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Genetic engineering, also known as gene splicing technology and DNA recombination technology, refers to the theoretical basis of molecular genetics, with the modern methods of molecular biology and microbiology as a means, different genes according to the pre-designed blueprint, in vitro construction of hybrid DNA molecules, and then into living cells to change the original genetic characteristics of organisms, obtain new varieties, and produce new products.
Genetic engineering involves cross-species and asexual amplification of exogenous genes into the cells of another different organism for propagation, and exogenous DNA can be amplified in large quantities and expressed at high levels in host cells.
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1. Universality of codons: Whether it is hail prokaryotic cells or eukaryotic cells, the genetic code they use is the same, that is, every 3 adjacent bases determine an amino acid, this rule is generally applicable between prokaryotic cells and eukaryotic cells. It is for ribosomes to code for proteins in the cytoplasm, and in the microchondria, the DNA contained in the mitochondria itself can also code for proteins, and the rules of the codons used may be different from those in the cytoplasm.
2. Basis of molecular genetics: Molecular genetics is a sub-discipline of genetics that studies the mechanism of biological inheritance and variation at the molecular level. The research topic of classical genetics is mainly the transmission of genes between parents and offspring. Molecular genetics mainly studies the nature of genes, the function of genes, and the variation of genes.
The early research of molecular genetics used microorganisms as materials, and its formation and development are closely related to microbial genetics and biochemistry.
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Hello dear, genetic engineering is a kind of genetic technology that uses modern methods such as molecular genetics, molecular biology and microbiology to construct hybrid DNA molecules in vitro according to the blueprint of different genes in vitro, and then introduce them into living cells to change the original genetic characteristics of organisms, obtain new varieties and produce new products. Embedded genetic engineering can break the boundaries of species, change the heritability of organisms at the genetic level, and provide useful products and services for humans through engineering.
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Genetic engineering is a major in bioengineering, also known as biotechnology or biotechnology. It is a specialty that applies the principles of biology and engineering to form new biological varieties with specific traits for the unique functions of biological materials and organisms. This major cultivates interdisciplinary talents with basic knowledge of biology and engineering, as well as basic knowledge of natural sciences and humanities, and can be engaged in the production of bioengineering products, process design, production management, new technology research and new product development in biotechnology and engineering and other related fields.
Material Supplement: Core courses of genetic engineering: cell biology, biochemistry, molecular biology, immunology, microbiology, genetics, bioinformatics, genetic engineering, cell engineering, biopharmaceutical, microbial technology, fermentation engineering, ecology, chemical engineering principles and chemical engineering technology.
I wish you a happy life and success in your studies, and I hope mine will be helpful to you.
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Genetic engineering (GeneticEngineering), also known as gene splicing technology and DNA recombination technology, is based on molecular genetics as the theoretical basis, with the modern methods of molecular biology and microbiology as a means, according to the pre-designed blueprint of different genes, in vitro to construct hybrid DNA molecules, and then introduce into living cells to change the original genetic characteristics of organisms, obtain new varieties, and produce new products. Genetic engineering technology provides a powerful means for the study of gene structure and function.
Genetic engineering, also known as gene splicing technology and DNA recombination technology, refers to the theoretical basis of molecular genetics, with the modern methods of molecular biology and microbiology as a means, different genes according to the pre-designed blueprint, in vitro construction of hybrid DNA molecules, and then into living cells to change the original genetic characteristics of organisms, obtain new varieties, and produce new products. >>>More
a.A marker gene is a gene with a known function or a known sequence that can play a specific role as a marker. In the sense of genetic engineering, it is an important marker for recombinant DNA vectors, which is usually used to test the success of transformation. In the sense of gene mapping, it is a tool for marking the gene of interest, which is usually used to detect the localization of the gene of interest in cells. >>>More
4.Using mRNA as a template, catalyzed by reverse transcriptase, reverse transcribed into cDNA in vitro, and ligated with an appropriate vector (commonly used phage or plasmid vector) to transform the recipient bacteria, each bacterium contains a piece of cDNA and can multiply and expand, so that the collection of cDNA clones containing all the mRNA information of the cell is called the cDNA library of the tissue cell. >>>More
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