How do you write a review of plant tissue culture?

I don't know much about plant tissue culture, so I'm going to write a rough framework if I'm going to start with it.

Title: Research status of plant tissue culture technology.

Abstract:This paper summarizes the research status of plant tissue culture at home and abroad, the current development or future development, and the significance. A high-level summary of what you have stated in the review, what you are trying to illustrate, and what role it can play.

Probably more than 50 words, not too many.

Body: (including preface, main body, and summary).

Foreword: The concept, meaning, current development or existing problems of plant tissue culture, leading to the purpose of your writing.

Main body: The current situation of plant tissue culture technology at home and abroad can be described horizontally, that is, what technologies are available, by whom, specific means, and what are the results. Give your own opinion in the comparison.

It can also be written longitudinally according to the research and development time of plant tissue culture. For example: Phase 1 development: main researchers, what results have been achieved. Until now, it is possible to propose a better direction for future development.

Plant tissue cultureThere are three ways to disturb the omen: guess the celebration

1. Embryo culture.

Mature or immature embryos isolated from ovules are aseptically cultured ex vivo for explants.

2. Organ culture.

In vitro aseptic culture of plant roots, stems, leaves, flowers, fruits and other organs as explants, such as the root tips and cut segments of roots, stem tips, stem nodes and cut segments of stems, leaf primordiums, leaves, petioles, leaf sheaths and cotyledons of leaves.

Petals, stamens (anthers) of flower vessels.

filigree), ovules, ovary.

In vitro aseptic culture of fruits, etc.

3. Cell culture.

In the form of a single free cell (e.g., with AHAs.

Somatic cells isolated from tissues by enzymes.

or pollen cells, egg cells.

It is an ex vivo sterile culture of inoculums.

Plant tissue culture significance:

1. Tissue culture is an important means to study the law of plant growth and differentiation, and tissue culture is a technology for cultivating explant regeneration organs or plants under artificial control conditions, which can study the growth and differentiation of the cultured part without interference from other parts of the plant body, and can use various culture conditions to affect their development process.

2. Tissue culture is the basic technology for carrying out bioengineering, and various gene transfer and gene recombination technologies are established on the basis of tissue culture.

3. Tissue culture can rapidly propagate plant seedlings, and at present, tissue culture has been widely used in the rapid propagation of clones, the cultivation of virus-free seedlings, the breeding of new varieties, the preservation of artificial seeds and germplasm, and the industrial production of medicinal plants and secondary biomass.

Rapid propagation of some rare plants or plants with greater economic value, the preservation of detoxified plant seed remedial resources, the rescue of endangered plants, the acquisition of haploid plants through anther and pollen culture, and the application of shortening the breeding period and embryo culture.

Cell fusion. Applications for cultured cell mutants.

It is used in genetics, molecular biology, cell biology, histology, embryology, genetic engineering, bioengineering, etc.

Tissue culture materials are used as plant bioreactors.

For research in other unknown sciences.

In a broad sense) is also known as in vitro culture, which refers to the technology of isolating tissues, organs or cells that meet the needs of plants, protoplasts, etc., through aseptic operation, and cultivating them under artificially controlled conditions to obtain regenerated complete plants or produce other products with economic value. Plant tissue culture concept.

In the narrow sense) refers to the cultivation of various parts of plant tissues, such as cambium, parenchyma, mesophyll tissue, endosperm, etc., to obtain regenerated plants, and also refers to the culture of calli produced from various organs in the culture process, and the callus is then redifferentiated to form regenerated plants.

Classification of plant tissue culture:

1. Embryo culture of embryo culture plants, including embryo culture, endosperm culture, ovule and ovary culture, as well as embryo culture technology for in vitro fertilization.

2. Organ and tissue cultureOrgan culture refers to the cultivation of all or hungry parts of a certain organ or organ primordium of a plant, including stem segment closure, shoot tip, tuber, bulb, leaf, inflorescence, petal, ovary, anther, receptacle, fruit, seed, etc. Tissue culture is broadly and narrowly defined. Generalized:

Cultivation of various types of explants is included. Narrow sense: including cambium tissue, meristem, epidermal tissue, parenchyma tissue and various organ tissues, as well as calli produced by their culture.

