What is the detection method for African swine fever?

1. Red blood cell adsorption test: white blood cells of healthy pigs.

Plus African swine fever.

Extracts of blood or tissue from pigs,37 cultures, as seen many red blood cells adsorbed to white blood cells, forming rosettes or mulberries.

body, it is positive.

2. Direct immunofluorescence test: fluorescence microscopy.

Observe as seen in cytoplasm.

If there is a bright fluorophore inside, it is positive.

3. Animal inoculation test.

4. Indirect immunofluorescence test: African swine fever virus is inoculated in overgrown vero cells.

on a coverslip and prepare a control of uninoculated VERO cells. After the test, the control was normal, and the bright fluorophore nuclear fluorescence dots in the cytoplasm of the sample to be tested could be judged to be positive.

5. Enzyme-linked immunosorbent assay.

When the control is established (the absorption value of the positive serum control is greater than that, the absorption value of the negative serum is less than that, and the absorption value of the sample to be tested is greater than that, it is judged to be positive.

6. Immunoelectrophoresis test: the antigen can be judged to be positive if there is a white precipitation line between the serum to be tested.

7. Indirect enzyme-linked immunoassay (ELISA) test: observed with the naked eye or under the microscope, if the spot is brown, it is positive, and if it is colorless, it is negative.

Rapid detection method for African swine fever.

The African swine fever detection method can be used as a PCR assay, which can provide rapid and accurate qualitative detection of target nucleic acids in analytes or other analytes taken from the sample to be tested.

African swine fever is referred to as ASFV, many people, especially pig professionals, mention African swine fever on the face of a big change, African swine fever is fast, acute, rapid infection, African swine fever will lead to the loss of money and property of farmers, African swine fever is an animal disease, we can use African swine fever detector to detect, in advance to do a good job of preventive measures. The African swine fever detector is used to run virus detection experiments and analyze experimental data; The instrument can be operated in the laboratory or used in field scientific experiments to perform rapid and accurate qualitative detection of target nucleic acids in analytes or other analytes taken from the sample to be tested with corresponding reagents.

African Swine Fever DetectionImmunoelectrophoresis test antigen with white precipitated lines between the serum to be tested is positive direct immunofluorescence test: cells are observed under a fluorescence microscope and are positive if there are bright fluorophores in the cytoplasm. Indirect enzyme-linked immunoplaque testing:

If the color is brown, it is indicated as a positive enzyme-linked immunosorbent assay: after the control is established, if the absorption value of the sample to be tested is greater than that, it can be judged to be positive.

Summary. Quality control standards.

1) The CT value of the negative control should be 38 or no CT value.

2) The CT value of the positive control should be 35 with a typical amplification curve.

How many people tested positive for African swine fever.

Quality control criteria: 1) The CT value of the negative control should be 38 or no CT value. 2) The CT value of the positive pair should be 35 and a typical amplification curve should appear.

Tested positive yesterday and didn't take any measures, what's the deal with being negative today?

Suspicious: The 35 ct value of the test sample and Pie is difficult to talk about 38, and there is a typical amplification curve with sheds, it should be retested, and the retest is still judged to be positive if the above results are still present, otherwise it is judged to be negative.

How do you eliminate the virus from people?

When the temperature reaches 55 for 30 minutes or 60 for 10 minutes, the ASF virus can be killed.

Is bathing also eliminating?

Can clothes be eliminated by soaking them in water at high temperatures?

Right. Okay thank you.

With an African swine fever test report, the testing proportion shall not be less than 30% What is the sampling ratio of African swine fever? - The sampling ratio of African swine fever is generally 1:20, well, this ratio should still be okay.

Is it serious. The DNA ends of African swine fever virus (ASFV) are cross-linked, and the virion has a DNA-dependent RNA polymerase. After the growth and adaptation of monocytes in pigs, they can multiply in a variety of cells, and the Vero cell line is commonly used to culture the virus. Virions are erythrocyte adsorbent.

There are at least 8 serotypes in Africa and one in Europe. Due to virus mutations, many subclinical chronic cases have emerged. The symptoms of African swine fever resemble those of classical swine fever, but there is no serologic relationship between the two.

Infected pigs carry the virus for a long time. There is no good vaccine to prevent the disease. It is found in Africa, southern Europe, Western Europe, Cuba, and Central America.

Seriously. You're sick.

Or the sampling ratio.

As long as you get it, it's very serious.

It was to draw a pig's blood and test it.

Tell me, I don't understand either.

How many pigs were smoked?

Does he know your total number of pigs?

Just one. It has to do with a few pigs.

That's fine. Don't worry ha.

Let me sell it.,Other pigs are fine.,As soon as I said the value, I was frightened.,I don't understand.,Just ask the teacher.。

- The sampling ratio of African swine fever is generally 1:20, well, this ratio should still be okay.

Yours say it's okay.

<> the pre-symptoms of African swine fever are not obvious, they can be detected by a PCR analyzer. First of all, take about 5 milliliters of live pig blood or meat products, pig slaughtering line sewage, slaughtering utensils scraping, etc., put them into sterilized centrifuge tubes, mix well, take the supernatant, and then put them into 20 ml of PCR reaction solution.