What to pay attention to when using a microscope, what to pay attention to when using a microscope

Precautions for microscope use.

Precautions for microscope use.

1 When moving the microscope, hold the lens arm with one hand, hold the lens holder with the other, and place both upper arms close to the chest wall. Do not hold one hand diagonally, swinging back and forth to prevent the lens or other parts from falling.

2 When observing specimens, the microscope should be kept at a certain distance (5cm) from the edge of the laboratory bench to prevent the microscope from tipping over and falling to the ground. The angle of inclination between the mirror column and the mirror arm should not exceed 45 degrees, and it should be restored immediately after use.

3. When using, you should strictly follow the steps, be familiar with the performance of each part of the microscope, and grasp the relationship between the rotation direction of the coarse and fine adjustment buttons and the lifting of the lens barrel. When turning the thick knob downward, the eye must be fixed on the object lens.

4 When observing temporary specimens with liquid, cover sheets should be applied, and tilted joints should not be used to avoid liquid contamination of the lens and microscope.

5. The thick and fine adjustment knobs should be used together, the fine adjustment knobs should not be rotated excessively in one direction, and when adjusting the focal length, the lens barrel should be lowered from the side to avoid crushing the specimen and lens.

6. When observing specimens with a monocular, both eyes should be opened at the same time, the left eye should be used to observe the image, the right eye should be used for drawing, the left hand should adjust the focus, and the right hand should move the specimen or drawing.

7. It is forbidden to unscrew or change parts such as eyepieces, objective lenses, and condensers.

8 If there is dirt on the optical parts of the microscope, you can wipe it off with paper or silk cloth, and do not wipe it with fingers, coarse paper or handkerchief to prevent damage to the mirror.

9. All corrosive and volatile chemical reagents and chemicals, such as iodine, ethanol solution, acids, alkalis, etc., should not be in contact with the microscope, and should be wiped clean immediately if accidentally contaminated. Do not remove the eyepiece arbitrarily and beware of dust falling into the lens barrel.

10 After observing the sample with an oil lens, wipe the oil lens and carrier with xylene to prevent cedar oil from staining the other objective glasses. Xylene is toxic, wash your hands immediately after use.

11. After the experiment, take out the slide, wipe the lens with lens polishing paper and move it away, not opposite the light hole. Wrap it in silk cloth and put it back in the case. Do not expose the microscope to direct light.

1. Take the mirror and place it.

1 Hold the arm with your right hand and hold the base with your left hand.

2 Place the microscope on the bench, slightly to the left (the microscope is about 7 cm away from the edge of the bench.

place). Attach the eyepiece and objective.

2. Alignment 3 Rotate the converter to align the low-magnification objective with the clear aperture (the front end of the objective lens and the stage should be kept at 2 centimeters.)

meters).

4 Align a larger aperture at the aperture. The left eye is fixed inside the eyepiece (the right eye is open for later.)

Draw at the same time). Turn the mirror so that the light is reflected into the barrel through the aperture. Through the eyepiece, yes.

See the white vision.

3. Observation 5 Place the slide specimen (which can also be made from a thin piece of paper with the word "6" printed on it) to be observed.

On the stage, press with a tablet clamp and make the specimen directly facing the center of the light hole.

6 Turn the coarse collimation spiral so that the lens barrel is slowly lowered until the objective lens is close to the slide specimen (eye.

Keep your eyes on the objective lens so that it doesn't touch the slide specimen).

7 Look the left eye into the eyepiece, and at the same time turn the coarse quasifocal spiral in the opposite direction so that the lens barrel slowly rises and straightens.

Until you can see the image clearly. Then turn the fine collimation spiral slightly to make the image of the object seen more clearly.

Precautions: After the experiment, wipe the surface of the microscope clean. Turn the converter to offset the two objectives to the sides and place the lenses.

The barrel slowly descends to the lowest point. Finally, put the microscope in the case and return it to its original place.

From the eyepiece, you can see that the object you want to observe is on the left, and to move it to the middle, the slide should go to (

Left. Edge shifting.

The microscope is a delicate and valuable instrument, and it should be used in strict accordance with the operating procedures, and the following points should be noted:

1) When taking the microscope, you must hold the lens arm with one hand and the lens holder with the other hand, close to the front chest, and place the flat end flat, and do not extract it with one hand, so as to avoid the parts falling off (especially the eyepiece is easy to slip).

2) Do not take out the eyepiece at will to prevent dust from entering the lens barrel, and it is strictly forbidden to disassemble the parts to prevent damage.

3) If the optical part is unclean, you can wipe it with lens paper to wipe the laughing wheel, and do not wipe it with handkerchiefs, fingers or other paper to avoid damaging the lens; If there is dust in other parts, you can wipe it off with a silk cloth.

4) Prevent water, alcohol and corrosive chemicals from contaminating the microscope.

5) The use of the microscope should follow the operating procedures of using the base magnification lens first and then the high magnification lens.

6) When using the microscope, you must keep both eyes open, use both hands at the same time, observe and touch the nucleus, record and draw at the same time, etc.

7) After using the microscope, lower the stage first, remove the slide specimen, and put it back in place. Rotate the converter so that each objective lens is not facing the light hole, and then raise the stage to fix the lens on the stage, straighten the mirror surface of the mirror, and lower the condenser. Finally, wipe all parts clean, check the parts for defects, and then put them back in the case.

1. Take the mirror and place it.

1. Hold the temple arm with your right hand and drag the lens base with your left hand.

2. Place the microscope on the experimental table about 7 cm away from the edge, slightly to the left, and install the eyepiece and objective lens.

Second, to the light. 3. Rotate the converter to align the low-magnification objective with the perforation (keep a distance of 5 to 10 mm between the front end of the objective and the stage).

4. Aim a larger aperture at the perture One eye looks at the eyepiece and the other eye opens Turn the reflector so that the light is reflected into the lens barrel through the aperture The white and bright circular field of vision can be seen through the eyepiece.

3. Observation. 5. Place the slide specimen to be observed on the stage and press it with a tablet clamp, and the specimen should be facing the center of the light hole.

6. Rotate the coarse collimation spiral to slowly lower the lens barrel until the objective lens is close to the slide specimen (at this time, the eyes must look at the objective lens, and the experimenter's eyes should look between the objective lens and the specimen to avoid collision between the objective lens and the specimen).

7. Look inside the eyepiece of one eyepiece, and at the same time rotate the coarse quasifocal spiral in the counterclockwise direction to make the lens barrel rise slowly until you can see the object clearly, and then slightly rotate the fine quasifocal spiral to make the object more clear.

Fourth, wipe and do tremor retraction.

8. If necessary, wipe the eyepiece with the lens paper next to it and put it back in its original place.