Ask for help in the process of making blood agar medium in large quantities

Updated on delicacies 2024-04-08
5 answers
  1. Anonymous users2024-02-07

    According to the standard 250g Fraser two-step multiplication, 19 samples can be made, so the preparation amount of 250g Fraser FB2 is 190ml

    For another example, there are some biochemicals that are used more, and I don't think it's appropriate to buy biochemical tubes, so I buy culture medium. The amount of media available is too large and requires very little for each preparation. For example, MRVP, VP, U, TSI, NACLTSI, KIA, T1N1, T1N0, ordinary broth, 42 degrees growth and other media, all packaged are 250 grams.

    Mine hasn't used half of it yet, and it's expired. It was said that it could be used again without clumping, but I felt uncomfortable in my heart. It's even more troublesome to run into inspections.

    Therefore, it is not possible to change such a medium to a 100g pack. Even if the packaging fee is a little more expensive than 250 grams, it is cheaper than that, and it is not so distressing to replace it after expiration.

    According to the standard 250g Fraser two-step multiplication, 19 samples can be made, so the preparation amount of 250g Fraser FB2 is 190ml

    For another example, there are some biochemicals that are used more, and I don't think it's appropriate to buy biochemical tubes, so I buy culture medium. The amount of media available is too large and requires very little for each preparation. For example, MRVP, VP, U, TSI, NACLTSI, KIA, T1N1, T1N0, ordinary broth, 42 degrees growth and other media, all packaged are 250 grams.

    Mine hasn't used half of it yet, and it's expired. It was said that it could be used again without clumping, but I felt uncomfortable in my heart. It's even more troublesome to run into inspections.

    Therefore, it is not possible to change such a medium to a 100g pack. Even if the packaging fee is a little more expensive than 250 grams, it is cheaper than that, and it is not so distressing to replace it after expiration.

    According to the standard 250g Fraser two-step multiplication, 19 samples can be made, so the preparation amount of 250g Fraser FB2 is 190ml

    For another example, there are some biochemicals that are used more, and I don't think it's appropriate to buy biochemical tubes, so I buy culture medium. The amount of media available is too large and requires very little for each preparation. For example, MRVP, VP, U, TSI, NACLTSI, KIA, T1N1, T1N0, ordinary broth, 42 degrees growth and other media, all packaged are 250 grams.

    Mine hasn't used half of it yet, and it's expired. It was said that it could be used again without clumping, but I felt uncomfortable in my heart. It's even more troublesome to run into inspections.

    Therefore, it is not possible to change such a medium to a 100g pack. Even if the packaging fee is a little more expensive than 250 grams, it is cheaper than that, and it is not so distressing to replace it after expiration.

  2. Anonymous users2024-02-06

    If you want the color to look good, you can really consider additives, as if it were some kind of acid. However, the toxicity of the additives used to the culture needs to be considered.

  3. Anonymous users2024-02-05

    Pay attention to adjusting the pH value, it is better to adjust it to about four, and then adjust it to 8 to nine.

  4. Anonymous users2024-02-04

    Blood agar medium is a medium widely used in bacteriological research, which can isolate, identify and analyze the pathogenicity of staphylococci and streptococci by preparing agar medium containing hemoglobin.

    For staphylococcus, the characteristic of blood agar medium is that it can promote the production of hemolysin in staphylococcus, so it can make staphylococcus produce obvious hemolysis phenomenon, so as to accurately detect the pathogenicity of Staphylococcus grapevine. At the same time, the blood agar medium can also detect other congeners such as lectins and proteases produced by staphylococcus.

    In the case of streptococcus, the pathogenicity of blood agar medium can be determined by testing its hemolysis on the medium. On the blood agar medium, the hemolysin produced by streptococcus can lyse red blood cells to form a hemolytic ring, which can be used to identify the pathogenicity of Bibidine streptococcus. In addition, streptococci grow rapidly on blood agar medium, which allows for rapid detection of pathogenicity.

    In summary, blood agar medium is an important tool for the analysis of staphylococcal and streptococcal pathogenicity, and the pathogenicity of bacteria can be accurately identified by detecting the hemolysis of bacteria on the medium and the production of other pathogens.

  5. Anonymous users2024-02-03

    Blood agar medium is a nutrient agar medium used to culture and recognize bacteria. It is a widely used medium that is often used in areas such as clinical diagnosis and pathogen identification. Blood agar medium promotes the growth of a wide range of bacteria, as well as rapid detection of aerobic and anaerobic bacteria.

    So, what bacteria can be used to isolate blood agar medium?

    Blood agar medium can be used to isolate many finches to make different species of bacteria. These include gram-positive and gram-negative bacteria such as streptococcus, pneumococcus, Staphylococcus aureus, enterococci, etc. In addition, the commonly used blood agar medium can also isolate anaerobic bacteria, such as Clostridium perfringens and anaerobes.

    Among them, Clostridium perfringens and anaerobic bacteria are common bacteria that cause gastrointestinal infections, so the corresponding detection methods are also particularly important.

    Blood agar medium is a nutrient-rich agar medium. It contains a variety of vitamins, amino acids, and other important growth factors to support the growth of various types of bacteria. In addition, blood agar medium also contains red blood cells or plasma, which can provide various nutrients needed for bacterial growth, further promoting the growth of various types of bacteria.

    In addition to its wide range of applications, blood agar medium has certain limitations. For example, the medium is not effective in treating bacteria that are inhibited by antibiotic transport and unconventional bacteria. In addition, if the medium contains too many red blood cells or plasma, it can also have an adverse effect on the growth of the cells.

    Therefore, in practical applications, we need to make appropriate adjustments according to the specific situation to obtain more accurate test results.

    In conclusion, blood agar medium is a commonly used bacterial medium. It is widely used in the fields of clinical diagnosis and pathogen identification. The base can isolate many different types of bacteria, including some bacteria that maintain human health, as well as certain pathogenic bacteria.

    Of course, there are certain limitations when using blood agar medium. Therefore, we need to make reasonable adjustments accordingly to obtain more accurate and reliable test results.

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