Plant tissue culture, as long as callus culture, extracts cell metabolites, and the principle is

I think so. The totipotent type of plant cells is reflected in the potential of plant cells, tissues or organs to develop into complete individuals in vitro, and is mainly reflected in plant tissue culture and plant somatic cell hybridization in plant cell engineering.

The callus in plant tissue culture can produce many drugs, spices, pigments and other substances, which are the specific functions of the cells of different plants, but the premise is: to be able to culture callus, that is, the dedifferentiation of plant cells, tissues or organs in vitro, and the dedifferentiation of calli to form callus produces metabolites in the process of redifferentiation.

In my opinion, the appearance of calli should reflect the totipotent type of plant cells.

This is a foolish opinion, and I hope you can refer to it.

I think that the accumulation of metabolites through callus can not reflect plant totipotency, the accumulation of metabolites is mainly through callus culture, so that callus can fully accumulate the energy, substances, etc., by not letting callus differentiate and make metabolites accumulate a method, this method is now widely used in the extraction of many plant metabolites, especially after callus proliferation reaches a stable period, the accumulation of metabolites is quite rapid.

High school biology books say that differentiated cells still have the potential to develop into complete individuals, and "development into complete individuals" is the embodiment of totipotency. The callus appears to be a little different from the intact individual.

The cell product is the plant tissue culture to the callus stage, and then it is cultured in large quantities, and then the cells are broken to extract the product. For example, the extraction of shikonin.

a. The callus is formed by dedifferentiation of isolated cells, and a is correct;

b. The medium for the formation of calli needs to contain organic molecules, and B is wrong;

c. The callus can form an embryo-like structure with the ability to take root and germinate after redifferentiation.

d. DNA in mitochondria can be replicated, transcribed and translated, and translation can be carried out in ribosomes, so the mitochondria and ribose transport schizomes of paracallus cells may undergo base complementary pairing, D is correct

Therefore, b

Plant tissue culture is based on the principle of totipotency of plant somatic cells, i.e. cells that have been differentiated still have the potential to develop into a complete plant

Therefore, a

Calli are formed through a process of dedifferentiation, which is achieved through the selective expression of genes.