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In most cases, cells need to be cultured in vitro, but the serum contains exosomes, in order to avoid serum contamination of the exosomes produced by cells, two methods are generally used: Before cell culture, pass 100,000?Ultracentrifuge overnight to remove serum exosomes.
Umbio serum-free medium can be selected for culture. Yumeibo Biotech is providing free exosome extraction kits on a first-served, first-served basis.
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Hello. Because the membrane permeability of dead cells has changed, they no longer have the selective permeability of the cell membrane of living cells.
Therefore, the plasma-wall separation of dead cells is very insignificant.
Hope it helps.
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Plasmo-wall separation is a phenomenon that is unique to mature plant cells.
Parietal refers to the cell wall.
Plasta refers to the protoplasmic layer, which is composed of a vacuolar membrane, a cell membrane, and cytoplasm between two membranes.
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What cells? Animal cells? Animal cells do not have a cell wall how can the plasma wall be separated.
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Plasma wall separation refers to the phenomenon that the cell protoplasm shrinks and separates from the cell wall due to water loss and contraction due to water loss when the concentration of the surrounding solution is higher than the concentration of the cell liquid, and because the cell wall deformation ability is less than that of the cytoplasm.
Based on the above information, first of all, we can know that the cell that can undergo plasmo-wall separation must have a cell wall. Furthermore, as a permeable system, there are two conditions that should be met: there is a concentration difference between the semi-permeable membrane and the two sides of the membrane.
In cells where plasma wall separation occurs, there are three layers of structures that act as semi-permeable membranes: the liquid hand bubble membrane, the cytoplasm, and the cell membrane. It can be seen that cells capable of plasmo-wall separation also need to have large vacuoles.
In summary, only cells with cell walls and large vacuoles can have plasmo-wall separation.
Such cells are mature plant cells (plant cells with large vacuoles).Such as: mesophyll cells, leaf epidermal cells, onion scale epidermis fine sedan cells, etc.
Immature plant cells, such as the cells in the apical meristem and the cells in the seeds, cannot be separated from the plasma wall.
A high school biology teacher,
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The separation of cell wall and cell membrane plasma wall is caused by the different concentrations of Na+ and K+ ions inside and outside the cell, and the rapid dismantling of cells flows from low concentration to high concentration.
The outflow of cell fluid leads to the separation of the plasma wall and the decrease of intracellular fluid, resulting in the separation of the cell membrane from the cell wall Sorry, I didn't see the problem clearly Said the wrong The cell wall and the cell membrane are not handy, and the prosper is an organelle, and the vacuole is.
Animal and plant cells share common organelles: endoplasmic reticulum, Golgi apparatus, mitochondria, ribosomes, lysosomes, plastids (not only in plants).
Plant endemics: chloroplasts, spindles, vacuoles.
Animal endemic: centrosomes.
Those that do not belong to the microbeam organelles: the cell wall, the cell membrane, the nucleus and its internal structure.
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1.Plant cell walls are less elastic, whereas protoplasmic layers are more elastic. 2.
When the concentration of the external solution is greater than the concentration of the cell fluid, the implanted cell loses water through osmosis, showing the separation of the plasma wall. When the concentration of the external solution is less than the concentration of the cell fluid, the plant cells absorb water through osmosis and gradually show the phenomenon of recovery after the separation of the plasma wall.
What are the conditions under which cytoplasmic wall separation occurs1.The concentration of the vesicle fluid is the concentration of the external solution.
2.The cell wall is fully permeable.
3.The protoplasmic membrane composed of cytoplasm, cell membrane, and vacuolar membrane is semi-permeable and selectively permeable, equivalent to a semi-permeable membrane.
4.The protoplasmic layer is more elastic than the cell wall.
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When the concentration of the plant extracellular solution is greater than the concentration of the plant intracellular solution, the phenomenon of plasma wall separation occurs in the plant cell.
The reason for this phenomenon is actually very simple, and it is not worth mentioning to mention. That is related to the absorption and loss of water by cells.
In the case of a plant cell, the outermost part is the cell wall, the inner part is the cell membrane, and the inner part is the cytoplasm, in addition to the nucleus.
The cell wall of the plant is fully permeable, any substance can pass through, and the elasticity is relatively smallHowever, the cell membrane is a selectively permeable membrane, that is, a semi-permeable membrane, which can only allow the passage of the manuscript imitation car-like substance, and the elasticity is greater than that of the cell wall.
Therefore, when we put the rotten plant cells in a high-concentration sucrose solution, due to the large concentration of the external solution, the water in the cells will seep out, causing the protoplasmic layer to shrink and separate from the cell wall, which is the so-called plasma wall separation.
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Yes, because only living cells have a selective permeability to the cell membrane, and plasmo-wall separation can occur. The cell membrane of dead cells loses its selective permeability, so the cell cannot undergo plasmo-wall separation. At the same time, you can also judge the life and death of cells through the phenomenon of plasma wall separation and restoration, why, draw inferences yourself.
a. Only living cells can have plasma wall separation, and dead cells will not have plasma wall separation, so the death and survival of mature plant cells can be judged through plasma wall separation experiments, a is correct;
b. The principle of the plasma-wall separation and the sediment-wall separation and restoration experiment is osmosis, and b. is correct;
c. The internal cause of plasma wall separation is that the elasticity of the cell wall is much smaller than that of the protoplasm layer, and the external reason is that the concentration of cell fluid is less than that of extracellular fluid, which is correct;
d. The composition of the protoplasm layer cannot be found in the plasma separation experiment, and the composition of the protopplasm layer requires microscopic technology, and D is wrong
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The reason is that the cell is in the hypertonic solution, because the internal osmotic pressure is less than the external osmotic pressure, so the water will move outward from the cell, after a period of time, the water in the vacuole will be reduced, the cell volume will become smaller, because the elasticity of the cell wall is less than the elasticity of the protoplasm layer, so the cell will occur the phenomenon of separation of the cell wall and the protoplasmic layer.
The phenomenon of plasmo-wall separation of cells can only occur in living cells. In dead cells, plasmo-wall separation does not occur because the cell membrane loses its selective permeability.
Therefore, it is possible to determine the survival of cells by using the plasma wall isolation experiment of cells.
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Plasma wall separation experiments can determine the survival of cells because by definition, dead cells, animal cells, prokaryotic cells, and immature plant cells do not undergo plasma wall separation. Only mature plant cells with ** large vacuoles can have plasmo-wall separation. Therefore, it is possible to determine the survival of cells by means of plasma-wall isolation experiments.
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