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The mycelium of edible fungi reproduces quickly and is easy to mutate, and it is difficult to preserve the strains. Here are 4 methods that are more effective and easy to implement.
1. Liquid paraffin preservation method After the liquid paraffin is autoclaved, put it in an incubator for a few hours, or put it in a desiccator for several days, use a sterile pipette or syringe to aspirate the liquid paraffin, and inject it into the bacterial test tube. The injection volume should be about 1 cm higher than the bevel tip. Plug the tampon, seal it with wax, and store it upright at a low temperature for 5 years.
2. Bran preservation method Weigh a certain amount of bran, add water or nutrient solution, mix well and use. The wet bran is loaded into a test tube, autoclaved and then inserted into the culture, and placed at an appropriate temperature to culture. Once the mycelium is well developed, place the tubes at room temperature for drying.
After drying, store it in a dry place below 20 and can be stored for 3 to 5 years.
3. Normal saline preservation method Insert the strain into the potato culture medium, use a 250 ml triangle bottle to fill the 60 ml culture medium, shake the culture or shake 5 10 times a day, culture for 5 7 days, and then move the formed mycelial balls into a test tube filled with 5 ml of sterile normal saline, move 4 5 mycelial balls into each tube, plug cotton eggs, and seal the nozzle with wax. It can be stored for 1 year at low temperature.
Fourth, wheat grain preservation method take high-quality wheat, soak it in 20 water for 5 hours after washing, dry it slightly, and load it. The loading amount should be 1 4 1 3 of the pipe length. Sterilize, shake while hot, cool and insert mycelium.
Incubate at a moderate temperature and terminate when sparse hyphae appear on most of the grains. Then, place it in a dry place below 25 and can be stored for 1 2 years.
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The mycelium of edible fungi reproduces quickly and is easy to mutate, and it is difficult to preserve the strains. In short, no.
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Centrifugal management should not exist, biochemical management should exist.
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Let's use a round bottom.
The effect of a round bottom is not much different from that of a pointed bottom.
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EDTA, sodium citrate, and heparin are all plasma after anticoagulation and centrifugation, and the fluid exuded after blood clotting is serum.
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Anything that is added with anticoagulants is plasma. Because the blood in vitro fibrin will automatically coagulate and precipitate, and finally obtain serum. Anticoagulants are added to obtain plasma.
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Regardless of whether EDTA or sodium citrate or heparin, as long as the supernatant centrifuged after the addition of anticoagulant is plasma, the supernatant centrifuged without anticoagulant is serum.
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Regardless of the anticoagulation, all that is obtained after centrifugation is plasma.
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Heparin is anticoagulated and centrifuged after plasma, and the rest is unknown.
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I'm sorry, I don't know about that.
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Centrifugation allows DNA to be separated from proteins so that it can be examined and nuclei are precipitated
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Reduce the error of other components in the blood on the test.
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Hello! There is no rule from the railway authorities that you can't bring serum specimens, so you can take them on the train! But please keep your specimens safe so as not to annoy other passengers or yourself! Have a great trip!
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OK. As long as it is not a strong acid, a strong alkali and other corrosive liquids are fine.
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No problem! It's no problem to go through the security check!
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Here's a literature, don't anticoagulate.
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c Because of the presence of clotting factors in the blood, fresh blood will agglutinate after 24 hours without anticoagulants and sink to the bottom of the tube.
After the blood clotted in this isolated body, the fluid released by the blood clot is released by the condensation of the blood clot, known as serum.