What is Lamp used for and how to install it?

LAMP is an open-source web development platform based on Linux, Apache, MySQL and PHP, a programming language that is sometimes replaced by Perl or Python. The term comes from Europe, where these programs are commonly used as a standard development environment. The first name is the first letter of each program.

Each program is open source** in terms of ownership: Linux is an open system; Apache is the most versatile web server; MySQL is a relational database with additional tools for web-based management; PHP is a popular object scripting language that incorporates the best features of most other languages to make it more effective for web development. Developers use these under the Windows operating system.

MD5 checksum:

md5sum -b

Check whether the value is consistent with the content.

Start compiling and installing:

This uses DSO to compile Apache, which makes it easy to upgrade PHP and other modules without having to recompile and install it to usr local Apache every time

cd-enable-so --enable-rewrite --enable-spellingdir=/usr/lib

makemake install

At present, PCR methods are often used in domestic genetic diagnosis, involving PCR instruments, which are different from domestic and imported, and the cheap 230,000 can also be bought, and the expensive ones are more than 200,000. Laboratories and state institutions with better conditions have also tried the fluorescence quantitative PCR method, which is highly technical and must use a fluorescence quantitative PCR machine, ranging from 20 to 700,000.

Products using loop-mediated isothermal amplification technology do not require special instruments in clinical diagnosis, but the finished kit for clinical use needs to be used in a quantitative way to help researchers analyze the selection data in a quantitative way due to the selection of primers, the adjustment of primer concentration, and the optimization of reaction temperature and time in the research and development process, so we recommend the use of Rongyan's patented products LA-320C or LA-500 real-time turbidity meter.

Loop-mediated isothermal amplification, known as loop-mediatedisothermal amplification, is a new nucleic acid amplification method, which is characterized by the design of 4 specific primers for 6 regions of the target gene, under the action of strand displacement DNA polymerase (BST DNA polymerase), 60--65 constant temperature amplification, 10 9 10 10 times nucleic acid amplification can be achieved in about 15-60 minutes. It has the characteristics of simple operation, strong specificity, and easy detection of products. During DNA synthesis, the pyrophosphate ions precipitated from the DNA triphosphate substrate (DNTPS) react with the magnesium ions in the reaction solution to produce a large amount of magnesium pyrophosphate precipitate, which is white. Therefore, the turbidity can be used as an indicator of the reaction, and the amplification can be identified only by observing the white turbidity precipitate with the naked eye, without the need for tedious electrophoresis and ultraviolet observation.

Since loop-mediated isothermal amplification reactions do not require PCR instruments and expensive reagents, they have a wide range of applications.