What about HPLC HPLC from Agilent, Hitachi, and Shimadzu?

Sympathize with you. It is recommended that you check the advantages and disadvantages of Agilent, the old brand, many 211 projects are Agilent's, Agilent's biggest advantage is the humanized design, and its ** is also more reasonable, generally not easy to have problems, even if there is a problem, it is easy to solve it yourself, unlike other brands, if there is a problem, you have to ask for after-sales, I believe that you will be responsible for solving the problem of the instrument in the future. Hitachi's don't need to be considered, it's not good in all aspects, and it has a bad reputation.

The equipment purchased by our school is Agilent, including gas phase and liquid phase. Shimadzu's is average, and it is easy to use, but the design is not as good as Agilent's, and it feels like it has been used, especially the workstation is not easy to use.

Waters' software applications are abundant, the hardware quality is also good, and the machine and after-sales are often expensive, which is suitable for high-end scientific research;

Agilent software specifications, the quality of the machine is average, but they are all made in Germany, the machine is moderate, the after-sales gray is often expensive, suitable for the standard, the implementation of international certification GLP and GMP laboratory;

Shimadzu's machine software has a single function (relatively speaking), the hardware is solid, and the after-sales service is moderate, which is suitable for domestic enterprises.

Hitachi's wood used to say.

Hehe. Tell your boss that.

Agilent is good, good-looking, good

Hitachi Market is not doing well, there are few people who use it, and all have little reputation.

Shimadzu is cheap, the leather is solid, there are many application articles, there are problems, it is convenient to find someone to communicate, and there are a lot of sales.

If it's not for propaganda and promotion, let's get Taiwan Shimazu, hehe, I declare that I don't sell liquid phase.

Usually I buy waters, and we have several in our lab. I haven't used many other brands, but I've heard that HPLC is the best for Agilent. I've heard of Shimazu, too... Hitachi's I don't know.

Shimadzu's NEXERA UHPLC HPLC next-generation UHPLC is an all-in-one LC system that combines revolutionary technology with excellent ultra-fast LC analysis performance and flexibility, while supporting a wide range of analytical applications, from conventional analytical systems to green LC analysis with reduced organic solvent usage.

1.Technological innovation: Shimadzu is one of the world's leading manufacturers of scientific instruments, with advanced technology research and development capabilities and rich innovation experience.

They continue to introduce innovative new products, and have independent intellectual property rights in many fields, such as analytical instruments, biochemical instruments, chromatographs, etc.

2.Reliable Quality: Shimadzu equipment is known for its high quality and reliability. Shimadzu equipment undergoes strict quality control processes and tests to ensure the stability and accuracy of products. They pay attention to every detail and uphold the principle of providing the best quality products to their customers.

3.Broad product line: Shimadzu covers a wide range of product lines to meet a variety of experimental and research needs. Whether in the field of medical diagnostics, environmental monitoring, or academic research, Shimadzu offers a wide range of instruments and solutions.

4.Customer Service and Technical Support: Shimadzu Equipment excels in customer service and technical support.

They have a global technical support team and a perfect after-sales service network, which can respond to customer needs in a timely manner and provide professional technical support to ensure the normal operation of customers' equipment.

In conclusion, Shimadzu Equipment has become one of the major competitors in the scientific instrument market due to its advantages in technological innovation, reliable quality, extensive product line, customer service and technical support.

The above is provided by Linktour Instruments I hope it will be helpful to you.

Shimadzu's bar, ** has advantages, the quality is OK, the main thing is durable.

The key depends on the size and purpose of your company, if it is just an ordinary test, Shimadzu will do, after all, it is cheap;

If there is a special need, such as the test results need to be recognized by a foreign party, then you have to buy Agilent.

It is recommended to buy Agilent, regardless of the degree of recognition of after-sales or product analysis results, it is better for Agilent.

It depends on your needs.

To contact the sales of the two companies to compare, the waters are actually pretty good.

Used by our company.

Agilent's after-sales service is better, but Shimadzu's doesn't know.

Shimadzu, Agilent did it relatively early in China, Opus Germany, only started in recent years, Agilent is the most expensive, Shimadzu detection limit is very low, but the interface is not friendly, the operation is cumbersome, Opus general,

Opus's cost performance should be relatively high, just entered the Chinese market, and the price will definitely be relaxed.

Agilent gas chromatography is the best in quality and is the leader in the industry. The specific technical indicators of the instrument depend on the model that should be used. You can go to the "World of Chromatography" to see the membership and specific performance parameters of these two instruments.

I don't know what exactly you want to do with liquid chromatography, and what are your requirements for analysis, there are many types of chromatography, different models.

Shimadzu's liquid chromatography is better for preparation, and of course it can also be analyzed.

Agilent's HPLC is recognized as good for analysis of this.

The boss of the liquid phase is recognized as waters

The landlord's question is too simple. What configuration do you want?

High performance liquid chromatograph use.

In the past, high-performance liquid chromatography was often used for content determination in the petrochemical industry, such as pesticides, environmental protection, thin layer chromatography (TLC) and gas chromatography (OC), while liquid chromatography (LC) was only used for the determination of standard samples. and to study the possibility of separation.

The biggest problem in life science research is the decryption of genes. From genomic DNA sequences, gene expression time cannot be answered, expression amount, protein, post-translational processing and modification, and its subcellular distribution. These questions cannot be solved in the genome and it is expected that the answers will be found in the proteome study.

