I can t do a good job of plant virus extraction, so I have experienced experts to help me

The method of plasmid is the method of solution 123.

For DNA, please refer to the method of extracting DNA from animals and plants.

For a faster method, we can refer to our method of colony PCR, which uses a small number of bacteria as a template.

Then the PCR reaction solution is added, and the bacterial cell wall will be lysed off during the PCR high-temperature pre-denaturation, and both DNA and plasmid will be released into the solution as templates to participate in amplification.

If you have a 96-well deep plate (1-2ml) and a platoon gun, it is best to save time and trouble. Toothpicks or pipette heads pick a little bit of bacteria (as long as they are stained with colonies, they do not require large pieces, large pieces are not good), add 500ul culture medium to each well of 96 wells, pick 96 pieces, and then take them to shake the bacteria, culture for 3-5 hours, and the bacteria solution can be directly used as a template for PCR.

As long as the primers are easy to use, they can basically be expanded. We identify positive clones all doing this, you can refer to it.

Hello: Maybe you have the macro feature enabled.

Receive your computer's firewall settings and turn it in.

It should be a problem with the wireless router, I don't know if your wireless router is set up with encryption. This is very important, and can you enter the setup wizard of the wireless router, because if you use it for many years, there will be more or less such problems, and the protection of the route of the organ during thunderstorms is also very important.

It may be a channel issue, and there are other wireless networks that are interfering with your wireless photos. Modify the channel in the router's wireless settings to find a channel with less interference and stable operation.

How is your wireless router configured? Is it directly from the bus? Is it a WAN port or a LAN port? If the WAN port is a static IP or a dynamic IP, is it used? You can't see this here.

In addition, frequent interruptions may be a problem with the wireless routing configuration, or it may be a problem with the telecom operator.

The signal is so good, it is unlikely that there is a problem with the router, see if there is a problem with your computer's wireless network card, and then kill the virus, because viruses can also interrupt the network.

If it is not because the long-distance signal is not good, 1 update the network card driver (if it doesn't work, redo the system).

2. The wireless network card is damaged.

3. The router quality is not good.

If the quality of the home wireless router is not good, there will be such problems.

There is a problem with unlimited routing, change it.

It may also be that the number of frames in the file is too small, or the time is too short, so you can try slow play, very slow, very slow.

There are many antivirus software on the Internet, some domestic and some foreign, and some are free and paid.

I don't know which one you want?

Domestic charges: Kingsoft Drug Tyrant, Ruixing, etc.

Free in China: 360 antivirus.

Foreign charged: Kaspersky (if the computer hardware configuration is low, do not use it, otherwise it will be very stuck.) Free abroad: Little Red Umbrella, McCafe, etc.

Let's redo the system. To format the entire disk, repartition it

Keniu Free Antivirus + Kingsoft Guardian + CP Firewall package your computer is fine.

Rubber compounding is a lead-containing additive, anti-oxidation, anti-aging and the like.

PONA uses a specific column (cross-linked methyl silicone oil and other stationary phases) to separate the detection of trace amounts of benzene in the air at a specified temperature setting, using volatile organic solvents such as methyl silicone oil or low molecular weight.

Let me tell you, these are just like the situation between 360 and Tencent at the beginning, not coordinated. The 360 firewall always reports risks, and it's fine. You can click to continue visiting, and if you have trouble getting it every time, you can add these pages to the trust.

If you have installed anti-virus software, it is good to pay attention to regular anti-virus yourself.

It's obvious that it's been hung up.,Clear the Trojan and the back door.。

I went in and didn't show anything, I'm trying it out.

This has nothing to do with nod32, if you insist on thinking so, then you can uninstall it for comparison, if it has anything to do with it, it may be network monitoring, or bad compatibility with other software.

In fact, you can install a 360 on your own computer.

Reinstall the system in a backup.

In case of poisoning, it's OK to recover from the normal plug-in 360.

Have you installed Thunderbolt yet?

After Thunderbolt is turned on, the data will generally be quietly uploaded in the background; If it is, turn it off.

nod32 blows well, but it's not... It is recommended that you use the Pro version. I've been using it for five years、。。

It shouldn't be that the memory is bad.

It is usually caused by viruses or Trojan horses.

To detect and kill viruses and Trojans, it is really not possible to install a new operating system.

The reason why the motherboard is not compatible with the memory. Try a different memory.

If there is a problem with the computer's system, you can use the 360 first aid kit to fix the system, and that's it.