Agilent 1100 LC performance indicators

It is recommended that you go to the "Biochemical Chromatography Network" to have a look.

The Agilent LC 1220 outputs a series of data after an analytical experiment. This data can often be processed and analyzed by the software that comes with the chromatograph. Here are some common data types and how to handle them:

1.Chromatogram: Chromatogram is one of the most common outputs in liquid chromatography experiments.

It shows an image of the peak shape of the different components in the sample, typically including information such as peak width, peak height, retention time, relative peak area, etc. You can use the software that comes with your chromatograph for peak identification, peak area integration, peak area comparison, and more. 2.

Mass spectra: If your liquid chromatograph is equipped with a mass detector, you can get the corresponding mass spectral output. Mass spectra usually show the mass peaks of different molecules in a sample, and you can use the mass spectrum to identify the molecular structure of the sample, quantify it, and more.

3.Data report: Some liquid chromatograph software can generate data reports, including sample name, detection conditions, analysis results, and other information.

You can save the data report as a text file or PDF file for later reference and analysis. It is important to note that liquid chromatography data processing requires a certain amount of professional knowledge and experience, and it is recommended that you understand the operation method of the chromatograph and the related analysis techniques before performing the analysis. If you encounter problems with data processing, you can consult the relevant professional or the technical support department of the chromatograph manufacturer.

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Check the detection range of the liquid chromatograph to make sure it can detect absorbance at 280 nm. 2.Check the detection spectrum of the liquid chromatograph to make sure it can detect the absorbance at 280 nm.

3.Check the detection sensitivity of the liquid chromatograph to ensure that it can detect absorbance at 280 nm. 4.

Check the detection accuracy of the liquid chromatograph to ensure that it can detect absorbance at 280 nm. 5.Check the detection stability of the liquid chromatograph to ensure that it can detect absorbance at 280 nm.

Personal tip: When using a liquid chromatograph, you should first check the detection range, spectrum, sensitivity, accuracy, and stability of the liquid chromatograph to ensure that it can correctly detect the absorbance at 280nm. In addition, the performance of the liquid chromatograph should be checked regularly to ensure that it is able to correctly detect the absorbance at 280 nm.

Performance indicators of the detector include: sensitivity, detection limit.

Linear range, response speed, stability, selectivity.

Sensitivity: Refers to the amount of substance that passes through the detector.

The rate of change in the response value of the substance when it changes.

Detection limit: unit volume when generating 2 times the noise signal.

The amount of carrier gas that enters the detector per unit of time. Note that it is currently more accepted to be 3 times. Sensitivity and detection limits are indicators of how sensitive a detector is to a substance from two different perspectives. The higher the sensitivity and the lower the detection limit, the better the detector performance.

Linear Range: Refers to the range in which the response signal is linearly related to the injection quality or concentration of the component being measured. It is usually expressed as the ratio of the maximum allowable injection volume to the minimum detected volume.

The larger the ratio, the wider the linear range of the detector, indicating that the detector can accurately determine a large number of components or trace components in the sample. The linear range of different types of detectors also varies greatly. For example, the linear range of hydrogen flame detectors can reach 107, and thermal conductivity detectors.

It's around 104.

The other points are very well understood.

Series Agilent 1100 Pump System.

Electronic Flow Control Valve (EFC) controlled capillary liquid phase pump system, high precision, wide flow rate range Column flow rate range: 1-20ul min; 10-100ul min (optional).

closed) high-pressure preparative pump systems, unit or dual high-pressure preparative pumps.

Flow Rate Range: Analytical Pump System.

Flow Rate Range: Unit Pump:

Binary Pump: Quaternary Pump:

A complete range of Agilent 1100 series injection systems.

Standard manual sampler (analytical or preparative).

Standard autosampler.

Vial capacity: up to 100 (2mlx100) Injection volume: Optional.

Microdisc autosampler.

Vial volume: 2x96 (plate), 2x386 (plate), or 100x2ml injection volume: standard).

Optional. Micro Standard Autosampler Microdisc autosampler.

Injection volume: standard); Optional).

Thermostatic standard autosampler Microdisc autosampler.

Temperature range: 4-40 steps can be set in 1 step

Model 220 Microplate Autosampler Combined Chemical Sample Management System.

Vial capacity: tubes of various sizes Up to 12 microplates (96-well plates, 384-well plates) Injection volume:

Agilent 1100 series detectors.

Variable wavelength scanning ultraviolet detector (VWD).

Wavelength range: 190 600nm

Multi-wavelength detector (MWD).

Wavelength range: 190 950nm (dual light source).

Diode Array Detector (DAD).

Wavelength range: 190 950nm (dual light source).

Fluorescence detector (FID).

Excitation wavelength: 200-700nm;

Emission wavelength: 280-900nm

Spectral storage: full spectrum.

Refractive index detector (RID).

Temperature control: room temperature +5 to 55

Built-in automatic purge valve and automatic solvent circulation valve.

Electrochemical detector (ECD).

LC MS Quadrupole Mass Detector (MSD).

LC MS Ion Trap Mass Detector (Trap MSD), column heater, and degasser assembly.

Column heater. Temperature range: 10 to 80 degrees at room temperature

Optional: Column changeover valve.

Fast and high-separation liquid phase system.

Binary liquid chromatography Quaternary liquid chromatography system.

Unit liquid chromatography.

Purification system. HPLC-Chip MS System.

Nanoflow liquid chromatography system.

Capillary liquid chromatography system.

1200 Series Detectors.

Diode array detector sl

Diode array detectors.

Fluorescence column detectors.

Multi-wavelength detector.

Multi-wavelength detector SL

Refractive index detector.

Variable wavelength detector.

Multi-wavelength detector SL

Regulatory adaptability to meet the most stringent regulatory requirements.

There is a wide range of more than 70 RRHT columns with a wide range of applicability.

Convenient isogradient or gradient analysis with four solvents for rapid method development and rapid setup of mobile phases, as well as flushing HPLC systems.

Wide flow range with flow rates up to 10 ml min and dwell volumes from 00-1100 l for microbore, standard, and semi-preparative applications.

Programming and control is easy with the Instant Pilot component or with Agilent chemistry workstations or ezchrom software.

Includes a micro-vacuum degasser for high degassing efficiency, hassle-free operation and maximum performance, without the need for helium at all.

Quick replacement of maintenance parts directly from the front panel.

Quickly diagnose issues with self-diagnostics, built-in logs, and pre-programmed test methods.

Early Maintenance Feedback (EMF) continuously monitors instrument usage and user-preset limits over a long period of time, providing feedback if the limits are exceeded.

Upgradable and expandable across all Agilent 1200 Series HPLC component ranges.

The new generation of Agilent 1200 liquid chromatography system is carefully built to achieve the perfect combination of chromatographic resolution, analysis speed, detection sensitivity and sample throughput. The modular design makes it easier for users to get the ideal configuration for their application needs.