There are between 30,000 and 50,000 proteins with different functions in the cells studied. Currently, the two-dimensional electrophoresis method used in proteomics research can only distinguish between 2000 to 3000 protein spots. Modern proteome analysis can be attempted by pre-separation using 2D HPLC with size exclusion chromatography.

High performance liquid chromatography and two-dimensional electrophoresis will become important separation tools for proteomics. Therefore, high-performance liquid chromatography will make a greater contribution to revealing the mysteries of life.

Imported liquid chromatographs are mainly waters, Agilent, Shimadzu, other non-mainstream, waters is now super bull in ultra-high pressure chromatography, conventional liquid chromatography does not seem to pay much attention, Agilent now occupies the mainstream position among high-end liquid chromatograph customers, Shimadzu is a popular product in imported liquid chromatographs, there are many users, and the products are actually half domestic. Besides, this is a Japanese product, and if you analyze it regularly, it is recommended to replace Shimadzu's with a domestic one!

There are now more brands of domestic liquid chromatographs, the main ones are Elite (the highest in China), Wu Feng, Chuangxin Tongheng, Puxi General, Shanghai Tianmei, Shanghai Hegong, Hangzhou Saizhi and so on.

If it is a general sample isocratic routine analysis, there is no problem with the general instruments in China now.

As far as I know, the imported brands are the United States Thermo Fisher, Agilent, Japan's Shimadzu, Germany's Opus, etc., there are not many domestic famous brands, there is still a gap between technology and imports, and most of all companies still choose to import liquid phase.

Imported liquid chromatography instrument brands: Aupos Scientific (Opus, Germany), Waters (USA), Agilent (USA), Diane (USA), Varian (USA), PE (USA), Sykam (Germany), Scientific Systems SSI (USA), Shimadzu (Japan), Hitachi (Japan), Gilson Gilson (France), Knoll Knaure (Germany), GBC (Australia).

Tailinga Technology will answer for you:

The quality of imported brands is almost the same, compared to the service and cost performance, Agilent and Shimadzu have entered the Chinese market for a long time, and the market share is quite large, the last one or two years German brand Opus has also entered the Chinese market, Opus is the only imported brand with a two-year warranty (including wearing parts) brand, and the instrument is particularly intelligent, can be fully automated, you can learn to operate in one day, ** is also cheaper than other imported brands 1 3

There are many big brands to choose from, such as Thermo Fisher; There are also cost-effective domestic brands, like Sepabean Machine.

Waters' best, but now it's commonly used by Shimadzu and Agilent.

I've used all three, and I like Agilent.

Maybe it's because of Agilent's best-equipped and most expensive ORz

Agilent's bar is good value for money

Of course, the foreign ones are Waters, as well as Platinum Emer and Agilent, Noel's is also good, Shimadzu's is OK, and there are more domestic liquids... Can you get in touch more.

If you don't look at the cost performance, it is waters, and it can be said that waters has always been the leader of liquid chromatographs, so it is not an exaggeration to say that. But it's also relatively expensive. If you consider Agilent and Shimadzu from the cost performance, it's also good.,Usual analysis,Which of these three is actually about the same.。。

These are basically the three major brands imported and the three brands with the largest market share. Noll or something, forget it. Compared with these three, the distance is still very far.

What brand of high-performance liquid chromatograph do you use? I have used Agilent's and Shimadzu's, the quantitative tube 2ml but the actual injection amount is based on your own setting in the instrument, generally 10-20ul, if the pipeline is empty then do not go to the column, first clean the system with ultrasonic purified water, and then choose the solvent of the next cleaning system according to the selected column, if the mobile phase you need is acetonitrile, then use methanol to clean the system, you can also use acetonitrile, but the ** of acetonitrile is 4 times that of methanol, so methanol is generally used, After rinsing, load the column, degass, and then rinse with the desired mobile phase rinse solution (depending on the column). Generally, the volume of the flush is 10 times the volume of the column.

The mobile phase and sample are then applied, degassed, and the sample is ready for injection. (Be sure to degas after any disconnection of the system cycle, otherwise it will cause unstable baseline distortion or ghost peaks or damage to the column) Pay special attention to the selection of the following two column flushes (1Dextran reticulated molecules used as columns should not be cleaned with organic solvents2

Columns with aqueous solvents are prohibited) The former causes the dextran reticulated molecules to rupture, clogging the tubing, and the latter destroying the column. These are extremely troublesome situations. The current instruments are all automatic sampling, and there is basically no possibility of air entering the column after the double insurance of ultrasonic degassing and automatic exhaust of the system.

A chromatogram, in fact, is simply a two-dimensional map of time and an ordinate of an electrical signal.

With HPLC, you can simply imagine that the stationary phase is a hollow, spongy, cylindrical structure where the sample moves in and out of the holes. Because the adsorption capacity of each substance is different, the distance is pulled in the chromatogram.

1. Retention time.

If the same column is used, the same mobile phase, and the same sample is analyzed, then the retention time of this sample should be fixed. Chromatographic peaks with different retention times should show different substances. If you're running reversed-phase chromatography, the further back the chromatographic peak is, the less polarity it has for the substance.

2. Peak area.

This is the parameter you can read in the chromatogram, and the concentration and peak area of the same substance are proportional to the same chromatographic conditions. In other words, if you prepare substance x with a peak area of 10,000 after injection, then you prepare substance x with a peak area of about 5,000 after injection

3. Wavelength. The same sample, the same method, and the same column have different peak areas at different wavelengths. A substance is one that is absorbed at certain special wavelengths. For example, a substance has absorption at 210 nm and 254 nm.

The substance may not be detectable at a wavelength of 280 nm. So an experimental method starts with a wavelength scan.