What is reconditional pcr, does anyone know the details

Repair plan change (inspection) request.

Let's watch "Molecular Clone" for this thing! To do RT-PCR, it is necessary to pay attention to the housekeeping gene control, and real-time PCR should also pay attention to the internal and external standards!

The specificity of the PCR reaction is determined by:

Primers bind correctly to template DNA specifically;

the principle of base pairing;

Faithfulness of the Taq DNA polymerase synthesis reaction;

Specificity and conservation of target genes.

The correct combination of primers and template is key. The binding of primers to the template and the extension of the primer strand follow the principle of base pairing. The fidelity of the polymerase synthesis reaction and the high temperature resistance of Taq DNA polymerase allow the binding (refolding) of the template to primers in the reaction to be carried out at a higher temperature, the specificity of binding is greatly increased, and the amplified target gene fragment can be maintained with high accuracy.

The degree of specificity is higher by selecting the target gene region with high specificity and conservation.

Be careful with the tip and change the tip to avoid cross-contamination.

Operation on ice. Wear a mask and don't speak.

Fundamentals of PCR technology: This technology relies on the enzymatic synthesis of DNA polymerase in the presence of template DNA, primers, and four deoxyribonucleotides. DNA polymerases use single-stranded DNA as a template to initiate synthesis with the help of a small piece of double-stranded DNA, which is partially double-stranded by combining one or two synthetic oligonucleotide primers with a complementary sequence in the single-stranded DNA template.

At the right temperature and environment, DNA polymerase adds deoxymononucleotide to primer 3

The DNA amplification process is divided into the following basic processes, and different processes require different temperatures such as 90-95° denaturation to unwind the DNA.

Annealing and cooling at 55-60° to allow the unwinded single strands to bind to the primers respectively and extend Heating at 70-75° Starting from the primers for a complementary cycle Repeat the above process to amplify the gene of interest in large quantities.

PCR for DNA replication and you're asking about the SPY-PCR check, which is to check the sex, only the Y chromosome has SPY on it

It means "20 yuan will be charged for every time you do PCR", what kind of test is it for Brother Bu?

If it is sequencing, it is a successful sequencing of 20 pieces of base dust slag at a time, and if it is not successful, no money will be charged. Quietly.

The QX100 droplet digital PCR system is the third generation of PCR. Droplet digital PCR is a unique digital PCR technique of Bole Life. DDPCR enables absolute quantification of DNA or RNA molecules with unmatched accuracy, eliminating the need for standard curves.

Micro-leasing or droplet digital PCR greatly improves the scalability and practicability of digital PCR.

Look at how many PCR reaction tubes you put in 15 pairs of primers, if there are 15 times for 1 sample as mentioned above, then 10 samples should be 150 times, using 1/4 of the kit, ** about 200 yuan.