Drosophila life history and its feeding management

1. Fruit fly feeding techniques.

Fruit fly feeding equipment.

Milk bottles, large and medium-sized finger tubes or large test tubes are often used for rearing fruit flies. In the laboratory, 100ml, 150ml and 250ml Erlenmeyer flasks and large and medium-sized finger tubes were used for comparative experiments, and cotton balls were wrapped in gauze as stoppers to culture Drosophila.

The results showed that the fruit flies cultured in 250ml Erlenmeyer flasks had good viability and fecundity, and their eggs were large, and the larvae were large and fat.

This indicates that the larger the growth space of the fruit fly, the more conducive to the reproduction of the fruit fly and the growth and development of the individual. Therefore, in order to grow fruit flies quickly and better, a utensil with a larger container can be used.

Preparation of culture media.

Drosophila uses yeast as the main feeding, so any fermentable substrate in the laboratory can be used as a medium for Drosophila, and the commonly used medium for Drosophila is corn flour, rice flour and banana medium.

Its commonly used formula is corn flour-brown sugar-agar medium, and the specific preparation method is as follows:

Water, 75 ml agar, brown sugar, white sugar.

Corn flour benzoic acid (propionic acid).

Step 1: Fill the agar with brown sugar into a 200ml beaker, add another 25ml of water, and stir constantly until boiling. At the same time, mix the corn flour with 50ml of water and pour another beaker to a boil.

Step 2: Mix the two and continue to heat and stir until they are evenly thickened.

In the third step, benzoic acid is added to the mixture after dissolving with 95% alcohol by volume, or propionic acid is added dropwise. After mixing well, it is divided into culture flasks, the thickness of the medium is 2 3cm, and then the sterilized cardboard strips are added as a dry place for pupation.

Step 4, add stoppers to the bottle mouth, wrap and seal it with kraft paper and cotton yarn, and sterilize it at 121 high temperature and high pressure for 15 20min.

Step 5, take out and cool, preferably not hot hands, and then add yeast powder.

Summary. Fruit flies are also relatively easy to raise, with a milk bottle, put some mashed persimmons, you can raise hundreds or even thousands of fruit flies. The second is fast reproduction, which reproduces one generation in about 10 days at a temperature of about 25, and a female fruit fly can reproduce hundreds in a generation.

Parent fruit flies at room temperature less than two weeks, the life cycle is short, at a temperature of about 25 about ten days to reproduce a generation, a female fruit fly generation can reproduce hundreds, growth process: the larvae will molt for the first time after 24 hours, and continue to grow, to the bucket belt to reach the second larval development stage. After three larval development stages and four days of pupal stage, after one day at 25 years, the stake will develop into an adult.

Fruit fly breeding is also relatively easy stove Mingchun, with a milk bottle, put some mashed persimmons, you can raise hundreds or even thousands of fruit flies. The second is fast reproduction, which reproduces one generation in about ten days at a temperature of about 25, and a female fruit fly can reproduce hundreds in one generation.

I hope my above can really help you, I wish you a happy life and all the best!