Do you know how to collect sheep disease?

The collection of disease materials includes: when the acute death of sheep is found before autopsy, it must be examined with a microscope for the presence of anthrax bacillus in the peripheral blood smear. If anthrax is suspected, an autopsy should not be performed and only if it is determined that it is not anthrax.

The collection of dirty and sick materials within the time of collection must be carried out immediately after death, preferably no more than 6 hours, otherwise the time is too long, due to the invasion of other bacteria in the intestines, it is easy to decompose the corpse, affecting the accuracy of the detection of pathogenic microorganisms. Sterilizing knives, scissors, tweezers, syringes, needles, etc. of instruments should be boiled for 30 minutes. Utensils (glass, pottery, enamel, etc.) can be autoclaved or dry-baked.

Cork stoppers and rubber stoppers are placed in an aqueous solution of carbolic acid and boiled for 10 minutes. Take 1 kind of disease material, use 1 set of instruments and containers, and do not mix. The collection of disease materials should be based on different infectious diseases, and the organs or contents that are often affected by the disease should be taken accordingly.

For example, septicemia infectious diseases can take heart, liver, spleen, lungs, kidneys, lymph nodes, stomach, intestines, etc.; Enterotoxemia takes the small intestine and its contents; Infectious diseases with neurological symptoms take the brain, spinal cord, etc. If it is not possible to determine what kind of infectious disease it is, we can take full measures. When serum antibodies are checked, blood is taken, the serum is precipitated after coagulation, and the serum is sent to a human sterilized vial for testing.

In order to avoid contamination by miscellaneous bacteria, the examination of the lesion should be carried out after the collection of disease materials. Pus, blood and mucus smears for microscopic examination can be made by the following method of pushing the slide: the material is placed on the glass slide first, and then evenly applied with a sterilized glass rod or pushed with the edge of one end of another slide at a 45° angle to the slide; When using a tissue block as a contact piece, you can gently apply the free side of the tissue block to the glass slide with a small deterrent.

The number of the smear, touch, bandage, and glass slide should be marked with a separate description.

The collection of sick sheep is as follows: the fresh disease material for laboratory testing must be fresh. Bodies that are in decay and deterioration cannot be collected.

Bacteriological examination should collect dead sheep without antibiotics**. Instruments used for sterile sampling are autoclaved beforehand. The surface of the sampling site is disinfected with alcohol or flame.

The container or sampling bag in which the sample is stored needs to be sterile. Try to avoid contamination of the sample during the procedure. The storage of samples should be based on the epidemic situation.

Generally, the lungs, pleural effusion, pericardial effusion, liver, lymph nodes, brain tissue, etc. are collected. Pathogen examination was done, and the disease material was stored with 50% sterilized glycerol and normal saline. Mucosal samples scraped from the esophagus and pharynx of animals with 0 p solution are mainly used to investigate the infection of foot-and-mouth disease and poison in ruminants such as cattle and sheep.

The animals were fasted for 12 hours in advance (drinking water) and collected with a special esophageal probe cup.

When there is a suspicion of an infectious disease in sheep, in addition to confirming the diagnosis according to the clinical manifestations and pathological autopsy, some infectious diseases also need to be sent to the veterinary quarantine and epidemic prevention department for microbiological testing in a timely manner. The diseased material should be fresh, and it is best to take it within 2 hours before or after the death of the sick sheep; The use of sick materials should minimize the contamination of miscellaneous bacteria, and the instruments should be strictly disinfected in advance to achieve aseptic collection; For sick sheep that endanger human health, it is necessary to pay attention to personal protection and avoid spreading poison. When it is difficult to estimate the type of infectious disease, all organs and tissues of the whole body or diseased tissues can be used; Specialized infectious diseases or infectious diseases dominated by a certain organ should be organized accordingly; The aborted fetus or lamb can be packaged and sent for testing; It is strictly forbidden to dissect the sick sheep suspected of anthrax, but the ear tip blood smear can be sent for testing, and the serum should be collected to prevent hemolysis, and each sheep should take 10 20 ml of whole blood, and the serum will be separated after standing.

The diseased material should be fresh, and it is best to take it within 2 hours before or after the death of the sick pig; The sick materials should be made as much as possible to reduce the contamination of miscellaneous bacteria, and the instruments should be strictly disinfected in advance to achieve aseptic collection; For sick pigs that endanger human health, it is necessary to pay attention to personal protection and avoid spreading poison. When it is difficult to estimate what kind of infectious disease, various organs and tissues of the whole body or lesions can be used. Specialized infectious diseases or infectious diseases dominated by a certain organ should be organized accordingly; The aborted fetus or lamb can be packaged and sent for testing; It is strictly forbidden to dissect the suspected anthrax pigs, but the ear tip blood smear can be sent for testing, and the serum should be collected to prevent hemolysis, and each pig should take 10 20 ml of whole blood, and the serum should be separated after standing pure trouble.