate dissolved in double distilled water and freshly prepared before use.

5) Electrode buffer (tris, glycine,: plus glycine, dilute to 5 L with double distilled water. Can be stored at room temperature for up to one month.

6) Sample buffer (Tris-HCl, 2 ml of stacking gel buffer stock plus 1 ml of 87% glycerol, bromophenol blue, diluted to 10 ml with double distilled water, can be stored at -20 °C for 6 months. 2. Native-page formula separating glue:

Double distilled water 30% acrylamide solution Tris ( 10% ammonium persulfate solution (W V) 200 L Temed 15 L Stacking gel: Double distilled water 30% acrylamide solution mol ltris ( 10% ammonium persulfate solution (W V) 100 L Temed 10 l 3, native-page electrophoresis Wash the glass plate, rubber pad, and comb with double distilled water, wipe with an alcohol cotton ball, install the electrophoresis tank, and prepare the separating gel (12%) and stacking gel (5%) as shown in Table 1. Ammonium persulfate and temed are added last, and the polymerization begins after addition, which should be mixed immediately and poured between two glass plates.

Pour the separating glue into the two glass plates, should leave a suitable height, so that the front end of the spotting hole is about a distance from the separating glue, slowly add about high double distilled water at the top of the glue, after the polymerization of the separating glue is complete, pour off the upper layer of double distilled water, clean the top layer of the gel with double distilled water, and use absorbent paper to absorb the residual water droplets. Pour the stacking glue into the glass plate interlayer, insert the comb, and after the stacking glue is completely polymerized, remove the comb, and immediately clean the spotting hole with double distilled water. Add electrode buffer, tap the sample into the bottom of the spot well with a microsampler, and electrophoresis at 200 volts.

When bromophenol blue reaches the separating gel, change the voltage to 250 volts and continue electrophoresis until bromophenol blue reaches the bottom of the gel. The gel was peeled off, soaked in 100 ml of substrate solution, stained for 1 hour, and photographed immediately after the tape was colored. The gel is then subjected to regular Coomassie brilliant blue staining.

Search: How much double distilled water should be added to dilute a solution 5 times with double distilled water.

Different substances are taken differently, and double distilled water is generally used, which is low in cost.

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With double distilled water, both plants and animals, double distilled water is used, which is a method of measuring protein content; No other method has been found.

Double distilled water bar, dilution solution in biochemical experiments without using normal saline.

If your experiment requires you to use double distilled water, there are not many ways to improve, the solubility of DNA in pure water itself is not high, and the double distilled water in the laboratory and most of the pure water in the pure water instrument, the pH is acidic, which further reduces the solubility, the feasible way is to find water with weak alkaline pH, which will improve the solubility a little.

If the experiment allows the use of buffer, use TE buffer, which is the most common buffer we use when extracting DNA

Composition: 10 mM Tris-HCl 1 mM EDTA pH = Preparation Amount: 500ml Preparation Method:

Measure the following solutions into a 500ml beaker: 1M Tris-HCl buffer pH = 5 ml EDTA pH = 1 ml Add about 400 ml of dd H2O to the beaker and mix evenly; After the solution is fixed to 500ml, it is sterilized by high temperature and autoclaving; Store at room temperature.

What are primers for? If it is PCR, because the cDNA is relatively stable, it can be diluted directly with double distilled water DDH2O.

The difference between the loading buffers 4 and 5 is that the proportion of the sample is not the same.

4 How to use the sample loading buffer:

1. Use 10 microliters of loading buffer per 30 microliters of protein sample (4-fold dilution). If the protein concentration is too high, it can be diluted with double distilled water.

2. After mixing, heat the 100 water bath for 5-10 minutes to denature the protein.

3. After cooling to room temperature, centrifuge at 10000-14000rpm for 2-5 minutes, and take the supernatant and load the sample directly for electrophoresis.

5 How to use the loading buffer:

1.Dissolve SDS-PAGE loading buffer (5x) at room temperature or in a water bath no larger than 37 °C. Store at room temperature immediately after dissolving in the water bath, and try to avoid leaving it in the water bath for a long time.

2.Mix the protein sample and protein loading buffer (5x) at a ratio of 1 μl of protein loading buffer (5x) per 4 μl of protein sample.

Or heat in a boiling water bath for 3-5 min to fully denature the protein.

4.After cooling to room temperature, the sample can be loaded directly into the SDS-PAGE gel dosing well.

5.Typically, electrophoresis is stopped when the blue dye reaches near the bottom end of the gel.