What kind of gelatin is used to promote the attachment of biological cell cultures? 5

Cell culture media:

Zhejiang Weikang brand cell culture medium includes MEM medium, DMEM medium, DMEM F12 medium, RPMI1640 medium, MEM medium, IMDM medium, M199 medium, and MCCOY'S 5A medium, HAM'S F-10 medium, HAM'S F-12 medium, HAM'S F-12K medium and Leibovitz'SL-15 medium, etc.

2.Disposable ready-made sterile medium with less nutrient loss and ready to use right out of the bottle.

3.Verified by multiplex cell testing, the culture quality is reliable, and the variability between batches is small.

4.In stock**, a variety of component combination cell culture media can be customized according to customer needs.

5.Filter membrane filtration sterilization, no pollution; Formulated with water for injection and low endotoxin.

Zhejiang Weikang Medical Technology Co., Ltd. is committed to developing a high-quality, effective and stable basic cell culture medium, providing suitable pH, osmotic pressure and nutrients for cell growth, so that cells can maintain a good state in an artificial simulation environment in vitro. Cell basal medium series products include MEM medium, DMEM medium, DMEM F12 medium, RPMI1640 medium, MEM medium, IMDM medium, M199 medium, and MCCOY'S 5A medium, HAM'S F-10 medium, HAM'S F-12 medium, HAM'S F-12K medium and Leibovitz'SL-15 medium, as well as a variety of customizable media, are suitable for in vitro culture of a variety of suspended and adherent mammalian cells and cell lines.

There are other collagens that have rat tail gum as well

q1.Problems with gelatin solution configuration

A1: Use deionized water to prepare. If the domestic one works or not, you have to test it, and the insurance is to buy the imported one.

In fact, it is usually good to buy Sigma's gelatin powder to match, and there is no need to buy ready-made powder. The prepared concentrate can be stored at -20 degrees for a long time, and the concentrate to be used can be stored at 4 degrees for later use.

q2.The problem of the concentration of gelatin used

A2: 1% is the concentration of concentrated mother liquor, and an appropriate amount is diluted before use to coat.

q3.Problems with gelatin disinfection:

A3: Gelatin is sterilized by high temperature and high pressure, and the other methods you mentioned are not unusable, but they are not necessary.

q4.Problems with gelatin-coated culture flasks

A4: Usually 1 hour of coating is enough, and then blotting dry, no need to clean. Because your gelatin solution doesn't contain any other harmful substances, rinsing is just an unnecessary job. Cleaning after coating is only needed for collagen.

q5.The problem of the service life of the culture flask after gelatin coating

a5.I don't know about this, but basically the sooner you use it, the better. Since the coating process is very fast, I always coat on the same day, and I can do other things while waiting, start processing the cells 20 minutes before the coating is about to be completed, and when the cells are collected, the coating is ready, and I will use it immediately.

Gelatin is suitable for coating most cell cultures and does not gelatinize when placed at four degrees. The literature I refer to says that endothelial cells are coated with gelatin, i.e., mother liquor, which coagulates moderately when placed in four degrees. I think my endothelial cells are coated with 1 gelatin, and I think it's pretty good.

Regardless of whether you use the 1-hour coating method or the 4-hour coating method, it is best to have no more than one day between the start of the coating and the use of the container, and the longer the coated container is placed, the worse it is.