Why plant tissue culture medium uses sucrose instead of glucose

There are two main reasons why sucrose is used as a carbon source:

1) Also used as a carbon source substance to provide energy for plant cells**, sucrose can better regulate the osmotic pressure in the medium than glucose.

The osmotic pressure formed by sucrose is significantly lower than that of glucose, so if glucose is used as a carbon source, it is easy to dehydrate plant cells and grow poorly. At the same time, the rate at which plant cells absorb sucrose is significantly slower than the rate at which glucose is absorbed, so sucrose is formed.

The osmotic pressure can be relatively stable for a long time.

2) In the process of plant tissue culture, attention should always be paid to prevent the medium from being contaminated by microorganisms. The most common carbon source for microbial growth is glucose, and sucrose is rarely utilized. Therefore, the use of sucrose as the carbon source of the medium can reduce the contamination of microorganisms to a certain extent.

The energy substance used by plant tissue cells is sucrose, which is the form of sugar transport between plant tissues, and maltose is also the same, but except for the maltose transportation when the seed germinates, most of the other plant tissue cells are transported by sucrose, and then decomposed and utilized after entering the plant cells.

For animal cells, sucrose is a macromolecule, animal cells can not only not absorb but also use, so only glucose can be used, and everyone knows that animal cells live in the liquid environment, blood sugar is an energy substance, and the sugar in blood sugar is glucose.

This question has been asked before, and university professors have also used glucose and sucrose to do plant tissue culture, both can be used, but most of the plant tissue culture is applied to industry, so there are more sucrose used to reduce cost benefits.

Sucrose is added to the medium.

Instead of glucose.

It is based on a large number of experiments. A large number of experiments have shown that sucrose can support the vigorous growth of most plant in vitro cultures, so it is widely used as a standard carbon source for plant tissue culture, and most synthetic media also use sucrose as the only carbon source. There may be several reasons for this:

Also as a carbon source and energy source, sucrose is better able to maintain a hypotonic environment in the medium than glucose. Formulate the same mass fraction.

The medium of sucrose is formed by osmotic pressure.

It is significantly lower than glucose, and if glucose is used as a carbon source, it is easy to dehydrate plant cells and grow poorly. At the same time, the rate of absorption of sucrose by plant cells is significantly slower than the rate of glucose absorption, so the osmotic pressure formed by sucrose can be relatively stable for a long time. During the process of plant tissue culture, special attention should be paid to prevent the medium from being contaminated by microorganisms.

The most suitable carbon source for microbial growth is glucose, and sucrose is less used, so the use of sucrose as the carbon source of the medium can reduce the contamination of microorganisms to a certain extent. In terms of energy**, sucrose provides more energy than glucose at the same concentration of substances.

The function has two aspects: one is to provide nutrients for plant tissue cells, and the other is to maintain permeability.

In particular, cells with the cell wall removed need to use sucrose to maintain osmotic pressure.

This mountain is used as a detective for the socks of the chain plants, which contain enzymes that hydrolyze sucrose, and the plants can use carbon sources. Many microorganisms do not contain hydrolase enzymes and cannot convert sucrose into glucose for utilization, which can inhibit the growth of microorganisms.

As a carbon source, microorganisms cannot use sucrose, but can only use glucose, which can inhibit the reproduction of microorganisms.

Sucrose is an organic substance that can be used as a source of carbon and provide nutrients.

and maintain osmotic pressure.

Provides the energy needed for plant growth.