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Laboratory grade 1 water is mainly used for life science experiments, instrumental analysis experiments.
Modern commonly used experimental instruments include test tubes, beakers, evaporation dishes, etcCrucibles, alcohol lamp, Brinell funnel, gas washing cylinder, drying tube, tray balance, graduated cylinder,Volumetric flasks, burettes, measuring devices, etc. Cleaning, disinfection and sterilization of laboratory instruments is one of the key means to prevent and control infections in the laboratory and ensure laboratory quality.
The handling and disinfection measures for each type of laboratory equipment are different. Strict disinfection and sterilization work is extremely important, which directly affects whether the whole experiment can be carried out smoothly. Therefore, ensuring the health and safety of laboratory instruments has been widely concerned.
Types of laboratory microorganisms: different typesPathogenic microorganismsResistance to disinfectants is different.
Therefore, disinfection must be carried out differently.
1) Bacterial propagules are easily destroyed by disinfectants, generally Gram.
Positive bacteria are more susceptible to disinfectants, while gram-negative bacteria are often more resistant. Procarbs are sensitive to heat, and the disinfection method is mainly thermal disinfection.
2) Bacterial spores have the strongest resistance to disinfection factors.
Among chemical disinfectants, glutaraldehyde, peracetic acid.
It can kill spores, but it is not as reliable as thermal sterilization.
3) The endurance of viruses to disinfection factors varies greatly depending on the type of town, and the endurance of hydrophilic viruses is stronger than that of lipophilic viruses.
4) fungus to dryness, sunlight, ultraviolet rays.
and most chemical drugs have strong endurance.
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resistivity greater than megaohms (25 degrees Celsius); TOC (total organic carbon) less than 1 to 10 ppb, DO (dissolved oxygen) less than 1 to 10 ppb; particle (particle) microns less than 200 liters; Most of the ion content is less than 20 to 50 ppt; Bacterial testing is none. <
Standard for purified water: resistivity greater than megaohms (25 degrees Celsius); TOC (total organic carbon) less than 1 to 10 ppb, DO (dissolved oxygen) less than 1 to 10 ppb; particle (particle) microns less than 200 liters; Most of the ion content is less than 20 to 50 ppt; Bacterial testing is none.
Purified water refers to water that does not contain impurities, referred to as clean water or pure water, which is pure, clean, and does not contain impurities or bacteria, such as organic pollutants, inorganic salts, any additives and various impurities, and is the raw water that meets the sanitary standards of drinking water. It is prepared by electrodialyzer method, ion exchanger method, reverse osmosis method, distillation method and other appropriate processing methods, sealed in a container, and does not contain any additives, colorless and transparent, and can be drunk directly. The best space water and distilled water on the market are pure water.
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There is no grade of pure water, pure water is pure water. There are grades of pure water.
Pure moisture is graded in the following four grades:
1. Distilled water
One of the most commonly used pure water in laboratories is inexpensive, but it is extremely energy-intensive, water-intensive, and slow, and its use gradually decreases. Distilled water removes most of the contaminants in tap water.
2. Deionized water:
Ion exchange resins are used to remove anions and cations from water, but there are still soluble organic matter in the water, which can contaminate the ion exchange column and reduce its efficacy, and deionized water is also easy to cause bacterial reproduction after storage.
3. Reverse osmosis water:
Reverse osmosis water overcomes many shortcomings of distilled water and deionized water, and the use of reverse osmosis technology can effectively remove impurities such as dissolved salts, colloids, bacteria, viruses, bacterial endotoxins and most organic matter in water.
4. Ultrapure water
Ultrapure water is not the same in terms of TOC, bacteria, endotoxin and other indicators, and it should be determined according to the requirements of the experiment, such as cell culture has requirements for bacteria and endotoxin, while HPLC requires low TOC.
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Hello, the consumption of potassium permanganate in drinking purified water (in terms of O2) should not be exceeded. If the consumption of potassium permanganate is high, it is possible that there are microorganisms in the water that exceed the standard, or it may be that some manufacturers increase the amount of disinfectant CLO2 in order to prevent microorganisms from exceeding the standard, thus producing some new organohalogenated compounds, in this case, the general free chlorine will also exceed the standard.
Anions and cations in water can be removed by electrodialysis, reverse osmosis and ion exchange resin technology; The particles in the water can generally be removed by ultrafiltration, membrane filtration and other technologies; At present, the bacteria in water are mostly removed by dosing or ultraviolet lamp irradiation or ozone sterilization in China; The TOC in the water is generally treated with activated carbon and reverse osmosis.
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Laboratory water purification systems typically have the following requirements:
Water quality: Pure water systems need to be able to produce high-purity water to meet the needs of the experiment. In general, laboratory water purification systems should be able to remove impurities such as ions, dissolved solids, organics, and microorganisms from water to meet specific water quality standards.
