Can anyone tell me the specific steps for microbial autoclaving? The more detailed the better, thank

Add water to the sterilizer (almost to the barbed wire, don't add too much) Put in the utensils to be sterilized, culture medium, etc., the test tube of the petri dish should be wrapped first, and the mouth of the medium bottle should be tied firmly Cover the pot, if it is an automatic steam sterilizer, you can start it with a good time, if it is manual, you should heat it for a period of time and wait for the air in the pot to drain and then close the high-pressure valve, the time and temperature are the same, and then open it after the temperature drops down after the sterilization is over.

Now it's mostly fully automatic, I don't know what yours is!!

Generally, look at the water level first, if the water is not enough, it will burn out. Generally, it goes to the bottom of the bottom wire frame!!

Open the lid of the pressure cooker >> put it into the sterilization medium>> set the parameters (according to the sterilization requirements of your medium, generally 121, 20min) >set> adjust to the temperature, and when flashing, press the up and down keys to adjust to the desired temperature>> next>Set the time, same temperature" next> set the holding temperature (if you can't get to the plate in time if you have something to do with the solid medium, you can set the insulation) >enter>start.

The operation of different high-temperature steam autoclaves is different, it is recommended that you better find someone around you who has used it to teach you, because it is quite expensive and good tens of thousands of one, and it is troublesome!!

The estimate of our college and the first floor is of the same type, but if you want to sterilize a test tube, you must remember to wrap a layer of newspaper or kraft paper on the outside of the gauze when bandaging, otherwise it may be infected after sterilization. Just pay attention to it. Also, if there is a small kettle with a knot drain underneath, you need to make sure that the water in the kettle is at the optimal water level before starting.

Ultraviolet disinfection.

At room temperature 20 25, the strong lateness of ultraviolet radiation at the vertical position below the 220V 30W UV lamp should be 70uw cm2, and should be replaced when it is lower than this value. The appropriate number of UV lamps to ensure that the average per cubic meter should not be less than.

When disinfecting with ultraviolet light, the sterile room should be kept clean and dry.

Under unmanned conditions, ultraviolet disinfection can be taken, and the action time should be 30min. When the indoor temperature < 20 or 40 and the relative humidity is greater than 60%, the irradiation time should be appropriately extended.

When disinfecting surfaces with ultraviolet light, the irradiated surface should be exposed to direct ultraviolet light, and the irradiation dose should be sufficient.

Personnel should turn off the ultraviolet lamp for at least 30min before entering the operation.

In accordance with the provisions of GB15981, the disinfection and sterilization effect of ultraviolet rays is evaluated.

2.Ozone disinfection.

In a closed sterile room, under unmanned conditions, ozone with a concentration of 20mg m3 should be used, and the action time should be 30min. After disinfection, the indoor ozone concentration can be used before entering the operation.

In accordance with the provisions of GB T18202, the concentration of indoor odor code Qiaoqi oxygen is detected.

3.Method for verifying the effect of air sterilization in clean room (sedimentation method).

Samples are taken after disinfection and before testing activities are carried out.

The selection of sampling sites should be based on the flow of people and the frequency of the test. Under normal circumstances, when the area of the clean room is 30m2, select 3 points from a set diagonal, that is, 1 point in the center and 1 point at each end 1m away from the wall; When the area of the sterile room is 30m2, 5 points are selected in the east, south, west, north and middle, and the eastern, southern, western and northern points are all 1m away from the end.

At the selected site, the plate counting agar plate (90 mm) or the hydrated 3 m Petrifilm total colony count test piece was placed at a distance of 80 cm from the ground, the lid was opened and exposed for 15 min, and then the plate was placed in a 36 Shi 1 incubator for 48 h and soil for 1 h. If a target bacterium is detected, selective agar plates (e.g., PDA plates) or microbiological plates (e.g., 3 M Petrifilm environmental Listeria plates) can be used.

Confirm the number of colonies on the plate, if it is greater than the set risk value, the cause should be analyzed, and appropriate measures should be taken.

Theoretically, there are no microorganisms left alive after autoclaving, but they still need to be disposed of according to medical waste and put into yellow garbage bags.

Hello, according to your problem, close the air release valve, after the power is on, when the pressure rises, open the air release valve, release the air, and then close the air release valve after the pressure gauge pointer is zero. After the valve is closed and the power is turned on, the pressure gauge rises to the point when the brother arrives, and the timer starts to maintain the pressure for 20 minutes. After reaching the holding time, the power supply can be cut off, and the vapor can be slowly released when the pressure drops, and care should be taken not to reduce the pressure too quickly, so as to cause intense decompression boiling, so that the liquid in the container overflows.

