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Leica Microsystems is a global pioneer in the field of microscopes and scientific instruments. The company is headquartered in Wetzlar, Germany. From its humble beginnings in the 19th century as a family business, the company has become a world-renowned company with an unrivalled spirit of innovation.
Close cooperation with the scientific community is the key to Leica Microsystems' tradition of innovation, and it is the corporate goal of Leica Microsystems to put the user's ideas into practice and tailor the solution to the user's needs. Leica Microsystems is represented in more than 100 countries, has sales and service organizations in 19 countries, and has a global network of leading companies.
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There is no better microscope, only which one is more suitable for your needs, and the most important thing to choose a microscope is the following two points:
1. What effect do you want and how many times do you magnify it?
Eukaryotic cells: 10-100 m; Prokaryotic cells: 1-10 m; Nucleus:
Approximately 7cm; Mitochondria:; Most viruses: 20-100 nm.
Optical microscopes can distinguish at a minimum of 200 nm; Smaller mycoplasma and viruses need to be observed using an electron microscope. Total optical magnification = magnification of eyepiece x objective magnification, digital total magnification = eyepiece magnification x objective x digital magnification. Therefore, it is important to choose the appropriate microscope and configuration according to the size of the sample being observed.
2. The quality of the optical system.
Resolving power is the ability to observe the details of a sample and the speed at which an image can be resolved – another important factor to consider. Objective lenses are crucial in optical systems, and the higher the numerical aperture (NA) of the objective, the greater the resolution. The level of detail and budget that the user needs to see to choose the objective with the appropriate numerical aperture (NA).
In addition to the numerical aperture (NA), the degree to which the microscope objective corrects for spherical and chromatic aberration also needs to be evaluated. Choose the best objective within your budget. The quality of the objective lens will directly affect the quality of the data, so this is a very important factor to consider.
There are many types of Leica microscopes, Leica biological microscopes, Leica stereo microscopes, etc., which can also be classified according to different uses, the structure of the instrument, the magnification method and the relationship between the light specimen. It can generally be divided into two categories: optical microscopes and non-optical microscopes (electron microscopes). Optical microscopes are divided into simple microscopes (elementary) and compound microscopes (intermediate and advanced) according to the simple and traditional structures, and users can choose according to their own needs.
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There are many Leica industrial microscope products, different products have different advantages, and the following aspects should be considered when choosing an industrial microscope
1. All-round free observation.
Samples with uneven surfaces can be quickly obtained with a 3D image that can be freely adjusted in size, orientation, ruler, and color.
dContour measurement.
Obtain height information through contour lines, easily draw lines, and measure 12 kinds of 3D data such as height, angle, and R angle with one click.
3. Volume measurement.
By moving the datum, the volume height information of the intercepted part is measured, and the volume value is quickly obtained.
An industrial microscope is a symbol of mankind's entry into the atomic age, an optical instrument composed of one or more lenses, an instrument that magnifies tiny objects into the human eye. The first thing to say about industrial microscopes is stereo microscopes, which are mainly used for on-site inspection, PCB, liquid crystal and other industries. Secondly, the metallographic microscope meets the requirements of material analysis, etc., and the steel and gold processing industries require measuring microscopes, which are more used in microelectronics and fine processing industries.
Leica Microsystems has a rich cultural heritage and a long tradition, and is home to several well-known brands, including Leitz, Wild, Cambridge Instruments, Reichert-Jung and American Optoical. Leica industrial microscopes are widely used in automotive manufacturing, steel, metallurgy, electronics, petrochemical, food and pharmaceutical industries. Leica Microsystems helps users identify, document and report efficiently down to the smallest detail.
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The choice of optical microscope depends on the needs of the user, and there are many classification methods for optical microscopes, which can be divided into trinocular, binocular and monocular microscopes according to the number of eyepieces used; According to whether the image has a three-dimensional sense, it can be divided into stereo vision and non-stereo vision microscope; According to the object of observation, it can be divided into biological and metallographic microscopes.
According to the optical principle, it can be divided into polarization, phase contrast and differential interference contrast microscopy; According to the type of light source, it can be divided into ordinary light, fluorescence, infrared light and laser microscope. According to the type of receiver, it can be divided into visual, photographic, and television microscopes, etc. Commonly used microscopes include binocular continuous zoom stereo microscope, metallurgical microscope, polarizing microscope, ultraviolet fluorescence microscope, etc.