System Collapse Design: The design of a pure water system should take into account factors such as the water demand of the laboratory, the water quality requirements, and the space constraints of the laboratory. It usually includes components such as water source, pretreatment unit (such as reverse osmosis, ion exchange, activated carbon filtration, etc.), water storage device, disinfection unit, and water delivery system.
Operational stability: The pure water system should have stable and reliable operation characteristics, and be able to continuously supply pure water that meets the requirements for a long time. It should be able to automatically monitor water quality and make the necessary adjustments and maintenance to guarantee the proper functioning of the system.
Cleaning and maintenance: Pure water systems need to be cleaned and maintained regularly to ensure they are working properly and have a long lifespan. The cleaning process should follow the relevant operating procedures, and use appropriate cleaning agents and disinfectants to remove dirt and spine and kill bacteria.
Safety: The pure water system should be safe and reliable to ensure that the operator and the experimental sample are not contaminated with harmful substances. Necessary safety measures should be taken, such as preventing leakage, restricting operating authority, and equipping emergency shutdown devices.
It is important to note that the needs and requirements of different laboratories may vary, so when selecting and designing a pure water system, it needs to be adjusted and configured according to the actual situation. It is advisable to consult and cooperate with a professional supplier or engineer to ensure that the pure water system meets the specific needs of the laboratory.
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The requirements of the laboratory pure water system mainly include the following aspects:
Purity requirements: Laboratory water purification systems need to produce high-purity water to meet experimental and analytical requirements. Water is usually required to have a resistivity of megaohms centimeters (m·cm) or higher, and to remove organic and inorganic contaminants, microorganisms, etc.
Applicability: The laboratory water purification system should be suitable for different types of experimental and analytical needs, such as biochemical experiments, cell culture, molecular biology, etc. It should be able to provide water of different purities, such as ultrapure water, purified water, etc.
Stability: A laboratory water purification system should have consistent performance that consistently produces high-purity lead water and consistent water quality. It should be able to automatically monitor and adjust water quality to adapt to changes in experimental needs.
Easy to operate: The laboratory water purification system should have a simple and easy-to-use operation interface, which is convenient for experimenters to operate and maintain. It should be able to automate water quality monitoring and maintenance tasks, as well as provide alarm and fault diagnosis capabilities.
Safety: The laboratory water purification system should have safety performance to ensure the safety of the experimenter. It should be able to protect the water from external contamination and have safety features such as water leak protection and filter clogging protection.
Economical: Laboratory water purification systems should be economical, taking into account both the purchase and installation costs of the equipment, as well as the operation and maintenance costs. It should be able to use water efficiently and be energy-efficient and environmentally friendly.
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In the national standard GB17323 1998, it is clearly stipulated that the content of chloroform and carbon tetrachloride in drinking purified water shall not exceed milligrams and milliliters respectively. Purified water is the raw water that meets the sanitary standards for drinking water. It is made by electrodialyzer method, ion exchanger method, reverse osmosis method, distillation method and other appropriate processing methods, sealed in a container, and does not contain any additives and hail tung, colorless and transparent, and can be directly drunk.
Strengthen their own health management, strengthen the awareness of food hygiene and quality, designate a leader to be responsible for health work, set up a full-time health inspection agency, strengthen the testing of water sources, packaging, air and products in the filling room, formulate a health management system from water source management, sterilization, filling, packaging to personal hygiene, and designate a special person to supervise the implementation, strengthen the training and learning of food hygiene knowledge, focus on mastering disinfection methods and clarify the key links of microbial easy contamination.
According to the characteristics of the water source, the production process should be reasonably and scientifically designed, equipped with necessary water treatment and production equipment, and the sterilization system in line with water disinfection should be selected. China's mineral water and purified water mostly use ultraviolet light, ultrafiltration and ozone as sterilization and disinfection facilities. However, years of experience have proved that the poor reliability of the first two is the main factor causing product failure, and the use of ozone sterilization is considered to be the best method at present.
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In the national standard GB17323-1998, it is clearly stipulated that the content of chloroform and carbon tetrachloride in drinking purified water shall not exceed milligrams and milliliters respectively. Purified water is the raw water that meets the sanitary standards for drinking water. It is prepared by electrodialyzer trapping method, field racket exchanger method, reverse osmosis method, distillation method and other appropriate processing methods, sealed in a container, and does not contain any additives.
In the national standard GB17323-1998, it is clearly stipulated that the content of chloroform and carbon tetrachloride in drinking purified water shall not exceed milligrams and milliliters respectively. Purified water is the raw water that meets the sanitary standards for drinking water. It is prepared by electrodialyzer method, ion exchanger method, reverse osmosis method, distillation method and other appropriate processing methods, sealed in a container, and does not contain any additives, colorless and transparent, and can be drunk directly.
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