When the pressure drops to zero, the lid can be opened, the medium is removed, and placed on the platform to wait for condensation. Do not deflate for a long time, which will cause changes in the composition of the medium, so that the medium cannot swing the slope. Once placed for too long, due to the negative pressure in the boiler beam, the lid can not be opened, as long as the air release valve is opened, the atmosphere is pressed, and the internal and external pressure is balanced, the lid is easy to open.

For items that do not deteriorate after autoclaving, such as sterile water, cultivation media, and inoculation utensils, liquid dust transportation can extend the sterilization time or increase the pressure. The medium should strictly adhere to the holding time, not only to maintain the pressure thoroughly, but also to prevent the deterioration of the components in the medium or the reduction of effectiveness, and not to extend the time at will. I hope I can help you, and I hope you can give me a thumbs up.

Hello friends, autoclaving, also known as autoclaving, quietly needs a certain sterilization equipment, that is, autoclave Tong Lao. It is proportional to the temperature of the steam and the steam pressure, through the pressure to increase the temperature, the burial of the wheel with high temperature steam for sterilization, sterilization, thorough, the best effect and can greatly shorten the sterilization time, is the most effective, the most widely used sterilization method.

Atmospheric steam sterilization is currently the most widely used sterilization method in the large-scale production of edible fungi, which can be used in steamers, steamers, iron buckets, soil steaming stoves, etc. The capacity can be large or small, and the structure can be adapted to local conditions, which is economical and practical. When sterilizing, bottles and bags should be placed in an orderly manner and leave a certain gap, and sterilize with 100 circulating steam.

The temperature of the circulating steam generally does not exceed 100, so it can only kill microbial propagules, and cannot completely kill spores and spores. Atmospheric steam sterilization must take more than 8 hours or more. At the end of sterilization, the stove door can only be opened when the temperature drops to 70 dust and the mu mold is sent to the mu, otherwise it is easy to cause the bag to be thrust and scrapped.

The disadvantages of atmospheric sterilization are that the sterilization time is long, the energy consumption is large, the water is easy to boil dry or need to be continuously added, and the slightest carelessness is easy to cause incomplete sterilization.

Hello friends, autoclaving, also known as autoclave, requires certain sterilization equipment, that is, autoclave. It is proportional to the temperature of the steam and the steam pressure, through the pressure to increase the temperature, the use of high temperature steam for sterilization, sterilization, sterilization, the best effect and can greatly shorten the sterilization time, is the most effective, the most widely used sterilization method. Atmospheric steam sterilization is the most widely used sterilization method in the large-scale production of edible fungi at present.

The capacity can be large or small, and the structure can be adapted to local conditions, which is economical and practical. When sterilizing, bottles and bags should be placed in an orderly manner and leave a certain gap, and sterilize with 100 circulating steam. The temperature of the circulating steam generally does not exceed 100, so it can only kill microbial propagules, but cannot completely kill spores and spores.

Atmospheric steam sterilization must take more than 8 hours or more. After the end of sterilization, the stove door can only be opened when the temperature drops below 70, otherwise it is easy to cause the bag to be thuffed and thinned and scrapped. The shortcomings of atmospheric pressure sterilization are that the sterilization time is long, the energy consumption is large, the water is easy to boil dry or need to be continuously added, and the slightest carelessness is easy to cause incomplete sterilization.

The autoclave is changed to constant jujube cherry pressure sterilization, and it is not pressurized.

Autoclave. Sterilization is to kill the original bacteria in the medium itself or the miscellaneous bacteria attached to the air, so as to better let the target bacteria of orange potatoes grow (reduce the consumption of nutrients in the medium by miscellaneous bacteria, and some miscellaneous bacteria will inhibit the growth of the target bacteria), generally after sterilization, the medium will be placed in an environment of 30-37 degrees for a day to see if there are any long bacteria (liquid to see whether it becomes turbid, solid to see whether it has long colonies), that is, to know whether it is sterile after sterilization.

Steps: 1. Energized:

Close the leakage protection switch on the left side of the machine (the handle is facing up to indicate that it has been closed), the instrument automatically performs a self-test, the screen and all the indicators flash three times, if the cover is not closed tightly at this time, the screen "lid" flashes on the lower left, and the cover is closed awake; If the lid is closed at this point, the "st-by" light flashes to indicate that it has entered standby mode.

Two. Check whether the sealing ring is sticky: if Li Chanyou has it, it should be separated so as not to affect the seal.

Three. Check the water level of the front water tank, keep the water level of the front tank below the high water level, and empty it if it is half way from the high water level.