Users can choose according to their needs, and the Leica optical microscope is still good, with clear images and a variety of products, such as the Leica DM2700 M, which can be used for a variety of routine inspection tasks – metallography, geoscience, forensic investigations, materials quality control and research.
When using an optical microscope, you must pay attention to maintenance, and use a balloon to remove the dust on the surface; stains, which can only be cleaned with pure solvents, distilled water, and special tools; Do not use paper, fiber cloth, etc. to wipe it, as it is easy to cause damage to the objective lens, so do not be careless. When there is fog and mildew on the surface of the optical components, it must be professionally maintained by professional engineers.
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The sales volume of Leica ophthalmic microscopes is still quite large. When choosing a microscope, you should pay attention to the following properties:
1) The eyepiece images are coincident and consistent.
The images in each eyepiece of the microscope should coincide. Among them, the main one is a binocular solid microscope, and the images seen by the two eyes should have a good coincidence of coexistence.
2) Illumination and clarity.
The illumination should be well and uniformly focused, and the field of view of the illumination area and the microscope should coincide. The physical properties of the light source should be adapted to the needs of use, and the light source should not damage the wound during a long period of use. Coaxial lighting should be available.
There are two types of microscope illumination: Internal light source - the light source is combined in the microscope and the direction of irradiation is coaxial with the microscope, which is suitable for the illumination of deep tissues. External light source – The light source is located outside the microscope and is divided into two types of coaxial and different axis illumination systems.
3) Moderate depth of field range.
The microscope requires that the shallow or deep parts in use can be clearly observed, and the three-dimensional sense is strong. Operate in a small shade range and do not need to adjust the focal length. Therefore, there should be a certain range of depth of field requirements for the microscope.
The primary mirror is 45° to the microscope body, while the assistant mirror is 30° to the microscope body. When adjusting the focal length of the microscope, the center of the eyepiece field should be clear.
Leica Microscopes are designed to improve user comfort through the seamless integration of high-end imaging technology, with OCT and high-resolution 3D or 2D display and recording, and a full range of modular and upgradable observation accessories are available to meet the user's individual needs and ensure a comfortable working posture.
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When choosing a digital microscope, it is important to note that the digital microscope can produce an upright three-dimensional image of space when observing an object. A good microscope is a good microscope with a strong sense of stereoscopism, clear and wide imaging, and a long working distance.
The digital microscope is easy to operate, intuitive, and has high verification efficiency, and can enlarge the image of the physical object and display it on the computer screen, which can be saved, enlarged, printed, and equipped with measurement software to measure various data.
The main advantage of digital microscopes is that traditional optical microscopes can only be used by one person, it is difficult to share microscope images, and special instruments are often required to capture images within the microscope.
The digital microscope is made of a combination of photographic devices and software, which can meet various applications from daily inspection to complex analysis.
The Leica Digital Microscope is a good product, and some of Leica's digital microscopes have an tilt function that allows you to view the sample from different angles. With the imaging function, users can dynamically record the view of the sample during tilt or rotation for sharing and analysis of observations.
1. Take the mirror and place it.
1 Hold the arm with your right hand and hold the base with your left hand. >>>More
In fact, ordinary optical microscopes are based on the imaging principle of convex lenses, and they need to go through two imaging of convex lenses. The first time is imaged through the objective lens (convex lens 1), the object should be between one and two times the focal length of the objective lens (convex lens 1), and according to the principles of physics, the real image should be magnified and inverted. Then, the first image of the object is used as the "object", and the second image is taken through the eyepiece. >>>More
To say which brand is good, we must first clarify the selection criteria of the metallographic microscope, and consider the continuous stability of the mechanical properties of the metallographic microscope in use, which we call the continuous stability of mechanical properties. Metallurgical microscope is a high-precision optical instrument, its service life can reach more than 30 years, users should also investigate the manufacturer in the manufacture of materials, manufacturing accuracy, mechanical design of the scientific and reasonable. >>>More
Optical microscope: The objective lens is located near the object being observed and is the lens that achieves the first level of magnification. Several objectives with different magnifications are mounted on the nosepiece at the same time, and the rotation of the converter allows the objectives of different magnifications to enter the working optical path, and the magnification of the objective lens is usually 5 100x. >>>More
Precautions for use of the microscope and its use.