What is the nature and function of BSA?

Updated on healthy 2024-06-11
7 answers
  1. Anonymous users2024-02-11

    1. Bovine serum albumin.

    BSA), also known as the fifth component, is an albumin in bovine serum that contains 583 amino acid residues.

    The molecular weight is kda, isoelectric point.

    For. 2. Function:

    1. BSA is added to the enzyme digestion reaction buffer to protect the enzyme by increasing the concentration of protein in the solution. Prevents the decomposition and non-specific adsorption of enzymes, which can reduce the denaturation of some enzymes and reduce adverse environmental factors such as heating and surface tension.

    and denaturation caused by chemical factors.

    2. It is also often used in ELISA, such as blocking solution, sample diluent, and enzyme conjugate diluent can be used BSA.

    3. Biochemical research, genetic engineering.

    and drug research.

  2. Anonymous users2024-02-10

    Bovine serum albumin is what we often call BSA

    The role of BSA in the buffer of the endonuclease.

    It is a stabilizer of enzymes, preventing the decomposition and non-specific adsorption of enzymes;

    It can alleviate the denaturation of some enzymes, and can alleviate some adverse environmental factors such as heating, surface tension and chemical factors as for the denaturation mechanism I am not very clear, probably because it has 17 disulfide bonds in its structure, and a sulfhydryl group, the chemical reaction of the sulfhydryl group is very active, and the disulfide bond has the effect of anti-redox, so it can be combined with a variety of cations, anions and small molecules;

    It can prevent the loss of enzymes from adsorbing to the tube wall.

    BSA is generally used as a stabilizer in the preservation and reaction solutions of restriction or modified enzymes, as some enzymes are unstable or have low activity at low concentrations. After adding BSA, it may play a "protective" or "carrier" role, and many enzymes can greatly increase their activity after adding BSA. Enzymes that do not require BSA to be added to BSA are generally not affected.

    For most substrate DNA, BSA allows for more complete digestion and reproducible cleavage. At 37, BSA can make the enzyme more stable when the digestion reaction exceeds 1 h, as many restriction enzymes can only survive under 37 in the BSA-free reaction buffer10"20min or even less. BSA, on the other hand, can bind metal ions and other chemicals in buffers or substrate DNA that inhibit restriction enzyme activity.

  3. Anonymous users2024-02-09

    Agree with the explanation of the 1st floor, it is indeed comprehensive.

  4. Anonymous users2024-02-08

    1. Bovine serum albumin (BSA), also known as the fifth component, is a kind of albumin in bovine serum, containing 583 amino acid residues, molecular weight kda, and isoelectric point.

    2. Function: 1. BSA is added to the enzyme digestion reaction buffer to protect the enzyme by increasing the concentration of protein in the solution. Preventing the decomposition and non-specific adsorption of enzymes can reduce the denaturation of some enzymes and reduce the denaturation caused by adverse environmental factors such as heating, surface tension and chemical factors.

    2. It is also often used in ELISA, such as blocking solution, sample diluent, and enzyme deficiency conjugate dilution.

    3. Biochemical research, genetic engineering and pharmaceutical research.

  5. Anonymous users2024-02-07

    BSA is the abbreviation of Business Software Alliance, Bovine Serum White Reed Songchai Protein, and Bachelor of Agricultural Science.

    1. BSA is the abbreviation of Business Software Alliance, its Chinese name is translated as "Business Software Alliance", which was founded in 1988 and has branches in 80 countries and regions in major regions around the world. BSA opened a representative office in China in 1997 and has set up 800 Whistleblowers of Pirated Software in China**, whose main function is to combat piracy, which it calls "educating consumers to manage software assets and protect software copyrights".

    2. Bovine serum albumin is a globulin in bovine serum, containing 607 amino acid residues, molecular weight is, and isoelectric point is the companion type. Bovine serum albumin has a wide range of applications in biochemical experiments. Sakura accompanied.

    3. BSA is also the abbreviation of Bachelor of Agricultural Science. Agricultural science is a science that studies the natural and economic laws of agricultural development, and is comprehensive because it involves a variety of sciences such as agricultural environment, crop and livestock production, agricultural engineering, and agricultural economy. Forestry and aquatic sciences are also sometimes included in the broad scope of agricultural sciences.

  6. Anonymous users2024-02-06

    Chinese translation of bsabsa.

    The following results are explained by the dictionary provided by Translation.

    BSA Abbre

    1.=Bibliographical Society of America.

    2.=boy scouts of america.

    3.=boy scouts association.

    4.=blind service association (USA).

    5.=Business Software Association [PC] Business Software Alliance.

    Abbreviation abbr

    1.=Bachelor of science in agriculture ѧ

  7. Anonymous users2024-02-05

    1. BS: Bachelor of Science (English: Bachelor of Science, Latin:

    Baccalaureus Scientia, abbreviation, BS, or BSc), or Bachelor of Science, is an undergraduate degree awarded to graduates who have completed their undergraduate studies (known as the university department in Taiwan) in a major related to the natural sciences.

    2. BA: Bachelor of Arts (English: or, etymology comes from the Latin artium baccalaureus), which is an undergraduate bachelor's degree in education, which is awarded by most universities to students studying humanities, generally including literature, linguistics, history, geography, cultural studies and communication.

    3. MS: Master of Science (Latin: Magister Scienti; English:

    master of science;Abbreviated as or , it is a degree awarded in some countries to master's level programs in the study of science and social sciences.

    4. PhD: Doctor of Philosophy (English: Doctor of Philosophy, abbreviation:

    PhD), one of the advanced academic titles, is usually the most senior academic title in the academic structure. The owner is generally awarded a doctorate degree of philosophy after graduating from a university (bachelor's) degree or graduate school (master's degree), and after a considerable number of years of study, he or she will be awarded a doctorate degree of philosophy after completing the first academic record and passing the oral defense.

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