The difference between culture medium and petri dish, the difference between complete medium and bas

Petri dishes are placed in the medium.

The container, which is generally used to hold solid agar medium, is a round shallow dish made of plastic or glass.

The difference between a petri dish and a culture medium is probably the same as the difference between a water cup and water, one is a container and the other is the contents.

Culture medium: Culture medium is an artificially formulated nutrient for the growth and maintenance of plant tissues, animal tissues and microorganisms, and generally contains carbohydrates.

Inorganic salts (including trace elements.

Nitrogenous substances, as well as vitamins and water, etc. Different media can add some compounds that cannot be synthesized by themselves, that is, growth factors, according to the needs of the actual situation.

Different media are slightly different in terms of storage and custody due to the different raw materials used in the preparation and the different requirements for use. Generally, after being heated or moisture-absorbed, the medium is easy to be contaminated by bacteria or decomposed and deteriorated, so under normal circumstances, the medium must be protected from moisture and light, and stored in a cool place. Some media that require strict sterilization (e.g., tissue culture media) must be stored in the refrigerator at 3-6 °C if they require longer storage periods.

Since the liquid medium is not conducive to long-term storage and storage, the medium is modified into powder.

Petri dish: A petri dish is a commonly used type of labware that is mainly used for microbial or cell culture.

It consists of a flat disc-shaped bottom and a lid, usually made of plastic or glass. The material of petri dishes is basically divided into two types: plastic and glass, and glass petri dishes can be used for plant materials, microbial culture, and animal cells.

of adherent cultures. Petri dishes made of plastic may be made of polyethylene and are available for both disposable and multi-use, suitable for laboratory marking, inoculation and bacterial isolation, and for the cultivation of plant material.

Petri dishes were originally designed in 1887 by bacteriologist Julius Richard Petri, hence the name"Petri dishes"。Petri dishes are fragile and fragile, so they should be handled with caution and care when cleaning and handling. After use, the petri dish should be cleaned in time, and then stored in a safe and fixed location to prevent damage and fall.

Culture medium refers to the nutrient matrix prepared by the combination of different nutrients for microorganisms, plants or animals (or tissues) to grow and reproduce.

A petri dish is a type of labware used for microbial or cell culture that consists of a flat disc-shaped bottom and a lid, generally made of glass, but also plastic.

When cultivating microorganisms, etc., the medium is poured into a petri dish in a sterile state, and it is used to culture microorganisms after solidification.

Let's put it this way, the culture medium is like the rice you eat, and the petri dish is the bowl that holds the rice.

The culture medium is the substrate for cultivating the microorganism and provides it with nutrients, while the petri dish is the utensil for the culture medium.

Petri dishes are used to hold culture media.

Hi! Hello! Seeing your question, I feel that you are still interested in basic medium and complete medium! This is actually a question that I have been exposed to a lot during my graduate studies, so I can also share some of my thoughts and experiences.

The main difference between basic and complete media is that they differ in composition. The basic medium contains only some essential elements, including carbon source, nitrogen source, phosphorus source, vitamins, etc., while the complete medium is more comprehensive, containing all the essential elements and some auxiliary elements, such as trace elements, organic factors, etc. Therefore, the minimal medium is usually used for the cultivation of a single strain because it only provides essential elements and does not affect the growth and metabolic pathways of the microorganism being studied. Complete medium, on the other hand, is often used for the cultivation of composite cultures, as it contains elements that may be required for more cultures, ensuring that microorganisms can grow and multiply on the medium.

Of course, the selection of basic medium and complete medium should also be combined with the actual situation, determine the specific types of microorganisms you want to study, the nutritional characteristics of microorganisms, the required culture conditions and other factors, and formulate the most suitable culture plan for the right ear annihilation, so as to make the research more targeted and effective.

I myself did some experiments in microbiology and biotechnology during my college years! I remember that at that time, we had to use a specific medium to screen for mutant bacteria that lacked specific enzymes, but we found that some media did not work very well, and the colonies grew very small or could not grow at all. Later, we made some modifications, added more elements, and gradually optimized a culture medium that was most suitable for our experiments, and the effect was really much better!