5. Close the cover: Gently press the middle of the front end of the lid with one hand, and dial the handle from the right "unlock" to the left "lock" with the other hand. After dialing in place, the system will sound a prompt sound, and the "lokced" light in the upper left corner of the operation panel will turn on at the same time (indicating that it has been turned off).

At this point, the "st-by" light of the screen status flow chart flashes to indicate that it has entered the standby state.

6. Select the program: Press the "up" key and the "down" key to select the program, then the lower left display will be displayed.

U01, U02, etc.

7. End of sterilization: The system emits 5 long sounds, and at the same time, the "COMP" light on the status flow chart flashes, indicating the end of sterilization.

8. Open the cover: Gently press the middle of the front end of the lid with one hand, and dial the handle from the left "lock" to the right "unlock" with the other hand. At this point, the bottom left screen "lid" flashes. Grasp the handle, open the lid, and remove the sterilization.

9. At the end of the day's sterilization work, the power switch should be turned off, and it is recommended to drain the water in the sterilization chamber and water tank.

1 First take out the inner sterilization bucket, and then add an appropriate amount of water to the outer pot to make the water surface level with the triangular shelf.

2 Put back the sterilization bucket and fill it with the items to be sterilized. Be careful not to overcrowd it so as not to obstruct the steam flow and affect the sterilization effect. Do not contact the wall of the barrel with the end of the triangular flask and the mouth of the test tube, so as to prevent condensation from wetting the paper at the mouth and penetrating the tampon.

3 Attach the cap and insert the exhaust hose on the lid into the exhaust groove of the inner sterilization bucket. Then tighten the two opposite bolts at the same time in a symmetrical way, so that the bolts are tightened consistently, and do not leak air.

4 Heat with an electric stove or gas and open the exhaust valve at the same time to bring the water to a boil to remove the cold air from the pot. When the cold air is completely drained, close the exhaust valve and let the temperature in the pot gradually rise as the steam pressure increases. When the pressure in the pot rises to the desired pressure, control the heat source and maintain the pressure for the required time.

This experiment was sterilized with 1 05 kg cm2, 121 3, 20 minutes.

5. When the pressure of the pressure gauge drops to 0, open the exhaust valve, loosen the bolts, open the cover, and take out the sterilized items. If the pressure does not drop to 0, open the exhaust valve, the pressure in the pot will drop suddenly, and the medium in the container will rush out of the mouth of the flask or test tube due to the imbalance of internal and external pressure, causing the tampon to contaminate the medium and contaminate.

6 Put the sterilized medium into the 37 incubator for 24 hours, and if there is no growth of miscellaneous bacteria, it can be used after inspection.

Autoclave sterilization is a sterilization method that kills all microorganisms, including spores, and is the best sterilization effect. It is suitable for the sterilization of ordinary culture media, normal saline, surgical instruments, glass containers, syringes, dressings and other items.

There are two categories: lower row pressure steam sterilizer and pre-vacuum pressure steam sterilizer. The lower row autoclave includes two types: portable and horizontal. (1) The lower exhaust pressure steam sterilizer, the lower part has an exhaust hole, and the cold air is discharged from the bottom exhaust hole with the help of the steam pressure on the upper part of the container by using the difference in the specific gravity of the cold and hot air during sterilization.

The temperature, pressure, and time required for sterilization vary depending on the type of sterilizer, the nature of the item, and the size of the package. When the pressure is in, the temperature can reach 121 126, and 15 to 30 minutes can achieve the purpose of sterilization.

2) Pre-vacuum pressure steam sterilizer, equipped with a vacuum pump, before the steam is introduced, the inside is pumped into a vacuum to form a negative pressure to facilitate steam penetration. At pressure, the temperature reaches 132 134 and can be sterilized in 4 minutes.

3 Precautions.

1) The sterilization package should not be too large or too tight (the volume should not be greater than 30cm 30cm 30cm), and the total amount of items placed in the sterilizer should not exceed 85% of the volume of the sterilizer cabinet. There is a gap between the bags to facilitate the circulation of steam and infiltrate into the package**, and the steam is quickly discharged when venting to keep the items dry.

2) The container containing the items should have holes, and if there are no holes, the lid of the container should be opened.

3) Cloth items are placed on top of metal and enamel items.

4) The sterilized items should be dried before they can be taken out for later use.

5) Before the sterilizer is closed, the cold air should be fully emptied.

6) Observe the pressure and temperature at any time.

7) Pay attention to safe operation, and check whether the sterilizer is in good working condition before each sterilization.

8) After the sterilization is completed, the decompression should not be too violent, and the lid or door can only be opened after the pressure gauge returns to the "0" position.