3. Cell cultureCell culture includes a large number of culture systems carried out by bioreactors to promote cell growth and biosynthesis, and single-cell culture that uses single-cell cloning technology to promote cell growth, differentiation and the formation of complete plants.

4. Protoplasts cultured plant protoplasts are bare cells that are wrapped by the plasma membrane and have vitality that have been removed from the cell wall.

Categories: Science & Engineering.

Problem description: What materials are easier for novices to choose for plant tissue culture, and please write down the specific plan and precautions, thank you!

If you have a satisfactory answer, add 20 points, thank you!

1. Collection of culture materials.

Tissue culture uses a wide range of materials, from roots, stems, leaves, flowers, buds, and seed cotyledons, to pollen grains and anthers.

2. Disinfection of culture materials.

1) Rinse the material with distilled water first, then blot the moisture on the material with sterile gauze or absorbent paper, and cut it into small pieces with a sterilizing blade.

2) Soak the material in a 70 sprinkle for 30 60 seconds in a sterile environment.

3) Then transfer the material into the saturated solution of bleaching powder or mercury water for 10 minutes to disinfect.

4) Rinse with sterile water after removal.

Three or four times. 3. Preparation of explants.

The sterilized materials are cut into centimeter-thick pieces in a sterile environment with sterile knives, scissors, tweezers, etc., which is the explant. It is strictly forbidden to touch the material with your hands during operation.

4. Inoculation and culture.

In a sterile environment, the cut explants are immediately attached to the medium. After inoculation, bottles and tubes are sealed with sterile cotton wool or caps, and petri dishes are sealed with sterile tape. The medium should mostly be kept at around 25.

In order to expand the reproduction coefficient after the formation of new shoots, the material was divided or cut into the proliferation medium to facilitate the improvement of the proliferation rate.

Adventitious buds and lateral shoots formed by the above culture generally do not have roots, and a strong root system can be obtained after 1 month after being transferred to the rooting medium.

Fifth, the transplanting of tissue culture seedlings.

The test-tube seedlings enter the natural environment from sterile to a stable environment of light, temperature and humidity, and the seedlings must be quietly refined. Generally, before transplantation, the culture container is opened, placed in natural light indoors for 3 days, and then the seedlings are taken out, and the nutrient base on the root system is rinsed with tap water, and then planted into the prepared substrate, which is best disinfected before use. Before transplanting, it should be properly shaded and kept high air humidity and too humid.

Tissue culture using solid media.

Ingredients contained in solid medium: 1. Inorganic nutrients include N, P, K, CA, MG, S and trace elements Fe, B, Mn, Cu, Zn, Mo, Cl, I, etc.;

2. Organic nutrients include sugars, amino acids and their amides, which provide carbon, hydrogen, oxygen, and nitrogen for plants;

3. Physiologically active substances include vitamins, niacin, inositol, single nucleotides and their bases.

role="Figure the process of tissue culture seedlings.

1) It can maintain the inherent traits and characteristics of the original variety.

2) Save propagation material. Collecting a small fraction of the vegetative organs can produce a large number of flower seedlings.

3) Fast reproduction. A small piece of tissue from a good mother plant can be multiplied in a year after in vitro culture under suitable conditions.

4) Save land. Tissue culture materials are placed in triangular flasks or test tubes, usually a 30-square-meter culture room can place more than 10,000 triangular flasks, can propagate tens of thousands of seedlings. Moreover, it has a fast turnaround and can be continuously cultivated throughout the year.

5) Go to the virus. At present, many flower virus diseases are more serious, which directly affects ornamentation and export. Detoxification can be carried out through tissue culture, making it a non-toxic seedling.

6) Rejuvenated varieties. For flower varieties that have been vegetatively propagated for a long time and have begun to degenerate, the use of tissue culture method to propagation can transform ontogeny into a young stage.

7) Asexual reproduction is possible. For some flowers and trees that are difficult to reproduce asexually in production, or flowers and trees that cannot be propagated asexually, a large number of vegetative seedlings can be obtained in a short period of time under the special conditions of tissue culture.