At that time, it was really a small technological breakthrough for myself. I hope you can make good scientific research results as happily as I do!

A petri dish is a type of labware used for microbial or cell culture that consists of a flat disc-shaped bottom and a lid, generally made of glass or plastic.

Culture medium refers to the nutrient matrix prepared by the combination of different nutrients for microorganisms, plants or animals (or tissues) to grow and reproduce. Generally, it contains carbohydrates, nitrogenous substances, inorganic salts (including trace elements), vitamins and water.

The main components of the medium are nitrogen source, carbon source, inorganic salts, water and some growth factors, they mainly provide nutrients and growth environment for bacteria to grow and reproduce.

Water: Water is a good solvent, and the nutrients needed by bacteria must be dissolved in water first, and water is required for nutrient absorption and metabolism. Distilled water or ion-exchange water that is free of impurities is commonly used for the preparation of media.

Nitrogen source: Nitrogen source is mainly used to make up cellular substances (amino acids, proteins, nucleic acids, etc.) and nitrogen-containing metabolites. Commonly used nitrogen sources can be divided into two main categories: organic nitrogen sources and inorganic nitrogen sources.

Carbon source: The carbon source is one of the main components that make up the medium. Commonly used carbon sources are sugars, oils and fats, organic acids and low-carbon alcohols. In special cases (e.g., when carbon sources are scarce), protein hydrolysates or amino acids can also be used as carbon sources by certain strains.

Inorganic salts: Inorganic salts are indispensable nutrients for cell life activities, mainly Na+, K+, Ca2+, Mg2+, Cl, PO43-, SO42-, HCO3, etc., the main role is to maintain the osmotic balance of cell culture medium and participate in the metabolic activities of cells. In addition, by providing sodium, K+, and Ca2+, it helps cells regulate cell membrane function.

Notes:

The medium is sterilized by autoclave or filtration before use. To prevent pollution, attention should be paid to the following:

Confirm whether the working cell bank is contaminated, confirm whether the working seed batch of the virus is contaminated, and confirm whether the medium used and its added components (such as calf serum, NaHCO3, etc.) are contaminated, all of which can be confirmed and excluded by bacterial, fungal and mycoplasma sterility tests.

At the same time, the sensitivity of the sterility test medium should be verified. In actual production, a small amount of nutrient agar can be added from the prepared culture medium and cultured in a constant temperature incubator, and whether there is pollution can be observed for 48 hours.

The above content refers to: Encyclopedia - Culture Medium.

It is artificially configured for the growth of plants, animal tissues, and microorganisms for growth and maintenance. In terms of classification, it depends on what is divided, some are classified for the ingredients, and some are classified for their appearance and physical state. Like, solid media, liquid media, semi-combined media, dehydrated media, etc....

The components of the medium are nutrients, water, coagulants, inhibitors, indicators. Nutrients, nutrients provide the energy required for the growth and reproduction of bacteria, the raw materials for the synthesis of bacteria, and activate the activity of bacterial enzymes and regulate osmotic pressure, etc., the nutrients required by bacteria mainly include nitrogen sources, carbon sources, inorganic salts and growth factors, such as peptone, meat infusion, blood, and growth factors.

Characteristics of the main components of the mediumWater is a good solvent, the nutrients required by bacteria must be dissolved in water first, the absorption and metabolism of nutrients need water to be carried out, the preparation of medium is commonly used without impurities of distilled water or ion exchange water, coagulant substances, that is, excipients, when preparing solid medium, coagulant substances must be added, such as agar, gelatin, ovalbumin and serum.

The ideal coagulation substance should have the following characteristics, it is not used by bacteria, it remains solid within the range of microbial growth temperature, the temperature of the coagulation point is harmless to microorganisms, it is not destroyed by disinfection and sterilization, it has good transparency, strong adhesion, and it is currently considered that the most suitable coagulation substance is agar.

Petri dishes are autoclaved and sterilized like liquid medium, which can be poured into the petri dish with moist heat and sterilized, and it is easy to sprinkle.